CAJ | 학술논문

采用厌氧培养方法,在云南省昭通褐煤样品中成功地培养富集了活性厌氧细菌,并进一步开展了为期60天的生物气生成模拟实验,分析了生物气的生成规律、物质组成和成因机制.结果揭示:3件褐煤样品中均有活性厌氧细菌存在,以纤维素分解菌为主,活性硫酸盐还原菌极其微少,说明昭通盆地褐煤层具有较强还原性的生化环境,有利于产甲烷菌的繁盛;生物气的生成经历了生气最缓慢增长、显著增高、趋于减缓三个阶段,表明产甲烷菌在经历了第一阶段缓慢繁殖后,其数量和活性在第二阶段达到较高水平;3件煤样生气量和生气历程存在一定差别的原因,可能在于原煤中活性产甲烷菌数量以及显微组分组成方面存在差异,褐煤生物气生成过程至少存在两个以上的生气高峰阶段.模拟实验中产生的生物气几乎伞部由CH_4和CO_2组成,且CH_4占主要部分,几乎没有检测到重烃气.甲烷气体δ~(13)C_1和δD的平均值均处于生物甲烷碳、氢同位索组成的正常分布范围.
채용염양배양방법,재운남성소통갈매양품중성공지배양부집료활성염양세균,병진일보개전료위기60천적생물기생성모의실험,분석료생물기적생성규률、물질조성화성인궤제.결과게시:3건갈매양품중균유활성염양세균존재,이섬유소분해균위주,활성류산염환원균겁기미소,설명소통분지갈매층구유교강환원성적생화배경,유리우산갑완균적번성;생물기적생성경력료생기최완만증장、현저증고、추우감완삼개계단,표명산갑완균재경력료제일계단완만번식후,기수량화활성재제이계단체도교고수평;3건매양생기량화생기역정존재일정차별적원인,가능재우원매중활성산갑완균수량이급현미조분조성방면존재차이,갈매생물기생성과정지소존재량개이상적생기고봉계단.모의실험중산생적생물기궤호산부유CH_4화CO_2조성,차CH_4점주요부분,궤호몰유검측도중경기.갑완기체δ~(13)C_1화δD적평균치균처우생물갑완탄、경동위색조성적정상분포범위.
Using the method of anaerobic glove chamber culture,active anaerobic bacteria were successfully enriched and cultivated in brown coal samples collected from Zhaotong Basin,Yunnan Province.Simulation experiments of biogenic gas for 60 davs were further conducted to analyze the generation regularities,substance compositions and genetw mechanism of biogenic gas.The results srowed that in all three brown coal samples there existed active anaerobic bacteria,among which cellulose decomposition bacteria were in majority and sulfate-reducing bacteria were very few,which means that the biochemical circumstances in the brown coal-seams of Zhaotong Basin were reductive and favourable for the growth of methanogen.The biogenic gas generation experienced through three stages: the very slow growing stage, the evidently increasing stage and the slowing down again stage, which indicated that methanogen had high density and activity in the second stage after the slow reproduction first stage. It is considered that the differences in gas generation amount and processes among three hrown coal samples were probahly resulted from the initial quantities of the active methanogen and maceral composition in the coal. It is proposed that there were at least two peaks of biogenic gas generation from the hrown coal. Stimulated biogenic gas was mostly composed of CH_4 and CO_2, in which CH_4 was predominant and heavy hydrocarhons were hardly detected. All the mean values of δ~(13)C_1 and δD in the methane produced from three coal samples were found to be in the nonnal distrihution ranges compared to those of biogenic methane.