CAJ | 학술논문

背景:表面活性剂脱细胞的效果和骨移植材料的生物安全性与表面活性剂的选择有较大关系.因此,实验采用新型的复合表面活性剂制备脱细胞骨移植材料.目的:应用新型表面活性剂处理生物源性骨组织,并进行脱细胞效果及生物安全性评价,以期得到一种更安全、可靠的骨植入材料.方法:2种阴离子表面活性剂十二烷基苯磺酸钠和脂肪醇聚氧乙烯醚硫酸钠以及蒸馏水以质量比13:7:80的比例配制复合表面活性剂.以新鲜的牛松质骨为原料,复合表面活性剂脱脂脱细胞两步法工艺,制备新型生物源性骨植入材料.结果与结论:复合表面活性剂生物源性骨颜色呈乳白色,未见杂质,组织学及超微结构观察可见骨陷窝内细胞结构消失,骨小管空虚,胶原纤维排列整齐.生物安全性实验表明:按GB/T16886.11-1997标准急性全身毒性试验合格,溶血试验< 5%,细胞毒性试验0级.复合表面活性剂生物源性骨组织中表面活性剂的残留量低于0.1 g/L.骨长期植入实验表明:植入材料与宿主骨融合良好,24周后被机体完全吸收.说明复合表面活性剂处理的生物源性骨移植材料具有良好的生物相容性和生物降解性,是一种安全、可靠的骨植入材料.
배경:표면활성제탈세포적효과화골이식재료적생물안전성여표면활성제적선택유교대관계.인차,실험채용신형적복합표면활성제제비탈세포골이식재료.목적:응용신형표면활성제처리생물원성골조직,병진행탈세포효과급생물안전성평개,이기득도일충경안전、가고적골식입재료.방법:2충음리자표면활성제십이완기분광산납화지방순취양을희미류산납이급증류수이질량비13:7:80적비례배제복합표면활성제.이신선적우송질골위원료,복합표면활성제탈지탈세포량보법공예,제비신형생물원성골식입재료.결과여결론:복합표면활성제생물원성골안색정유백색,미견잡질,조직학급초미결구관찰가견골함와내세포결구소실,골소관공허,효원섬유배렬정제.생물안전성실험표명:안GB/T16886.11-1997표준급성전신독성시험합격,용혈시험< 5%,세포독성시험0급.복합표면활성제생물원성골조직중표면활성제적잔류량저우0.1 g/L.골장기식입실험표명:식입재료여숙주골융합량호,24주후피궤체완전흡수.설명복합표면활성제처리적생물원성골이식재료구유량호적생물상용성화생물강해성,시일충안전、가고적골식입재료.
BACKGROUND: Effect of acellular surfactant and biological safety of bone graft materials highly correlated with selection of surfactant; therefore, a novel compound surfactant was used to prepare acellular bone graft materials in this study. OBJECTIVE: To evaluate acellular effect and biological safety of bio-derived bone tissue treated by a novel surfactant in order to obtain a safe and reliable bone graft material. METHODS: Surfactant was prepared with anionic surfactant sodium dodecyl benzene sulfonate (ABS), anionic surfactant sodium fatty alcohol ether sulfate (AES) and distilled water at the ratio of 13:7:80. Fresh bovine cancellous bone and surfactant which was used to remove cells and lipid by two-step flow were used to prepare a novel bio-derived bone graft material. The histological and microscopic observations of microstructure were made. Also acute body toxicity test, hematolysis experiment, cell toxicity test and biological safety were assessed on surfactant-treated bio-dedved bone graft material (STBB). A long-term animal experiment was conducted to observe the biocompatibility and biodegradability of STBB. The ultraviolet dispersion of light luminosity method was employed to measure the residual amount of surfactant in STBB. RESULTS AND CONCLUSION: STBB was a whitish porous cancellous bone. No cell was found in bone lacuna, bone canaliculus was empty, and the collagen fiber had an order arrangement. Acute body toxicity test was qualified according to GB/116886.11-1997 standard, hematolysis experiment was < 5%, and cell toxicity test was grade 0, confirming that STBB was safe. The remaining surfactant in STBB was lower than 0.1 g/L. The long-term animal experiment demonstrated that fiber was present at 4 weeks, bone lacuna had cellular growth and the fusion of STBB and host appeared. The STBB was partial absorbed by organism at 8 weeks and completely absorbed at 24 hours. The results indicated that STBB had an excellent biocompatibility and biodegradability. As a new bone implant material, STBB was safe and dependable for transplantation.