CAJ | 학술논문

目的了解成渝地区HPV16亚型基因的一级结构特点,为成渝地区宫颈癌的防治及HPV疫苗的研制提供理论基础.方法收集宫颈癌组织标本,针对HPV16亚型分别设计L1、E6和E7基因特异性引物,用PCR方法从宫颈癌组织中获得各基因的全长序列,并构建克隆载体pGEM-T,进行序列分析,同时分别以L1、E6和E7基因序列进行进化分析.结果在102例宫颈癌标本中,99例为HPV阳性,感染率为97.1%,其中32例(32.3%)为HPV16单重感染,所占比例最高.通过与标准株比较,发现成渝两地HPV16亚型E6、E7和L1基因都存在一定程度的变异,变异率分别为90.6%、78.1%和25.0%.结论与标准序列比较,成渝地区HPV16亚型L1、E6和E7基因存在一定范围的变异,提示我们,在预防治疗成渝地区妇女宫颈癌病人以及研制开发针对西南地区的宫颈癌疫苗时,需要注意这些位点的变异,特别是一些引起氨基酸变异的位点.
목적 료해성투지구HPV16아형기인적일급결구특점,위성투지구궁경암적방치급HPV역묘적연제제공이론기출.방법 수집궁경암조직표본,침대HPV16아형분별설계L1、E6화E7기인특이성인물,용PCR방법종궁경암조직중획득각기인적전장서렬,병구건극륭재체pGEM-T,진행서렬분석,동시분별이L1、E6화E7기인서렬진행진화분석.결과 재102례궁경암표본중,99례위HPV양성,감염솔위97.1%,기중32례(32.3%)위HPV16단중감염,소점비례최고.통과여표준주비교,발현성투량지HPV16아형E6、E7화L1기인도존재일정정도적변이,변이솔분별위90.6%、78.1%화25.0%.결론 여표준서렬비교,성투지구HPV16아형L1、E6화E7기인존재일정범위적변이,제시아문,재예방치료성투지구부녀궁경암병인이급연제개발침대서남지구적궁경암역묘시,수요주의저사위점적변이,특별시일사인기안기산변이적위점.
Objective To know the primary structure character of HPV 16 isoforms in CHENG-YU district, providing the foundation for preventing and curing the cervical carcinoma. Methods E6,E7 and L1 gene were amplified from human cervical car-cinoma by PCR. The products of PCR were ligated into pGEM-T vector and used to transform E. coli DH5α. At last, the sequenceof the insert was determined by an automated DNA sequencer. Results Of the 102 cervical carcinoma specimens, 97.1%(99/102) were positive for HPV infection, including 32. 3% (32/99) HPV16 single infection . Compared with the prototype, a certain variation was found in HPV isoforms of E6, E7, L1, the variation ratio was 90. 6%, 78. 1 % , and 25. 0% respectively. Conclusion Compared with the prototype, the nucleotide sequence of the HPV16 E6,E7 and L1 gene from cervical carcinoma biopsis in CHENG-YU district shows a certain point mutations. We should pay attention to this mutations in preventing and curing cervical carcinoma in CHENG-YU district.