国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2012年
3期
224-226
,共3页
生化片%异鼠李素-3-O-新橙皮糖苷%反相高效液相色谱法
生化片%異鼠李素-3-O-新橙皮糖苷%反相高效液相色譜法
생화편%이서리소-3-O-신등피당감%반상고효액상색보법
ShenghuaTablets%Isorhamnetin-3-O-nehesperidine%RP-HPLC
目的 建立测定生化片中异鼠李素-3-O-新橙皮糖苷含量的RP-HPLC方法.方法 采用Luna-C18柱(4.6 mm×250 mm,5μm),以乙腈:0.4％磷酸(10∶90)为流动相,流速0.8 ml/min,柱温30℃,检测波长254 nm.结果异鼠李素-3-O-新橙皮糖苷在0.056～1.12 mg/ml浓度范围内有良好线性关系(r=0.9997),平均加样回收率为98.0％,RSD小于2.0％.结论 本方法简便快速,重复性好,可用于该制剂的质量控制.
目的 建立測定生化片中異鼠李素-3-O-新橙皮糖苷含量的RP-HPLC方法.方法 採用Luna-C18柱(4.6 mm×250 mm,5μm),以乙腈:0.4％燐痠(10∶90)為流動相,流速0.8 ml/min,柱溫30℃,檢測波長254 nm.結果異鼠李素-3-O-新橙皮糖苷在0.056～1.12 mg/ml濃度範圍內有良好線性關繫(r=0.9997),平均加樣迴收率為98.0％,RSD小于2.0％.結論 本方法簡便快速,重複性好,可用于該製劑的質量控製.
목적 건립측정생화편중이서리소-3-O-신등피당감함량적RP-HPLC방법.방법 채용Luna-C18주(4.6 mm×250 mm,5μm),이을정:0.4％린산(10∶90)위류동상,류속0.8 ml/min,주온30℃,검측파장254 nm.결과이서리소-3-O-신등피당감재0.056～1.12 mg/ml농도범위내유량호선성관계(r=0.9997),평균가양회수솔위98.0％,RSD소우2.0％.결론 본방법간편쾌속,중복성호,가용우해제제적질량공제.
Objective To establish a HPLC method for the determination of Isorhamnetin3-O-nehesperidinein Shenghua Tablets.Methods The RP-HPLC system was consisted of the Luna-C18(4.6 mm×250 mm,5 μm) column,the mobile phase was acetonitrile -0.4％H3PO4 (10 ∶ 90),the flow rate was set at 0.8 ml/min,the column temperature was 30*℃,and the UV detector was 254 nm.Results The linear response range was 0.056～ 1.12 mg/ml (r=0.9997).The average recovery of mangiferin was 98.0％,and RSD＜2％.Conclusion The method was simple,rapid,accurate and repeatable.It could be applied in the determination of Isorhamnetin-3-O-nehesperidine in Shenghua Tablets.
