宁波大学学报（理工版）
寧波大學學報（理工版）
저파대학학보（리공판）
JOURNAL OF NINGBO UNIVERSITY(NSEE)
2000年
2期
96-101
,共6页
神经营养活性%人胶质瘤细胞SWO-38条件培养基%小脑皮质神经元%自动比色微量分析%Sephacryl S-200-HR凝胶层析
神經營養活性%人膠質瘤細胞SWO-38條件培養基%小腦皮質神經元%自動比色微量分析%Sephacryl S-200-HR凝膠層析
신경영양활성%인효질류세포SWO-38조건배양기%소뇌피질신경원%자동비색미량분석%Sephacryl S-200-HR응효층석
neurotrophic activity%human glioma cell line conditioned medium%cerebellar corticalneurons%automated colorimetric microassay%Sephacryl S-200-HR gel chromatography
制备了人胶质瘤细胞SWO-38条件培养基(SWO-38 CM)，经超滤浓缩，截留分子量大于10 kD的SWO-38 CM，并运用快速自动比色微量分析法检测其对体外培养的SD大鼠小脑皮质神经元的作用．结果表明，分子量大于10 kD的SWO-38 CM对神经元的作用，与对照组比较有明显的神经营养活性．分子量大于10 kD的SWO-38 CM经Sephacry1S-200-HR凝胶层析并结合生物活性鉴定，获得了具有神经营养活性的第Ⅱ峰洗脱液．
製備瞭人膠質瘤細胞SWO-38條件培養基(SWO-38 CM)，經超濾濃縮，截留分子量大于10 kD的SWO-38 CM，併運用快速自動比色微量分析法檢測其對體外培養的SD大鼠小腦皮質神經元的作用．結果錶明，分子量大于10 kD的SWO-38 CM對神經元的作用，與對照組比較有明顯的神經營養活性．分子量大于10 kD的SWO-38 CM經Sephacry1S-200-HR凝膠層析併結閤生物活性鑒定，穫得瞭具有神經營養活性的第Ⅱ峰洗脫液．
제비료인효질류세포SWO-38조건배양기(SWO-38 CM)，경초려농축，절류분자량대우10 kD적SWO-38 CM，병운용쾌속자동비색미량분석법검측기대체외배양적SD대서소뇌피질신경원적작용．결과표명，분자량대우10 kD적SWO-38 CM대신경원적작용，여대조조비교유명현적신경영양활성．분자량대우10 kD적SWO-38 CM경Sephacry1S-200-HR응효층석병결합생물활성감정，획득료구유신경영양활성적제Ⅱ봉세탈액．
Human glioma cell line (SWO-38) conditioned medium (SWO-38 CM)was prepared,ultratiltrated and concentrated, so the fraction of molecular weight (MW)>10 kD from SWO-38CM was obtained. The effect of SWO-38 CM fractions (MW>10 kD and MW<10kD)on theactivity of cultured cerebellar cortical neurons of Spraque-Dawley rat was measured by a rapidautomated colorimetric microassay, respectively. The data indicated that SWO-38 CM fraction(MW>10 kD) possessed significant neurotrophic activity to the neurons, as compared to thecontrol (P<0.05). Additionally, the active fraction of SWO-38 CM was purified further bySephacryl S-200-HR gel chromatography and peak Ⅱ was only identified to possess neurotrophicactivity.
