CAJ | 학술논문

目的检测良性前列腺增生(BPH)患者组织中蛋白表达的特点,了解有无与BPH相关的特异性蛋白表达.方法通过双向电泳方法所发现的检测BPH组织特异性蛋白表达,αs1-Casein进行定量免疫组化研究来验证该蛋白表达的特异性.结果通过对正常前列腺组织,BPH及前列腺癌组织进行免疫组织化学染色,发现αs1-Casein在90%(20/22)的BPH组织中呈弱到强表达,而在正常前列腺组织中未见表达(0/10),在前列腺癌组织中的表达也不足10%(3/30).结论 αs1-Casein可能是一种BPH诊断的生物标记物.
목적 검측량성전렬선증생(BPH)환자조직중단백표체적특점,료해유무여BPH상관적특이성단백표체.방법 통과쌍향전영방법 소발현적검측BPH조직특이성단백표체,αs1-Casein진행정량면역조화연구래험증해단백표체적특이성.결과 통과대정상전렬선조직,BPH급전렬선암조직진행면역조직화학염색,발현αs1-Casein재90%(20/22)적BPH조직중정약도강표체,이재정상전렬선조직중미견표체(0/10),재전렬선암조직중적표체야불족10%(3/30).결론 αs1-Casein가능시일충BPH진단적생물표기물.
Objective To analyze the characteristic of protein profile of benign prostatic hyperplasia (BPH) tissue and to further find the special proteins associated with BPH. Methods Protein profiles of human expressed prostatic secretion (EPS) samples obtained from BPH patients and normal males were comparatively analyzed by two-dimensional gel electrophoresis. In the resulting electrophoretograms, several differentially expressed proteins were identified. In this study, a distinct expressed protein αs1-casein was validated in BPH. Results Immunohistochemistry analysis showed that αs1-casein was mainly distributed in the epithelium of hyperplastic prostate. The level of the exression of αs1-casein in the tissue of BPH was found to be higher than that in prostatic carcinoma; whereas no asl-casein was detected in normal tissue.Western blotting analysis was also revealed same expression pattern of asl-casein was detected in BPH-EPS and BPH-free EPS. Conclusion asl-casein might be associated with BPH development and be used as a potential biological marker for the diagnosis of BPH.