CAJ | 학술논문

为了研究细菌纤维素(BC)植入皮下组织后对其周围组织细胞产生的影响,以及由于周围细胞的长入和组织液中相关成分的直接接触,使材料在体内复杂环境下所发生的物理结构变化,以组织工程用支架基体材料细菌纤维素为研究对象,对这种材料的体内降解行为和体内组织相容性进行研究.选取成熟的Wistar大鼠,采取背部皮下植入的方式,将BC植入一定时间后,对取出的BC清洗干净后进行材料学检测分析,对植入部位的组织进行透射电镜观察分析.对取出的材料进行结构和形貌表征可知,随着植入时间的延长,在细胞和组织液的综合作用下,BC分子链中部分C-O-C键断裂.分子间结合力降低,结晶度逐渐下降.因此,BC在体内复杂环境的影响下会发生轻微降解.BC与皮下组织有较好的生物相容性,材料周围组织细胞状态良好,能长入材料30μm处的空间网络结构中.并且伴有血管生成.
위료연구세균섬유소(BC)식입피하조직후대기주위조직세포산생적영향,이급유우주위세포적장입화조직액중상관성분적직접접촉,사재료재체내복잡배경하소발생적물리결구변화,이조직공정용지가기체재료세균섬유소위연구대상,대저충재료적체내강해행위화체내조직상용성진행연구.선취성숙적Wistar대서,채취배부피하식입적방식,장BC식입일정시간후,대취출적BC청세간정후진행재료학검측분석,대식입부위적조직진행투사전경관찰분석.대취출적재료진행결구화형모표정가지,수착식입시간적연장,재세포화조직액적종합작용하,BC분자련중부분C-O-C건단렬.분자간결합력강저,결정도축점하강.인차,BC재체내복잡배경적영향하회발생경미강해.BC여피하조직유교호적생물상용성,재료주위조직세포상태량호,능장입재료30μm처적공간망락결구중.병차반유혈관생성.
Bacterial Cellulose (BC) used in tissue engineering is studied in this paper, with the focus on the effects of bacterial cellulose on rhagiocrine cell and the structural changes of materials owing to tissue cells entering. To study the in vivo degradation and histocompatibility of BC, BC with the size of 5 mm×10 mm×1 mm was implanted in the subcutaneous tissue back of mature Wistar rats. After a period of time, BC was taken out and cleaned out with Sodium Dodecyl Sulfate (SDS), Phosphate Buffer Solution (PBS) and distilled water. And then the net BC taken out in different times was detected and analyzed by XRD, SEM and FTIR. At the same time, the corresponding subcutaneous tissue was fixed and observed under Transmission Electron Microscope (TEM). The interface of materials and organization tissue was observed by TEM to investigate the condition of histiocyte growing into 3D network of materials. The materials were taken out from the tissue after BC was implanted for several months. It is found that the crystallinity of BC is stepped down and C-O bond characteristic absorption peak broadened. Moreover, calcium phosphate salts were found deposited on the nano-fibril of BC after implanted for 6 months. Bacterial cellulose had good histocompatibility with subcutaneous tissue of back. Fibroblasts as well as blood vessel could migrate up to 30 μm into the fibrous network after 6 months of implant.