中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2009年
2期
210-213
,共4页
一氧化氮合酶/拮抗剂和抑制剂/药理学%胎儿窘迫/病理生理学%记忆/药物作用%突触蛋白类/药物作用
一氧化氮閤酶/拮抗劑和抑製劑/藥理學%胎兒窘迫/病理生理學%記憶/藥物作用%突觸蛋白類/藥物作用
일양화담합매/길항제화억제제/약이학%태인군박/병리생이학%기억/약물작용%돌촉단백류/약물작용
Nitric-oxide synthase/AI/PD%Fetal distress/PP%Memory/DE%Synapsins/DE
目的 研究一氧化氮合酶抑制剂对宫内窘迫胎鼠幼年学习记忆能力、海马突触素的影响.方法 (1)建立胎鼠宫内窘迫模型;(2)分为正常组(Nor)、缺血再灌注组(I/R)、治疗组(NOSI).缺血前1.5 h注射左旋硝基精氨酸(L-NNA),然后于术前即刻腹腔内注射氨基胍(AG)500 mg/kg,将正常组、缺血再灌注组和治疗组中的胎鼠继续妊娠至孕19 d,以剖宫产娩出,饲养至40 d);(3)以Morris水迷宫检测幼鼠学习能力后检测海马中突触素的表达情况.结果 各组幼鼠历时5 d训练,共8个时间段;与Nor、NOSI组比较,I/R组平均潜伏期延长(P<0.05).NOSI组与Nor组比较,潜伏期延长,但差异无统计学意义(P>0.05);幼鼠在第5天进行的跨平台次数,I/R组较Nor组、NOSI组明显减少(P<0.05);而NOSI组与Nor组比较,差异无统计学意义(P>0.05).突触素(Syp)的表达:3组的海马均有阳性产物产生,I/R组的COD值较正常组及NOSI组的COD值高,差异有统计学意义(P<0.05);正常组与NOSI组的COD值差异无统计学意义(P>0.05).结论 宫内窘迫对胎鼠脑组织可能造成长期的影响;在宫内窘迫时及时予以NOSI,可能会减轻胎鼠脑损害,改善仔鼠的智能.
目的 研究一氧化氮閤酶抑製劑對宮內窘迫胎鼠幼年學習記憶能力、海馬突觸素的影響.方法 (1)建立胎鼠宮內窘迫模型;(2)分為正常組(Nor)、缺血再灌註組(I/R)、治療組(NOSI).缺血前1.5 h註射左鏇硝基精氨痠(L-NNA),然後于術前即刻腹腔內註射氨基胍(AG)500 mg/kg,將正常組、缺血再灌註組和治療組中的胎鼠繼續妊娠至孕19 d,以剖宮產娩齣,飼養至40 d);(3)以Morris水迷宮檢測幼鼠學習能力後檢測海馬中突觸素的錶達情況.結果 各組幼鼠歷時5 d訓練,共8箇時間段;與Nor、NOSI組比較,I/R組平均潛伏期延長(P<0.05).NOSI組與Nor組比較,潛伏期延長,但差異無統計學意義(P>0.05);幼鼠在第5天進行的跨平檯次數,I/R組較Nor組、NOSI組明顯減少(P<0.05);而NOSI組與Nor組比較,差異無統計學意義(P>0.05).突觸素(Syp)的錶達:3組的海馬均有暘性產物產生,I/R組的COD值較正常組及NOSI組的COD值高,差異有統計學意義(P<0.05);正常組與NOSI組的COD值差異無統計學意義(P>0.05).結論 宮內窘迫對胎鼠腦組織可能造成長期的影響;在宮內窘迫時及時予以NOSI,可能會減輕胎鼠腦損害,改善仔鼠的智能.
목적 연구일양화담합매억제제대궁내군박태서유년학습기억능력、해마돌촉소적영향.방법 (1)건립태서궁내군박모형;(2)분위정상조(Nor)、결혈재관주조(I/R)、치료조(NOSI).결혈전1.5 h주사좌선초기정안산(L-NNA),연후우술전즉각복강내주사안기고(AG)500 mg/kg,장정상조、결혈재관주조화치료조중적태서계속임신지잉19 d,이부궁산면출,사양지40 d);(3)이Morris수미궁검측유서학습능력후검측해마중돌촉소적표체정황.결과 각조유서력시5 d훈련,공8개시간단;여Nor、NOSI조비교,I/R조평균잠복기연장(P<0.05).NOSI조여Nor조비교,잠복기연장,단차이무통계학의의(P>0.05);유서재제5천진행적과평태차수,I/R조교Nor조、NOSI조명현감소(P<0.05);이NOSI조여Nor조비교,차이무통계학의의(P>0.05).돌촉소(Syp)적표체:3조적해마균유양성산물산생,I/R조적COD치교정상조급NOSI조적COD치고,차이유통계학의의(P<0.05);정상조여NOSI조적COD치차이무통계학의의(P>0.05).결론 궁내군박대태서뇌조직가능조성장기적영향;재궁내군박시급시여이NOSI,가능회감경태서뇌손해,개선자서적지능.
Objective To investigate whether intrauterine hypoxia and ischemia can produce long-time effects or NOSI can prevent these damages. Methods Fetal rat intrauterine distress model was constructed. The rats were divided into the normal group, hypoxia and ischemia reperfnsion group and treatment group. Pupa were given to surrogate mothers and the ability of learning and memory at 40 day of age after delivery were examined. Then the water maze test was performed to detect the space learning ability and memory function of rats, and the changing of synaptophysin levels in hippocampns were detected by immunohistochemical staining. Result Behavioral results show that fetal distress produces cognitive impairment demonstrated by Morris water maze performance including a higher escape latency score and a de-creased cross platform time. The COD of Syp positive immunoreactive product in hippocampus were less decreased than that in the normal group or NOSI group. But the behavioral results and the COD of synaptophysin had no difference between normal group and NOSI group. Conclusions Fetal distress produced cognitive impairment and led to the decreasing of synaptophysin in hippocampns. Effective measure can relieve these damages.