国际病毒学杂志
國際病毒學雜誌
국제병독학잡지
INTERNATIONAL JOURNAL OF VIROLOGY
2012年
1期
15-19
,共5页
秦辉%程险峰%滕旭%徐维祯%房勇%谷鸿喜%李迪
秦輝%程險峰%滕旭%徐維禎%房勇%穀鴻喜%李迪
진휘%정험봉%등욱%서유정%방용%곡홍희%리적
HBV%拉米夫定耐药株%YMDD变异%基因型%BCP变异
HBV%拉米伕定耐藥株%YMDD變異%基因型%BCP變異
HBV%랍미부정내약주%YMDD변이%기인형%BCP변이
Hepatitis B virus%Lamivudine-resistant strain%YMDD mutation%Genotype%BCP mutation
目的 探讨黑龙江省乙型肝炎病毒(HBV)拉米夫定耐药株YMDD变异与基因型、HBV BCP变异之间的相关性.方法 对收集的245例经拉米夫定治疗的慢性乙型肝炎患者血清标本,采用多重PCR法检测HBV的基因型;荧光标记杂交双探针PCR融解曲线法(FH-PCR-MC)检测HBV YMDD变异及变异类型;巢式PCR法扩增HBV C基因区,测序分析BCP位点变异并进行统计学分析.结果 在HBV YMDD野生株、变异株中,基因型B、C、B与C混合型分别为8.8%、89.2%、2.0%;6.3%、93%、0.7%,基因型构成比无统计学的差异.HBV BCP的变异率在YMDD野生株与变异株分别为69.6%、76.9%,无统计学的差异.在HBV YMDD野生株中,HBV B基因型BCP的变异率22.2%,C基因型BCP的变异率73.6%存在统计学差异(P<0.01),但在YMDD变异株中,无统计学的差异.在YIDD、YVDD变异株HBV BCP变异率分别为83.0%和64.6%,存在统计学的差异(P<0.05).结论 在HBV YMDD野生株中,与B基因型相比,C基因型更易发生BCP变异;YMDD变异株中,BCP变异率在B、C基因型间无统计学差异,BCP变异在变异类型间(YIDD、YVDD)存在统计学差异;与YVDD变异株相比较,YIDD变异株易发生HBV BCP的变异.
目的 探討黑龍江省乙型肝炎病毒(HBV)拉米伕定耐藥株YMDD變異與基因型、HBV BCP變異之間的相關性.方法 對收集的245例經拉米伕定治療的慢性乙型肝炎患者血清標本,採用多重PCR法檢測HBV的基因型;熒光標記雜交雙探針PCR融解麯線法(FH-PCR-MC)檢測HBV YMDD變異及變異類型;巢式PCR法擴增HBV C基因區,測序分析BCP位點變異併進行統計學分析.結果 在HBV YMDD野生株、變異株中,基因型B、C、B與C混閤型分彆為8.8%、89.2%、2.0%;6.3%、93%、0.7%,基因型構成比無統計學的差異.HBV BCP的變異率在YMDD野生株與變異株分彆為69.6%、76.9%,無統計學的差異.在HBV YMDD野生株中,HBV B基因型BCP的變異率22.2%,C基因型BCP的變異率73.6%存在統計學差異(P<0.01),但在YMDD變異株中,無統計學的差異.在YIDD、YVDD變異株HBV BCP變異率分彆為83.0%和64.6%,存在統計學的差異(P<0.05).結論 在HBV YMDD野生株中,與B基因型相比,C基因型更易髮生BCP變異;YMDD變異株中,BCP變異率在B、C基因型間無統計學差異,BCP變異在變異類型間(YIDD、YVDD)存在統計學差異;與YVDD變異株相比較,YIDD變異株易髮生HBV BCP的變異.
목적 탐토흑룡강성을형간염병독(HBV)랍미부정내약주YMDD변이여기인형、HBV BCP변이지간적상관성.방법 대수집적245례경랍미부정치료적만성을형간염환자혈청표본,채용다중PCR법검측HBV적기인형;형광표기잡교쌍탐침PCR융해곡선법(FH-PCR-MC)검측HBV YMDD변이급변이류형;소식PCR법확증HBV C기인구,측서분석BCP위점변이병진행통계학분석.결과 재HBV YMDD야생주、변이주중,기인형B、C、B여C혼합형분별위8.8%、89.2%、2.0%;6.3%、93%、0.7%,기인형구성비무통계학적차이.HBV BCP적변이솔재YMDD야생주여변이주분별위69.6%、76.9%,무통계학적차이.재HBV YMDD야생주중,HBV B기인형BCP적변이솔22.2%,C기인형BCP적변이솔73.6%존재통계학차이(P<0.01),단재YMDD변이주중,무통계학적차이.재YIDD、YVDD변이주HBV BCP변이솔분별위83.0%화64.6%,존재통계학적차이(P<0.05).결론 재HBV YMDD야생주중,여B기인형상비,C기인형경역발생BCP변이;YMDD변이주중,BCP변이솔재B、C기인형간무통계학차이,BCP변이재변이류형간(YIDD、YVDD)존재통계학차이;여YVDD변이주상비교,YIDD변이주역발생HBV BCP적변이.
Objective To investigate the correlation between BCP mutations,genotypes and HBV Lamivudine-resistant YMDD mutation in Heilongjiang province. Methods The HBV DNA in 245 sera samples were collected from chronic hepatitis B patients who had been treated with lamivudine.HBV genotypes and YMDD mutations were detected by multiplex PCR and FH-PCR-MC respectively.HBV C gene region was amplified by nest PCR,then BCP mutations were analyzed.Results There were no statistical differences for the ratio of genotype between YMDD wild type(8.8% with genotype B,89.2% with genotype C and 2.0% with B,C mixed genotype) and YMDD mutant type(6.3% with genotype B,93.0% with genotype C and 0.7% with B,C mixed genotype).The ratio of BCP mutations in YMDD mutant type and YMDD wild type were 76.9% and 69.6% respectively and there were no statistical differences between them.In YMDD wild strains,BCP mutations occurred more frequently in genotype C than in genotype B ( P < 0.01 ).While,in YMDD mutant type,there was no significant difference.The ratio of BCP mutations were 83.0% and 64.6% between YIDD and YVDD mutants,respectively,there were statistical differences between them (P < 0.05).Conclusion There was a significant difference of the HBV BCP mutation with YMDD wild strains between the genotypes B and C.Mutation rate of the HBV BCP with YMDD mutants showed no significant difference between genotypes B and C.There was a significant difference of the HBV BCP mutation with YMDD mutants between the YIDD mutation type and YVDD mutation type.