中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2010年
2期
129-131
,共3页
石建华%汪万英%李阳
石建華%汪萬英%李暘
석건화%왕만영%리양
基质金属蛋白酶9%金属蛋白酶组织抑制因子1%NF-κB%原位杂交%免疫组织化学
基質金屬蛋白酶9%金屬蛋白酶組織抑製因子1%NF-κB%原位雜交%免疫組織化學
기질금속단백매9%금속단백매조직억제인자1%NF-κB%원위잡교%면역조직화학
Matrix metalloproteinase-9%Tissue inhibitor of metalloproteinnse-1%NF-κB%In situ hybridization%Immunohistochemistry
采用免疫组织化学和原位杂交方法检测157例甲状腺乳头状癌和30例甲状腺乳头状瘤组织中基质金属蛋白酶9(MMP-9)、金属蛋白酶组织抑制因子1(TIMP-1)及NF-κB的表达.结果显示甲状腺乳头状癌中MMP-9及NF-κB的蛋白及mRNA表达阳性率均明显高于乳头状瘤(均P<0.01).MMP-9及NF-κB蛋白和mRNA在甲状腺乳头状癌随着病理分级增高而显著增加(P<0.01);并且颈部淋巴结有转移者均高于无转移者(P<0.01).而TIMP-1蛋白及mRNA阳性表达率与MMP-9阳性表达率相反.
採用免疫組織化學和原位雜交方法檢測157例甲狀腺乳頭狀癌和30例甲狀腺乳頭狀瘤組織中基質金屬蛋白酶9(MMP-9)、金屬蛋白酶組織抑製因子1(TIMP-1)及NF-κB的錶達.結果顯示甲狀腺乳頭狀癌中MMP-9及NF-κB的蛋白及mRNA錶達暘性率均明顯高于乳頭狀瘤(均P<0.01).MMP-9及NF-κB蛋白和mRNA在甲狀腺乳頭狀癌隨著病理分級增高而顯著增加(P<0.01);併且頸部淋巴結有轉移者均高于無轉移者(P<0.01).而TIMP-1蛋白及mRNA暘性錶達率與MMP-9暘性錶達率相反.
채용면역조직화학화원위잡교방법검측157례갑상선유두상암화30례갑상선유두상류조직중기질금속단백매9(MMP-9)、금속단백매조직억제인자1(TIMP-1)급NF-κB적표체.결과현시갑상선유두상암중MMP-9급NF-κB적단백급mRNA표체양성솔균명현고우유두상류(균P<0.01).MMP-9급NF-κB단백화mRNA재갑상선유두상암수착병리분급증고이현저증가(P<0.01);병차경부림파결유전이자균고우무전이자(P<0.01).이TIMP-1단백급mRNA양성표체솔여MMP-9양성표체솔상반.
The expressions of matrix metalloproteinnse-9 (MMP-9), tissue inhibitor of metalloproteinase-1 (TIMP-1) ,and NF-κB in 157 cases of papillary thyroid cancer and 30 cases of papillary thyroid tumors were detected by immunohistochemistry and in situ hybridization. The expressions of MMP-9 and NF-κB protein and mRNA in papillary thyroid cancers were significantly higher than those in benign tumors (all P <0.01), and increased according to the pathological grading (P<0.01). The expressions of MMP-9 and NF-κB protein and mRNA in papillary thyroid cancers with cervical lymph node metastasis were higher than those without metastasis (both P <0.01). The expressions of TIMP-1 protein and mRNA appeared an opposite direction of MMP-9 expression.
