CAJ | 학술논문

目的研究温石棉(CA)与岩棉(RW)和硅灰石(WS)纤维的细胞毒性.方法将V79细胞分为CA组、RW组及WS组3个实验及1个阴性对照组(200 μl PBS),CA、RW及WS粉尘终质量浓度为100 mg/L,噻唑蓝(MTT)法观察V79细胞存活率,用动力学法测乳酸脱氢酶(LDH)活力,用扫描电子显微镜观察粉尘对V79细胞形态的影响.结果 SiO2均为3种纤维的主要成分,WS中的SiO2含量最高,为50.83%.CA组、RW组、WS的细胞存活率分别为54.5%、64.8%、65.7%,RW组和WS 组的存活率明[显高于CA组,差异有统计学意义(P＜0.01),RW组和WS组的LDH活力[分别为(15.7±0.9)、(12.3±3.7)U/L]明显低于CA组[(20.2±0.9)U/L].差异有统计学意义(P＜0.05).CA组的V79细胞两端堆积大量颗粒状残余体,RW组和WS组V79细胞形态基本正常.结论 RW和WS对V79细胞的毒性低于CA.
목적 연구온석면(CA)여암면(RW)화규회석(WS)섬유적세포독성.방법 장V79세포분위CA조、RW조급WS조3개실험급1개음성대조조(200 μl PBS),CA、RW급WS분진종질량농도위100 mg/L,새서람(MTT)법관찰V79세포존활솔,용동역학법측유산탈경매(LDH)활력,용소묘전자현미경관찰분진대V79세포형태적영향.결과 SiO2균위3충섬유적주요성분,WS중적SiO2함량최고,위50.83%.CA조、RW조、WS적세포존활솔분별위54.5%、64.8%、65.7%,RW조화WS 조적존활솔명[현고우CA조,차이유통계학의의(P＜0.01),RW조화WS조적LDH활력[분별위(15.7±0.9)、(12.3±3.7)U/L]명현저우CA조[(20.2±0.9)U/L].차이유통계학의의(P＜0.05).CA조적V79세포량단퇴적대량과립상잔여체,RW조화WS조V79세포형태기본정상.결론 RW화WS대V79세포적독성저우CA.
Objective To study the cytotoxicity induced by chrysotile asbestos (CA), rock wool (RW)and wollastonite (WS). Methods V79 cells were divided into 4 groups. i.e. CA group, WS group, RW group and control group (200 μ1 PBS). The exposure concentration of dusts was 100 mg/L, The cell viability was detected by MTT and lactate dehydrogenate (LDH) activity assays. The technique of scanning electron microscopy was used to examine the change of V79 cells. Results SiO2 was main constituent for 3 kinds of dusts. In MTT assay, the cell viability of RW and WS groups was 64.8% and 65.7%, respectively, which were significantly higher than that (54.5%) of CA group (P＜0.01). In LDH assay, the LDH activity of RW and WS groups [(15.7±50.9),( 12.3±3.7)U/L, respectively] was significantly lower than that[( 20.2±0.9) U/L]of CA group (P＜0.05). In scanning electron microscopy examination, it was found that the two ends of V79 cells in CA group contained a great deal of fibers remaining bodies, but the V79 cell appearance in RW and WS groups was normal. Conclusion The cytotoxicity induced by RW and WS is significantly lower than that induced by CA for V79 cell.