浙江预防医学
浙江預防醫學
절강예방의학
ZHEJIANG JOURNAL OF PREVENTIVE MEDICINE
2009年
7期
3-6
,共4页
张淑芝%姚耿东%鲁德强%姜槐%许正平
張淑芝%姚耿東%魯德彊%薑槐%許正平
장숙지%요경동%로덕강%강괴%허정평
射频电磁场%神经元%基因芯片%基因表达谱
射頻電磁場%神經元%基因芯片%基因錶達譜
사빈전자장%신경원%기인심편%기인표체보
Radiofrequency electromagnetic fields%Neuron%Gene chip%Gene expression profile
目的 了解1.8 GHz移动电话射频电磁场(RF EMF)对大鼠神经细胞基因表达谱的影响,筛选射频电磁场反应基因.方法 将原代培养的大鼠神经细随机分为实验组(辐照组)和对照组(假辐照组),经1.8 GHz射频电磁场,SAR为2 W·kg-1,间断辐照24 h(5 min开/10 min关)后,即刻抽提两组细胞的总RNA,用Affymetrix公司的Rat Neurobiology U34 array基因芯片筛选其差异表达基因,寻找可能的RF EMF反应候选基因.结果 在1200个候选基因中,筛选出34个差异表达基因,其中上调基因24个,下调基因10个.差异表达基因与多种细胞功能相关(细胞骨架、信号通路、细胞代谢等).这些基因的变化均小于2倍,但具有统计学意义(P<0.01).结论 1.8 GHz射频辐射影响大鼠神经元多个基因的表达,某些基因的改变提示射频辐射对神经元可能产生不良影响.
目的 瞭解1.8 GHz移動電話射頻電磁場(RF EMF)對大鼠神經細胞基因錶達譜的影響,篩選射頻電磁場反應基因.方法 將原代培養的大鼠神經細隨機分為實驗組(輻照組)和對照組(假輻照組),經1.8 GHz射頻電磁場,SAR為2 W·kg-1,間斷輻照24 h(5 min開/10 min關)後,即刻抽提兩組細胞的總RNA,用Affymetrix公司的Rat Neurobiology U34 array基因芯片篩選其差異錶達基因,尋找可能的RF EMF反應候選基因.結果 在1200箇候選基因中,篩選齣34箇差異錶達基因,其中上調基因24箇,下調基因10箇.差異錶達基因與多種細胞功能相關(細胞骨架、信號通路、細胞代謝等).這些基因的變化均小于2倍,但具有統計學意義(P<0.01).結論 1.8 GHz射頻輻射影響大鼠神經元多箇基因的錶達,某些基因的改變提示射頻輻射對神經元可能產生不良影響.
목적 료해1.8 GHz이동전화사빈전자장(RF EMF)대대서신경세포기인표체보적영향,사선사빈전자장반응기인.방법 장원대배양적대서신경세수궤분위실험조(복조조)화대조조(가복조조),경1.8 GHz사빈전자장,SAR위2 W·kg-1,간단복조24 h(5 min개/10 min관)후,즉각추제량조세포적총RNA,용Affymetrix공사적Rat Neurobiology U34 array기인심편사선기차이표체기인,심조가능적RF EMF반응후선기인.결과 재1200개후선기인중,사선출34개차이표체기인,기중상조기인24개,하조기인10개.차이표체기인여다충세포공능상관(세포골가、신호통로、세포대사등).저사기인적변화균소우2배,단구유통계학의의(P<0.01).결론 1.8 GHz사빈복사영향대서신경원다개기인적표체,모사기인적개변제시사빈복사대신경원가능산생불량영향.
Objective To investigate the changes of gene expression in rat neurons induced by 1.8 GHz radiofrequency electromagnetic fields (RF EMF) and to screen RF EMF-responsive genes. Methods The rat primary cultured neuronal cells were divided into two groups, the radiation group and control group, from which the total RNA was extracted immediately and purified after intermittently (5min on/10min off) exposed or U34 array was applied to detect the changes of gene expression in rat neurons. Results Among 1200 candidate genes, 24 up-regulated and 10 down-regulated genes which are associated with multiple cellular functions (cytoskeleton, signal transduction pathway, metabolism, etc.) after functional classification were found by using Affymetrix microarray suite software 5.0. Although the changes in gene expression were less than 2 folds, they had statistical significance (P<0.01). Conclusion RF radiation of 1.8GHz induce the changes of many genes transcription in rat neurons, some of which indicate the negative effects of RF radiation on neurons.