CAJ | 학술논문

目的探讨加减右归饮对兔膝关节骨关节炎软骨细胞凋亡的影响.方法 24只青紫蓝兔完全随机分为正常组、模型组、中医组和阳性对照组,按兔膝关节制动引起关节软骨退变的方法造模,8周后处死动物制备标本,光镜、透射电镜观察关节软骨形态学变化,流式细胞术检测软骨细胞凋亡率.结果中药组细胞凋亡率明显低于模型组及阳性对照组,差异有统计学意义(P＜0.05).结论加减右归饮具有抑制软骨细胞凋亡的作用.
목적 탐토가감우귀음대토슬관절골관절염연골세포조망적영향.방법 24지청자람토완전수궤분위정상조、모형조、중의조화양성대조조,안토슬관절제동인기관절연골퇴변적방법조모,8주후처사동물제비표본,광경、투사전경관찰관절연골형태학변화,류식세포술검측연골세포조망솔.결과 중약조세포조망솔명현저우모형조급양성대조조,차이유통계학의의(P＜0.05).결론 가감우귀음구유억제연골세포조망적작용.
Objective To observe the changes of chondrocytes apoptosis of experimental osteoarthritis (OA)in rabbits.Methods The left knees of rabbits were immobilized in full extension for eight weeks using plaster cast.Changes of morphology of chondrocyte were observed by light microscopy and transmission electron microscopy and examined by flow cytometry for apoptosis percentage.Results Compared with others,apoptosis cell index of JiaJian YouGiu Ying group had significant differences(P＜0.05).Conclusion Jia Jian You Gui Yin decreases the apoptosis of chondrocytes and shows therapeutic effect for experimental osteoarthritis.