上海交通大学学报（医学版）
上海交通大學學報（醫學版）
상해교통대학학보（의학판）
JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY(MEDICAL SCIENCE)
2010年
2期
154-157
,共4页
子(癎)前期%细胞滋养细胞%浸润%基质金属蛋白酶%纤溶酶原激活物抑制剂-1
子(癎)前期%細胞滋養細胞%浸潤%基質金屬蛋白酶%纖溶酶原激活物抑製劑-1
자(간)전기%세포자양세포%침윤%기질금속단백매%섬용매원격활물억제제-1
preeclampsia%cytotrophoblast%invasion%matrix metalloproteinases%plasminogen activator inhibitor-1
目的 探讨子(癎)前期患者血清对人早孕细胞滋养细胞活力和浸润功能的影响.方法 采用组织块贴壁法培养人早孕期(孕6-8周)细胞滋养细胞,传代后待细胞长满至70%-80%,分别加入正常孕妇血清(正常组)和子(癎)前期患者血清(子(癎)前期组)培养24 h;CCK-8法测定细胞活力;Transwell技术检测细胞滋养细胞的浸润功能;RT-PCR法检测滋养细胞MMP-2、MMP-9和PAI-1 mRNA的表达.结果 子(癎)前期组细胞滋养细胞活力和浸润能力均低于正常组(P<0.05和P<0.01).与正常组比较,子(癎)前期组细胞滋养细胞MMP-2和MMP-9 mRNA的表达明显下降,而PAI-1 mRNA的表达明显升高,差异均有统计学意义(P<0.01).正常组和子(癎)前期组中,MMP-2 mRNA与PAI-1 mRNA的表达均呈负相关(r=-0.985,P<0.01;r=-0.933,P<0.05);MMP-9 mRNA与PAI-1 mRNA的表达无相关性.结论 子(癎)前期患者血清可能通过调节细胞滋养细胞中MMP-2、MMP-9和PAI-1的表达而影响细胞的浸润能力.
目的 探討子(癎)前期患者血清對人早孕細胞滋養細胞活力和浸潤功能的影響.方法 採用組織塊貼壁法培養人早孕期(孕6-8週)細胞滋養細胞,傳代後待細胞長滿至70%-80%,分彆加入正常孕婦血清(正常組)和子(癎)前期患者血清(子(癎)前期組)培養24 h;CCK-8法測定細胞活力;Transwell技術檢測細胞滋養細胞的浸潤功能;RT-PCR法檢測滋養細胞MMP-2、MMP-9和PAI-1 mRNA的錶達.結果 子(癎)前期組細胞滋養細胞活力和浸潤能力均低于正常組(P<0.05和P<0.01).與正常組比較,子(癎)前期組細胞滋養細胞MMP-2和MMP-9 mRNA的錶達明顯下降,而PAI-1 mRNA的錶達明顯升高,差異均有統計學意義(P<0.01).正常組和子(癎)前期組中,MMP-2 mRNA與PAI-1 mRNA的錶達均呈負相關(r=-0.985,P<0.01;r=-0.933,P<0.05);MMP-9 mRNA與PAI-1 mRNA的錶達無相關性.結論 子(癎)前期患者血清可能通過調節細胞滋養細胞中MMP-2、MMP-9和PAI-1的錶達而影響細胞的浸潤能力.
목적 탐토자(간)전기환자혈청대인조잉세포자양세포활력화침윤공능적영향.방법 채용조직괴첩벽법배양인조잉기(잉6-8주)세포자양세포,전대후대세포장만지70%-80%,분별가입정상잉부혈청(정상조)화자(간)전기환자혈청(자(간)전기조)배양24 h;CCK-8법측정세포활력;Transwell기술검측세포자양세포적침윤공능;RT-PCR법검측자양세포MMP-2、MMP-9화PAI-1 mRNA적표체.결과 자(간)전기조세포자양세포활력화침윤능력균저우정상조(P<0.05화P<0.01).여정상조비교,자(간)전기조세포자양세포MMP-2화MMP-9 mRNA적표체명현하강,이PAI-1 mRNA적표체명현승고,차이균유통계학의의(P<0.01).정상조화자(간)전기조중,MMP-2 mRNA여PAI-1 mRNA적표체균정부상관(r=-0.985,P<0.01;r=-0.933,P<0.05);MMP-9 mRNA여PAI-1 mRNA적표체무상관성.결론 자(간)전기환자혈청가능통과조절세포자양세포중MMP-2、MMP-9화PAI-1적표체이영향세포적침윤능력.
Objective To investigate the effects of preeclampsia serum on activity and invasive ability of cultured cytotrophoblasts of first trimester of pregnancy. Methods Cytotrophoblasts of normal 6 to 8-week pregnancy were cultured by tissue explants adherent method,and were incubated with serum of women with normal pregnancy(normal group)and preeclampsia(preeclampsia group),respectively for 24 h.The activity of cytotrophoblasts was examined by CCK-8,invasive ability was determined by Transwell invasion assay,and expression of MMP-2,MMP-9 and PAI-1 mRNA was detected by reverse transcription-polymerase chain reaction(RT-PCR). Results The activity and invasive ability of cytotrophoblasts in preeclampsia group were lower than those in normal group(P<0.05,P<0.01).Compared with normal group,the expression of MMP-2 and MMP-9 mRNA of cytotrophoblasts was significantly lower(P<0.01),and the expression of PAI-1 mRNA was significantly higher(P<0.01).In both groups,the expression of MMP-2 mRNA was negatively related to that of PAI-1 mRNA(r=-0.985,P<0.01;r=-0.933,P<0.05),while there was no correlationship between the expression of MMP-9 mRNA and that of PAI-1 mRNA. Conclusion The preeclampsia serum may affect the invasive ability of cytotrophoblasts by regulating the expression of MMP-2,MMP-9 and PAI-1.
