国际临床化学联合会酶学测定参考方法实验室网络的初步建立
국제림상화학연합회매학측정삼고방법실험실망락적초보건립
Performance of IFCC enzyme reference method laboratory network in China
  • 万方数据
    中华检验医学杂志 중화검험의학잡지
    CHINESE JOURNAL OF LABORATORY MEDICINE
    2008年 3期 258-263 ,共6页

    汪静%张传宝%居漪%陈宝荣%张曼%徐国宾%但万春%周跃国%宋耀虹%申子瑜%郭健%陈文祥%杨振华

    왕정%장전보%거의%진보영%장만%서국빈%단만춘%주약국%송요홍%신자유%곽건%진문상%양진화

    酶试验%参考标准%实验室,医院%计算机通信网络 酶試驗%參攷標準%實驗室,醫院%計算機通信網絡
    매시험%삼고표준%실험실,의원%계산궤통신망락
    Enzyme tests%Reference standards%Laboratories,hospital%Computer communication networks
    目的 总结我国实验室运行国际临床化学联合会(IFCC)酶学测定参考方法情况和参加IFCC参考实验室室间质评(RELA)结果,了解我国酶学参考测量水平.方法 目前国内有8家实验室运行全部或部分IFCC酶学参考方法.各实验室按IFCC参考方法建立测量程序,评价各自程序的精密度与准确性,精密度评价按美国临床和实验室标准协会(CLSI)EP-5进行,准确度评价采用国际有证参考物质.7家实验室参加2006年RELA.结果 各实验室目前能达到的批内变异系数(CV)均在1.5%以内,批间精密度在2%以内.部分实验室进行了有证参考物质分析,测定结果偏倚在给定的不确定度范围内.我国酶学实验室在2006年RELA中具有与除外组相似的室间CV,只是样本A乳酸脱氢酶(LDH)及样本B ALT的室间CV较大,但剔除可能离群值后室问CV与除外组相似.结论 中国酶学实验室经过5年的发展,现在无论从硬件配置还是技术力量上均已接近国外同类实验室,我国临床酶学参考实验室网络已初步建立.
    목적 총결아국실험실운행국제림상화학연합회(IFCC)매학측정삼고방법정황화삼가IFCC삼고실험실실간질평(RELA)결과,료해아국매학삼고측량수평.방법 목전국내유8가실험실운행전부혹부분IFCC매학삼고방법.각실험실안IFCC삼고방법건립측량정서,평개각자정서적정밀도여준학성,정밀도평개안미국림상화실험실표준협회(CLSI)EP-5진행,준학도평개채용국제유증삼고물질.7가실험실삼가2006년RELA.결과 각실험실목전능체도적비내변이계수(CV)균재1.5%이내,비간정밀도재2%이내.부분실험실진행료유증삼고물질분석,측정결과편의재급정적불학정도범위내.아국매학실험실재2006년RELA중구유여제외조상사적실간CV,지시양본A유산탈경매(LDH)급양본B ALT적실간CV교대,단척제가능리군치후실문CV여제외조상사.결론 중국매학실험실경과5년적발전,현재무론종경건배치환시기술역량상균이접근국외동류실험실,아국림상매학삼고실험실망락이초보건립.
    Objective To review the performance of the IFCC enzyme reference methods in China laboratories and to evaluate the competence of enzyme reference measurement in China.Methods The Intemational Federation of Clinical Chemistry and Laboratory Medicine(IFCC)enzyme reference methods were performed in 8 China laboratories.Reference procedures were developed by each laboratory and the performances were evaluated.The Clinical and Laboratory Stadards Institute(CLSI)EP-5 protocol was used for the evaluation of precision and certified reference materials(CRMS)were used for the verification of trueness.Seven of the 8 laboratories participated in the 2006 IFCC external quality assessment program for reference laboratories(RELA)for the assessment of the measurement performance.Results Within-run CVs of less than 1.5%and between-run CVs of less than 2%were achieved bv all the China laboratories.Results on CRMs by some of the laboratories agreed with the certified value within the stated uncertainty.In the 2006 RELA,the averages and the interlaboratory CVs of the China laboratories were similar to those of international laboratories.Conclusion A preliminary enzyme reference method laboratory network has been established in China.
    원문보기 원문다운로드 사이트로이동
저자의 최근 논문