中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2008年
4期
296-298
,共3页
张春玲%厉保秋%尹洪银%赵秀兰
張春玲%厲保鞦%尹洪銀%趙秀蘭
장춘령%려보추%윤홍은%조수란
芦荟甙%一氧化氮%便秘
蘆薈甙%一氧化氮%便祕
호회대%일양화담%편비
BARBALOIN%Nitric oxide%Constipation
目的 观察芦荟对老年便秘小鼠排便作用及肠运动功能的影响,并探讨其作用机制.方法 高效液相色谱法测定芦荟粉中芦荟苷的含量,给老年小鼠灌服复方地芬诺酯混悬液,建立便秘模型,通过观察小鼠首次排黑便时间、粪便性状、粪便含水率及小肠墨汁推进百分率,评价芦荟对老年小鼠排便作用及肠运动功能的影响,并测定小鼠血清一氧化氮(NO)含量,探讨芦荟促进肠运动的机制.结果 与模型对照组比较,芦荟低、中、高剂量组均能不同程度缩短便秘小鼠的首便时间[分别为(259.4±49.2)、(195.1±38.1)、(180.4±43.5)、(77.9±37.6)min],增加6 h黑便粒数及粪便含水率,芦荟中、高剂量组均能提高小鼠的小肠墨汁推进率.芦荟高剂量组血清NO含量与模型对照组比较[分别为(66.21±13.70)μmol/L与(84.67±22.94)μmol/L],差异有统计学意义(P＜0.05).相关性分析表明,血清NO含量与小肠墨汁推进率呈负相关(r=-0.346,P＜0.05).结论 芦荟能增加老年便秘小鼠的肠运动和粪便含水率,起到治疗便秘的作用,其作用机制可能与调节体内NO水平有关.
目的 觀察蘆薈對老年便祕小鼠排便作用及腸運動功能的影響,併探討其作用機製.方法 高效液相色譜法測定蘆薈粉中蘆薈苷的含量,給老年小鼠灌服複方地芬諾酯混懸液,建立便祕模型,通過觀察小鼠首次排黑便時間、糞便性狀、糞便含水率及小腸墨汁推進百分率,評價蘆薈對老年小鼠排便作用及腸運動功能的影響,併測定小鼠血清一氧化氮(NO)含量,探討蘆薈促進腸運動的機製.結果 與模型對照組比較,蘆薈低、中、高劑量組均能不同程度縮短便祕小鼠的首便時間[分彆為(259.4±49.2)、(195.1±38.1)、(180.4±43.5)、(77.9±37.6)min],增加6 h黑便粒數及糞便含水率,蘆薈中、高劑量組均能提高小鼠的小腸墨汁推進率.蘆薈高劑量組血清NO含量與模型對照組比較[分彆為(66.21±13.70)μmol/L與(84.67±22.94)μmol/L],差異有統計學意義(P＜0.05).相關性分析錶明,血清NO含量與小腸墨汁推進率呈負相關(r=-0.346,P＜0.05).結論 蘆薈能增加老年便祕小鼠的腸運動和糞便含水率,起到治療便祕的作用,其作用機製可能與調節體內NO水平有關.
목적 관찰호회대노년편비소서배편작용급장운동공능적영향,병탐토기작용궤제.방법 고효액상색보법측정호회분중호회감적함량,급노년소서관복복방지분낙지혼현액,건립편비모형,통과관찰소서수차배흑편시간、분편성상、분편함수솔급소장묵즙추진백분솔,평개호회대노년소서배편작용급장운동공능적영향,병측정소서혈청일양화담(NO)함량,탐토호회촉진장운동적궤제.결과 여모형대조조비교,호회저、중、고제량조균능불동정도축단편비소서적수편시간[분별위(259.4±49.2)、(195.1±38.1)、(180.4±43.5)、(77.9±37.6)min],증가6 h흑편립수급분편함수솔,호회중、고제량조균능제고소서적소장묵즙추진솔.호회고제량조혈청NO함량여모형대조조비교[분별위(66.21±13.70)μmol/L여(84.67±22.94)μmol/L],차이유통계학의의(P＜0.05).상관성분석표명,혈청NO함량여소장묵즙추진솔정부상관(r=-0.346,P＜0.05).결론 호회능증가노년편비소서적장운동화분편함수솔,기도치료편비적작용,기작용궤제가능여조절체내NO수평유관.
Objective To observe the effect of aloe on intestine motility in the old costive mice and investigate the mechanism for aloe promoting an intestinal motility. Methods The content of aloin in aloe powder was determined by high performance liquid chromatography(HPLC).The old mice aged 15 months were randomly divided into 6 groups(10 in each group):blank control,positive control,constipation model,low-dose aloe,middle-dose aloe,high-dose aloe plus model.The mice of with equivalent volume of distilled water.On the eighth day,the mice except control group were given Compound Diphenoxylate to establish constipation model. With the black Indian ink as marker,the first time of black stool discharge,the character and weight of the stool,and the ink propulsion rate by intestines in mice were observed respectively.The serum level of nitric oxide(NO)was determined by spectrophotometry. Results The content of aloin in aloe powder was 0.266%.Compared with constipation model group,aloe groups in different dose decreased the first black stool time and increased stool grains and weight in 6 hours of constipated mice.The ink propulsion rates of intestines in the aloe groups were significantly higher than that of model group as well.The NO level in high-dose aloe group decreased more significantly compared with model group(P＜0.05),and there was a negative correlation between the serum NO level and propulsion rate of intestines(r=-0.346.P＜O.05). Conclusions Aloe could promote the mobility of intestine and ameliorate the constipation of mice,which might attribute to the decrease of the serum NO level.
