CAJ | 학술논문

目的探讨Tspan 8基因对人肝癌细胞侵袭与转移的影响.方法应用RT-PCR与Western印迹检测不同转移潜能肝癌细胞及组织中Tspan 8的表达；应用shRNA-Tspan 8(LV/GFP/Tspan 8)下调Tspan 8表达；MTT法检测细胞增殖变化；Western印迹检测基质金属蛋白酶12表达改变；最后应用Transwell检测细胞侵袭能力变化.结果高表达Tspan 8见于高转移潜能的肝癌细胞,Tspan 8在肝癌组织中表达明显高于相应癌旁组织.感染LV/GFP/Tspan 8、LV/GFP后,LV/GFP/Tspan 8感染肝癌细胞的Tspan 8表达显著下调(P＜0.01)；下调Tspan 8后,细胞增殖反应变化不明显(24 h,48 h与72 h,P＞0.05)；但MMP-12表达抑制明显(P＜0.01),细胞侵袭能力明显下降.结论下调Tspan 8基因的表达对肝癌侵袭与转移能力有明显抑制.
목적 탐토Tspan 8기인대인간암세포침습여전이적영향.방법 응용RT-PCR여Western인적검측불동전이잠능간암세포급조직중Tspan 8적표체；응용shRNA-Tspan 8(LV/GFP/Tspan 8)하조Tspan 8표체；MTT법검측세포증식변화；Western인적검측기질금속단백매12표체개변；최후응용Transwell검측세포침습능력변화.결과 고표체Tspan 8견우고전이잠능적간암세포,Tspan 8재간암조직중표체명현고우상응암방조직.감염LV/GFP/Tspan 8、LV/GFP후,LV/GFP/Tspan 8감염간암세포적Tspan 8표체현저하조(P＜0.01)；하조Tspan 8후,세포증식반응변화불명현(24 h,48 h여72 h,P＞0.05)；단MMP-12표체억제명현(P＜0.01),세포침습능력명현하강.결론 하조Tspan 8기인적표체대간암침습여전이능력유명현억제.
Objective To study the effect of Tspan 8 on metastasis and invasion of human hepatocellular carcinomas(HCC).Methods RT-PCR and western blot were used to detect the expressions of Tspan 8 in HCC cell lines,HCC and matched nontumorous tissues.The expression of Tspan 8 was then down-regulated by LV/GFP/Tspan 8 in HCC cells.The expressions of Tspan 8 mRNA and protein were determined by RT-PCR and Western blot assay,respectively.The proliferation was examined by MTT,the expression of AMDM12 was assessed by Western blot,and the invasion ability of HCC cells was evaluated by transwells.Results A high level of Tspan 8 was found in high metastatic potential HCC cells,and the expression of Tspan 8 in HCC tissues was much higher than that in the matched nontumorous tissues. Down-regulation of Tspan 8 had no influence on the proliferation of HCC cells (P＞0.05),while it inhibited the expression of ADAM12 and the invasive ability of HCC cells (P＜0.01,P＜0.01 respectively).Conclusion Tspan 8 played an important role in invasion and metastasis of human hepatocellular carcinomas and down-regulation by LV/GFP/Tspan 8 inhibited the invasiveness of human hepatocellular carcinoma cells.