CAJ | 학술논문

背景:许旺细胞能够分泌多种神经营养因子,促进脊髓损伤功能的恢复.但异体许旺细胞移植可引发自身免疫反应,且在移植方式上,局部移植无法避免二次损伤,静脉移植虽可以透过血脊髓屏障到达损伤局部,但不能达到有效的治疗浓度.目的:探讨经蛛网膜下腔移植自体激活许旺细胞对脊髓损伤大鼠功能恢复的影响.方法:66只大鼠均建立脊髓损伤模型,造模后随机分为3组,自体激活许旺细胞组通过结扎单侧隐神经从而激活许旺细胞,自体未激活许旺细胞组、模型对照组仅在相同部位手术但不结扎神经.切除各组手术远端1 cm神经,采用组织块法进行许旺细胞的体外分离培养及纯化.1周后,自体激活许旺细胞组、自体未激活许旺细胞组分别通过蛛网膜下腔注入经Hoechst33342标记的对应许旺细胞悬液,模型对照组仅注入等量DMEM.对脊髓损伤后肢体功能的恢复进行BBB运动功能评分及脚印分析,通过苏木精-伊红染色和GFAP染色从组织学角度评价脊髓损伤恢复情况.结果与结论:从术后第4周开始,自体激活许旺细胞组BBB后肢功能评分明显优于另两组(P＜0.05).移植后2周,可见迁移至大鼠脊髓损伤局部的许旺细胞.与自体未激活许旺细胞组比较,移植后5周自体激活许旺细胞组的前后足中心距离、后肢第3足趾外旋角度均显著减小(P＜0.05),移植后13周损伤区胶质瘢痕面积明显减小(P＜0.05),损伤区空洞面积明显减小(P＜0.05).证实经蛛网膜下腔移植自体激活许旺细胞可以促进脊髓损伤的恢复.
배경:허왕세포능구분비다충신경영양인자,촉진척수손상공능적회복.단이체허왕세포이식가인발자신면역반응,차재이식방식상,국부이식무법피면이차손상,정맥이식수가이투과혈척수병장도체손상국부,단불능체도유효적치료농도.목적:탐토경주망막하강이식자체격활허왕세포대척수손상대서공능회복적영향.방법:66지대서균건립척수손상모형,조모후수궤분위3조,자체격활허왕세포조통과결찰단측은신경종이격활허왕세포,자체미격활허왕세포조、모형대조조부재상동부위수술단불결찰신경.절제각조수술원단1 cm신경,채용조직괴법진행허왕세포적체외분리배양급순화.1주후,자체격활허왕세포조、자체미격활허왕세포조분별통과주망막하강주입경Hoechst33342표기적대응허왕세포현액,모형대조조부주입등량DMEM.대척수손상후지체공능적회복진행BBB운동공능평분급각인분석,통과소목정-이홍염색화GFAP염색종조직학각도평개척수손상회복정황.결과여결론:종술후제4주개시,자체격활허왕세포조BBB후지공능평분명현우우령량조(P＜0.05).이식후2주,가견천이지대서척수손상국부적허왕세포.여자체미격활허왕세포조비교,이식후5주자체격활허왕세포조적전후족중심거리、후지제3족지외선각도균현저감소(P＜0.05),이식후13주손상구효질반흔면적명현감소(P＜0.05),손상구공동면적명현감소(P＜0.05).증실경주망막하강이식자체격활허왕세포가이촉진척수손상적회복.
BACKGROUND:Schwann cells can secrete various neurotrophic factors,and promote functional recovery of injured spinal cord.However,xenogenic Schwann cells transplantation may induce autoimmune response.Moreover,local transplantation results in secondary injury.Vein transplantation may reached injury site passing the blood spinal cord barrier,but the treatment concentration is not effective.OBJECTIVE:To investigate the therapeutic effects of transplantation of autologous activated Schwann Cells(AASCs)via subarachnoid space on spinal cord injury(SCI)in rats.METHODS:A total of 66 rats were used to establish SCI models,and the model rats were randomly divided into 3 groups.The unilateral saphenous nerves of rats were ligated directly for 1 week to activate Schwann cells,but inactivated and model control groups were not subjected to nerve ligation.1 cm nerve was excised from distal end of each group,and Schwann cells were isolated and cultured by tissue mass method.The AASCs,autologous Schwann cells(ASCs)were injected with corresponding Hoechst33342-labeted SCs suspension,but the model control group was injected with DMEM injection.The basso beattie bresnahan(BBB)score and footprint analysis,as well as HE and GFAP immunohistochemistry staining were performed to evaluate functional recovery of rat hind limbs.RESULTS AND CONCLUSION:On 4 weeks after injury,BBB scores of AASCs were significantly superior to the other groups (P＜0.05).Two weeks after transplantation,some SCs migrated to injured spinal cord.Compared with ASCs group,the center distance of forward and hind feet and extorsion angle of the third toe of hind limb were significantly reduced in the AASCs group at 5 weeks(P＜0.05),the glial scar area was significantly decreased at 13 weeks(P＜0.05),and the cavity area of injured region was signiflcentJy diminished(P＜0.05).Results show that AASCs transplantation via subarachnoid space promoted functional recovery after SCI in rats.