CAJ | 학술논문

目的探讨外周血CD8+CD28- T细胞在强直性脊柱炎(AS)发病中的作用.方法应用流式细胞术检测50例AS患者和21名正常人外周血CD3+CD8+ T细胞表面CD28分子的表达,同时用免疫散射比浊法检测相应血清中的C反应蛋白(CRP).结果 AS患者外周血中的CD8+CD28- T细胞的表达明显高于正常人[(18±6)%对(14±5)%,P=0.020],患者外周血CD3+、CD8+CD28+ T细胞的表达明显低于正常人[(65±9)%对(69±8)%,P=0.039];[(15±5)%对(18±4)%,P=0.038],CD8+ T细胞的表达与正常人相比差异无统计学意义(P＞0.05).结论 AS患者外周血中的CD8+CD28- T细胞的表达增加,可能参与了AS的疾病过程.
목적 탐토외주혈CD8+CD28- T세포재강직성척주염(AS)발병중적작용.방법 응용류식세포술검측50례AS환자화21명정상인외주혈CD3+CD8+ T세포표면CD28분자적표체,동시용면역산사비탁법검측상응혈청중적C반응단백(CRP).결과 AS환자외주혈중적CD8+CD28- T세포적표체명현고우정상인[(18±6)%대(14±5)%,P=0.020],환자외주혈CD3+、CD8+CD28+ T세포적표체명현저우정상인[(65±9)%대(69±8)%,P=0.039];[(15±5)%대(18±4)%,P=0.038],CD8+ T세포적표체여정상인상비차이무통계학의의(P＞0.05).결론 AS환자외주혈중적CD8+CD28- T세포적표체증가,가능삼여료AS적질병과정.
Objective To examine the expression of CD8+CD28- T cells in the peripheral blood and explore their roles in the pathogenesis of AS. Methods The expression of CD8+CD28- T cells obtained from 50 AS patients and 21 healthy controls were assayed by flow cytometry. CRP was also measured. Results The percentage of peripheral blood CD8+CD28- T cells in patients with AS was significantly increased compared to normal individuals [(18±6)% vs (14±5)%, P=0.020], while the percentage of peripheral blood CD3+,CD8+ CD28+ T cells in patients with AS was significantly decreased[ (65±9)% vs (69±8)%, P=0.039]; [ (15±5)% vs(18±4)%, P=0.038]. No difference was found in CD8+ T cells between patients with AS and normal individuals(P＞0.05). Conclusion The percentage of peripheral blood CD8+CD28- T cells in patients with AS is significantly increased comparing to normal individuals. This suggests that CD8+CD28- T cells may play an important role in the pathogenesis of AS.