国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2009年
2期
11-14
,共4页
SDF-1α/CXCR4%ox-LDL%趋化%单核细胞
SDF-1α/CXCR4%ox-LDL%趨化%單覈細胞
SDF-1α/CXCR4%ox-LDL%추화%단핵세포
SDF-1α/CXCR%ox-LDL%Chemotaxis%Monocyte
目的 探讨基质细胞衍生因子(SDF-1α)对单核细胞的趋化作用,ox-LDL对SDF-1α趋化单核细胞的影响及其对THP-1细胞表达CXCR4的影响.方法 用Transwell迁移实验来研究SDF-1α对单核细胞的趋化作用,将单核细胞或经ox-LDL处理的单核细胞加入上室,在下室加入SDF-1α孵育10h后计数各组下室迁移的细胞数.RT-PCR检测CXCR4表达变化,Western blot检测其蛋白表达变化,观察ox-LDL对THP-1细胞CXCR4表达的剂量和时间效应.结果 SDF-1α呈浓度依赖性诱导单核细胞的迁移,加入CXCR4抗体可明显抑制这种作用.经不同浓度ox-LDL处理48h后的THP-1细胞再用10 ng/mlSDF-1α进行趋化时,结果发现ox-LDL呈浓度依赖性的增加单核细胞的迁移,50 μg/ml ox-LDL组所迁移的细胞数是对照组的11倍.THP-1细胞有基础水平的CXCR4表达,50 μg/ml ox-LDL可使CXCR4的表达上调4-5倍.CXCR4上调最早在6h内发生,12h达高峰.结论 SDF-1α/CXCR4参与趋化单核细胞迁移,其作用被ox-LDL加强;ox-LDL上调CXCR4表达.
目的 探討基質細胞衍生因子(SDF-1α)對單覈細胞的趨化作用,ox-LDL對SDF-1α趨化單覈細胞的影響及其對THP-1細胞錶達CXCR4的影響.方法 用Transwell遷移實驗來研究SDF-1α對單覈細胞的趨化作用,將單覈細胞或經ox-LDL處理的單覈細胞加入上室,在下室加入SDF-1α孵育10h後計數各組下室遷移的細胞數.RT-PCR檢測CXCR4錶達變化,Western blot檢測其蛋白錶達變化,觀察ox-LDL對THP-1細胞CXCR4錶達的劑量和時間效應.結果 SDF-1α呈濃度依賴性誘導單覈細胞的遷移,加入CXCR4抗體可明顯抑製這種作用.經不同濃度ox-LDL處理48h後的THP-1細胞再用10 ng/mlSDF-1α進行趨化時,結果髮現ox-LDL呈濃度依賴性的增加單覈細胞的遷移,50 μg/ml ox-LDL組所遷移的細胞數是對照組的11倍.THP-1細胞有基礎水平的CXCR4錶達,50 μg/ml ox-LDL可使CXCR4的錶達上調4-5倍.CXCR4上調最早在6h內髮生,12h達高峰.結論 SDF-1α/CXCR4參與趨化單覈細胞遷移,其作用被ox-LDL加彊;ox-LDL上調CXCR4錶達.
목적 탐토기질세포연생인자(SDF-1α)대단핵세포적추화작용,ox-LDL대SDF-1α추화단핵세포적영향급기대THP-1세포표체CXCR4적영향.방법 용Transwell천이실험래연구SDF-1α대단핵세포적추화작용,장단핵세포혹경ox-LDL처리적단핵세포가입상실,재하실가입SDF-1α부육10h후계수각조하실천이적세포수.RT-PCR검측CXCR4표체변화,Western blot검측기단백표체변화,관찰ox-LDL대THP-1세포CXCR4표체적제량화시간효응.결과 SDF-1α정농도의뢰성유도단핵세포적천이,가입CXCR4항체가명현억제저충작용.경불동농도ox-LDL처리48h후적THP-1세포재용10 ng/mlSDF-1α진행추화시,결과발현ox-LDL정농도의뢰성적증가단핵세포적천이,50 μg/ml ox-LDL조소천이적세포수시대조조적11배.THP-1세포유기출수평적CXCR4표체,50 μg/ml ox-LDL가사CXCR4적표체상조4-5배.CXCR4상조최조재6h내발생,12h체고봉.결론 SDF-1α/CXCR4삼여추화단핵세포천이,기작용피ox-LDL가강;ox-LDL상조CXCR4표체.
Objective To explore the effect of SDF-1 α /CXCR4 on THP-1 cells chemotaxis and the influence of OX- LDL on THP-1 cells migration esplsed to SDP-1 α,the effect of OX-LDL in the expression of CXCR4 in THP-1 cells.THP-1 cells chemotaxis was examined with transwell permeable supports.THP-1 cells with or withort pretreatde with OX-LDL were input in upper chambers,SDF-1αwas added in low chambers.Number of migrated cells in the low chamber was counted after incubation for 10h.CXCR4 mRNA and protein was revealed by RT-PCR and Western blkt respectively in THP-1 cells incubated with different concentrations/time of OX-LDL.Results SDF-1αinduced a pronounced migration of input cells in a concentration-related manner,but this effect was obviously inhibited by the antibody to CXXR4 was constitutionally expressde in THP-1 cells.Comparing with control group,a foru-to fivefold induction of CXCR4 occurred in THP-1 treated with 50 üg/ml OX-LDL.Upregulation of CXCR4 was within 6h,peaked at 12h then followed with a decline,mirroring the expression pattern in macrophage.Conclusions SDF-1 α/CXCR4 is implicated in THP-1 cells chemotaxis and chemotactic response of monocyte to SDF-1 α is enhanced by Ox-LDL; OX-LDL up-regulates CXCR4 expression.
