去甲氯胺酮半抗原及其全抗原的合成与鉴定
거갑록알동반항원급기전항원적합성여감정
Synthesis and Identification of Hapten and Complete Antigen of Norketa mine
  • 万方数据
    分析化学 분석화학
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
    2010年 1期 109-112 ,共4页

    杨婷婷%赵春城%沈笑平%谈伯昌%邹盘坤%王怀明%刘凌云%韦超%魏万里%何金海%吴杰%张东升%赵晓联

    양정정%조춘성%침소평%담백창%추반곤%왕부명%류릉운%위초%위만리%하금해%오걸%장동승%조효련

    去甲氯胺酮%半抗原%人工抗原%合成%鉴定 去甲氯胺酮%半抗原%人工抗原%閤成%鑒定
    거갑록알동%반항원%인공항원%합성%감정
    Norketamine%Hapten%Artificial antigen%Identification
    在低温条件下,去甲氯胺酮与琥珀醛酸反应,合成了半抗原羧基-去甲氯胺酮,电喷雾质谱鉴定结果表明,目标半抗原合成成功;通过碳二亚胺法将半抗原与载体蛋白偶联制备人工抗原,红外光谱法鉴定结果表明,人工抗原合成成功,基质辅助激光解析电离飞行时间质谱鉴定表明去甲氯胺酮半抗原与牛血清白蛋白的偶联比为11:1.经动物免疫,获得高效价特异性多克隆抗体,抗血清效价可达5.12×10~4.
    재저온조건하,거갑록알동여호박철산반응,합성료반항원최기-거갑록알동,전분무질보감정결과표명,목표반항원합성성공;통과탄이아알법장반항원여재체단백우련제비인공항원,홍외광보법감정결과표명,인공항원합성성공,기질보조격광해석전리비행시간질보감정표명거갑록알동반항원여우혈청백단백적우련비위11:1.경동물면역,획득고효개특이성다극륭항체,항혈청효개가체5.12×10~4.
    Under low temperature conditions, the hapten carboxyl-norketamine was synthesized by reacting norketamine and succinaldehyde acid.Identification result using electrospray ionization mass showed the hap ten was successfully synthesized.The artificial antigen confirmed by infrared spectroscopy was developed by conjugating hapten to carrier proteins with carbodiimide(EDC) method.Matrix-assisted laser desorption ioni zation time of flight mass spectrometry(MALDI-TOF-MS) showed that the ratio of hapten to BSA was 11:1.The antibody with high titer(5.12 × 10~4) was produced after immuning to rabbits.
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