CAJ | 학술논문

目的研究高游离脂肪酸(FFA)血症对胰岛β细胞功能的影响.方法正常雄性SD大鼠随机分为3组:对照组输注生理盐水,FFA组输注脂肪乳,氮乙酰半胱氨酸(NAC)干预组在输注脂肪乳的同时加用NAC.持续输液2～4天,行静脉葡萄糖耐最试验(IVGTY)和胰腺组织表面灌注试验评价在体及离体情况下胰岛β细胞胰岛素分泌功能.结果胰腺组织表面灌注结果显示,FFA组脂肪乳输注2天,基础状态下的胰岛素分泌较对照组增加[(55.5±19.4 vs 27.4±6.7)mIU/L,P<0.01],而葡萄糖刺激的胰岛素分泌(GSIS)与对照组并无差别;延长脂肪乳输注到4天,高FFA刺激基础胰岛素分泌的作用消失,且GSIS明显抑制[(46.8±33.0 vs 214.7±27.4)mIU/L,P<0.05].IVG3T结果亦显示脂肪乳输注4天后胰岛素分泌功能受到抑制.但在脂肪乳输注的同时加用抗氧化剂NAC可使β细胞GSIS峰值部分恢复[(165.4±14.8)mIU/L,P<0.01].结论循环中FFA水平增高可能通过激活氧化应激导致胰岛β细胞功能受损.
목적 연구고유리지방산(FFA)혈증대이도β세포공능적영향.방법 정상웅성SD대서수궤분위3조:대조조수주생리염수,FFA조수주지방유,담을선반광안산(NAC)간예조재수주지방유적동시가용NAC.지속수액2～4천,행정맥포도당내최시험(IVGTY)화이선조직표면관주시험평개재체급리체정황하이도β세포이도소분비공능.결과 이선조직표면관주결과현시,FFA조지방유수주2천,기출상태하적이도소분비교대조조증가[(55.5±19.4 vs 27.4±6.7)mIU/L,P<0.01],이포도당자격적이도소분비(GSIS)여대조조병무차별;연장지방유수주도4천,고FFA자격기출이도소분비적작용소실,차GSIS명현억제[(46.8±33.0 vs 214.7±27.4)mIU/L,P<0.05].IVG3T결과역현시지방유수주4천후이도소분비공능수도억제.단재지방유수주적동시가용항양화제NAC가사β세포GSIS봉치부분회복[(165.4±14.8)mIU/L,P<0.01].결론 순배중FFA수평증고가능통과격활양화응격도치이도β세포공능수손.
Objective To evaluate the effects of elevated circulating free fatty acids (FFA) level on basal and glucose stimulated insulin secretion (GSIS) of islet β-cell and to explore the pathophysiological link between FFA and impaired β-cell dysfunction. Methods Male SD rats underwent infusions with normal saline (C group), intralipid+heparin (FFA group) and N-acetylcysteine+FFA (NAC group) for 2-4 days. Insulin secretion from pancreatic tissues was evaluated during intravenous glucose tolerance test and isolated pancreas perfasion test at the end of 2 and 4 days infusion. Results After 2 days infusion, the basal insulin secretion from isolated perfused pancreas was increased in FFA group [(55.5±19.4 vs 27.4±6.7) mU/L, P<0.01], but the response to 16.7 mmol/L glucose in isolated perfased pancreas was similar in FFA and C groups. The peak value during GSIS was inhibited by 4 days FFA infusion [(46.8±33.0 vs 214.7±27.4)mIU/L,P<0.05]. GSIS was also decreased in FFA group compared with C group in IVGTr. After interfered with NAC, GSIS was partly recovered [(165.4± 14.8)mIU/L, P<0.01]. Conclusion Elevated circulating FFA levels may contribute to the abnormality of pancreatic islet β-cell through oxidative stress.