中国糖尿病杂志
中國糖尿病雜誌
중국당뇨병잡지
CHINESE JOURNAL OF DIABETES
2008年
12期
746-748,758
,共4页
迟铖%金苗苗%母义明%汪保安%马晓莉%贾贺堂%杨丽娟%窦京涛%陆菊明%潘长玉
遲鋮%金苗苗%母義明%汪保安%馬曉莉%賈賀堂%楊麗娟%竇京濤%陸菊明%潘長玉
지성%금묘묘%모의명%왕보안%마효리%가하당%양려연%두경도%륙국명%반장옥
胰岛素敏感性%大黄酸%脂肪组织%过氧化物酶体增殖物激活受体%葡萄糖转运蛋白
胰島素敏感性%大黃痠%脂肪組織%過氧化物酶體增殖物激活受體%葡萄糖轉運蛋白
이도소민감성%대황산%지방조직%과양화물매체증식물격활수체%포도당전운단백
Glucose tronoporter
目的 探讨大黄酸改善糖尿病大鼠脂肪组织胰岛素敏感性及降血糖的作用机制.方法 雄性Wistar大鼠随机分为对照组(NC,n=15)及糖尿病造模组(DM,n=40),DM组成模后随机分为糖尿病模型组(DM-C,n=15)和糖尿病大黄酸治疗组(DM-T,n=15).DM-T组予大黄酸100 mg·kg-1·d-1灌胃11周.实验末检测各组大鼠FBG、FIns、TG、TC、胰岛素敏感指数(ISI)、过氧化物酶体增殖物激活受体γ(PPAR-γ)及葡萄糖转运蛋白4(GluT-4)在脂肪组织的表达水平.结果 17周末DM-C组较NC组FBG、TG明显升高,ISI明显降低,脂肪组织PPAR-γ及GluT-4蛋白表达明显降低;DM-T组较DM-C组FBG明显降低;ISI明显升高;脂肪组织PPAR-γ蛋白表达明显升高,积分光密度有统计学差异; GluT-4蛋白表达明显升高,积分密度(ID)有统计学差异.结论 大黄酸可上调糖尿病大鼠脂肪组织PPAR-γ及GluT-4蛋白表达,降血糖并改善胰岛素敏感性.
目的 探討大黃痠改善糖尿病大鼠脂肪組織胰島素敏感性及降血糖的作用機製.方法 雄性Wistar大鼠隨機分為對照組(NC,n=15)及糖尿病造模組(DM,n=40),DM組成模後隨機分為糖尿病模型組(DM-C,n=15)和糖尿病大黃痠治療組(DM-T,n=15).DM-T組予大黃痠100 mg·kg-1·d-1灌胃11週.實驗末檢測各組大鼠FBG、FIns、TG、TC、胰島素敏感指數(ISI)、過氧化物酶體增殖物激活受體γ(PPAR-γ)及葡萄糖轉運蛋白4(GluT-4)在脂肪組織的錶達水平.結果 17週末DM-C組較NC組FBG、TG明顯升高,ISI明顯降低,脂肪組織PPAR-γ及GluT-4蛋白錶達明顯降低;DM-T組較DM-C組FBG明顯降低;ISI明顯升高;脂肪組織PPAR-γ蛋白錶達明顯升高,積分光密度有統計學差異; GluT-4蛋白錶達明顯升高,積分密度(ID)有統計學差異.結論 大黃痠可上調糖尿病大鼠脂肪組織PPAR-γ及GluT-4蛋白錶達,降血糖併改善胰島素敏感性.
목적 탐토대황산개선당뇨병대서지방조직이도소민감성급강혈당적작용궤제.방법 웅성Wistar대서수궤분위대조조(NC,n=15)급당뇨병조모조(DM,n=40),DM조성모후수궤분위당뇨병모형조(DM-C,n=15)화당뇨병대황산치료조(DM-T,n=15).DM-T조여대황산100 mg·kg-1·d-1관위11주.실험말검측각조대서FBG、FIns、TG、TC、이도소민감지수(ISI)、과양화물매체증식물격활수체γ(PPAR-γ)급포도당전운단백4(GluT-4)재지방조직적표체수평.결과 17주말DM-C조교NC조FBG、TG명현승고,ISI명현강저,지방조직PPAR-γ급GluT-4단백표체명현강저;DM-T조교DM-C조FBG명현강저;ISI명현승고;지방조직PPAR-γ단백표체명현승고,적분광밀도유통계학차이; GluT-4단백표체명현승고,적분밀도(ID)유통계학차이.결론 대황산가상조당뇨병대서지방조직PPAR-γ급GluT-4단백표체,강혈당병개선이도소민감성.
Objective To investigate the mechanism for rhein to improve the insulin sensitivity in diabetes mellitus. Methods Male Wistar rats were randomly divided into normal control group (NC,n=15) and diabetic group (DM,n=40) induced by STZ and high-fat diet.Diabetic rats were randomly divided into diabetic control group (DM-C,n=15) and diabetic group treated with rhein (DM-T,n=15).Then DM-T group was given intragastric administration with rhein at the dose of 100 mg/ (kg-1·d-1) for 11 weeks.FBG,fasting serum insulin (FIns),triglyceride (TG),total cholesterol (TC),insulin sensitivity index (ISI) and other biochemical parameters in each group were measured.The protein expressions of PPAR-γ and GluT-4 were determined by immunohistochemistry and Western blot. Results At the end of the 17th week,compared with NC group,DM-C group showed higher FBG and TG,lower ISI,lower protein expressions of PPAR-γ and GluT-4 in the adipose tissue.Compared with DM-C group,DM-T group showed lower FBG [(15.73±3.08 vs 22.57±3.23) mmol/l,P<0.01],higher ISI (-4.97±0.29 vs -5.46±0.61,P<0.05).Integrated optical density (IOD) showed higher protein expression of PPAR-γ of DM-T group in adipose tissue as compared with DM-C group [13613.35 (7293.85~17198.73) vs 4750.70 (3346.63~10390.63),P<0.01].Integrated density (ID) showed higher protein expression of GluT-4 of DM-T group in adipose tissue as compared with DM-C group [1.157 (0.921~1.953) vs 0.828 (0.621~1.010),P<0.01]. Conclusions Rhein increases the protein expressions of PPAR-γ and GluT-4 in adipose tissue,decreases FBG and improves insulin sensitivity of diabetic rats