中华神经科杂志
中華神經科雜誌
중화신경과잡지
Chinese Journal of Neurology
2010年
9期
649-654
,共6页
任传成%杨立刚%靳峥%章军建
任傳成%楊立剛%靳崢%章軍建
임전성%양립강%근쟁%장군건
梗死,大脑中动脉%脑缺血%疾病模型,动物
梗死,大腦中動脈%腦缺血%疾病模型,動物
경사,대뇌중동맥%뇌결혈%질병모형,동물
Infarction,middle cerebral artery%Brain ischemia%Disease models,animal
目的 建立一种稳定可靠的脑缺血研究模型.方法 (1)远端大脑中动脉闭塞(dMCAO)模型的制作:SD大鼠(270～350 g),异氟烷麻醉,永久阻断左侧大脑中动脉远端并夹闭双侧颈总动脉30 min即制作成功dMCAO模型.(2)模型的评定:分别于术后10 h行PET扫描、术后24 h行MRI扫描;术后48 h行TTC染色进行梗死面积的评定;分别于术前,术后1、2、7、10、14、21、30、37、44、51、60 d进行行为学评定;CV染色评定细胞受损情况;并计算死亡率.结果 MRI测定梗死面积为45.30%±2.35%(95%可信限:42.94～47.86),TTC测得梗死面积为43.39%±2.33%(95%可信限:40.94～45.84),PET测得缺血面积为54.50%±3.15%(95%可信限:49.49～59.51).术后60 d CV染色测得梗死面积为30.10%±2.22%(95%可信限:28.05～32.15),行为学评定:基线评分两组无差异,60 d总评分差异有统计学意义,在术后各时间点梗死组与对照组相比差异也有统计学意义.模型制作过程中无个体排除,无死亡.结论 30 min一过性阻断双侧颈总动脉加永久性阻断左侧大脑中动脉远端所产生的dMCAO模型稳定、可靠,适用于脑缺血研究.
目的 建立一種穩定可靠的腦缺血研究模型.方法 (1)遠耑大腦中動脈閉塞(dMCAO)模型的製作:SD大鼠(270～350 g),異氟烷痳醉,永久阻斷左側大腦中動脈遠耑併夾閉雙側頸總動脈30 min即製作成功dMCAO模型.(2)模型的評定:分彆于術後10 h行PET掃描、術後24 h行MRI掃描;術後48 h行TTC染色進行梗死麵積的評定;分彆于術前,術後1、2、7、10、14、21、30、37、44、51、60 d進行行為學評定;CV染色評定細胞受損情況;併計算死亡率.結果 MRI測定梗死麵積為45.30%±2.35%(95%可信限:42.94～47.86),TTC測得梗死麵積為43.39%±2.33%(95%可信限:40.94～45.84),PET測得缺血麵積為54.50%±3.15%(95%可信限:49.49～59.51).術後60 d CV染色測得梗死麵積為30.10%±2.22%(95%可信限:28.05～32.15),行為學評定:基線評分兩組無差異,60 d總評分差異有統計學意義,在術後各時間點梗死組與對照組相比差異也有統計學意義.模型製作過程中無箇體排除,無死亡.結論 30 min一過性阻斷雙側頸總動脈加永久性阻斷左側大腦中動脈遠耑所產生的dMCAO模型穩定、可靠,適用于腦缺血研究.
목적 건립일충은정가고적뇌결혈연구모형.방법 (1)원단대뇌중동맥폐새(dMCAO)모형적제작:SD대서(270～350 g),이불완마취,영구조단좌측대뇌중동맥원단병협폐쌍측경총동맥30 min즉제작성공dMCAO모형.(2)모형적평정:분별우술후10 h행PET소묘、술후24 h행MRI소묘;술후48 h행TTC염색진행경사면적적평정;분별우술전,술후1、2、7、10、14、21、30、37、44、51、60 d진행행위학평정;CV염색평정세포수손정황;병계산사망솔.결과 MRI측정경사면적위45.30%±2.35%(95%가신한:42.94～47.86),TTC측득경사면적위43.39%±2.33%(95%가신한:40.94～45.84),PET측득결혈면적위54.50%±3.15%(95%가신한:49.49～59.51).술후60 d CV염색측득경사면적위30.10%±2.22%(95%가신한:28.05～32.15),행위학평정:기선평분량조무차이,60 d총평분차이유통계학의의,재술후각시간점경사조여대조조상비차이야유통계학의의.모형제작과정중무개체배제,무사망.결론 30 min일과성조단쌍측경총동맥가영구성조단좌측대뇌중동맥원단소산생적dMCAO모형은정、가고,괄용우뇌결혈연구.
Objective To create a stable and reliable model for cerebral ischemia.Methods (1)Distal middle cerebral artery occlusion (dMCAO) model: SD rats of 270-350 g in weight were anesthetized using isoflurane.Both common carotid arteries (CCA) were exposed and occluded for 30 min.Via a bone window between the left eye and ear, the exposed left middle cerebral artery was cauterized and cut during bilateral CCA occlusion.(2) Evaluation of the model: microPET study was performed at 10 h after surgery.microMRI scan were done at 24 h.TTC staining were done at 48 h.Behavioral tests, including vibrissaeelicited forelimb placement test, were done from day 2 to 60 after surgery.Tissue damage was evaluated using cresyl-violet staining Mortality was also observed.Results Infarction areas were 54.50% ± 3.15%(95% CI:49.49-59.51 ) using microPET scanning, 45.30% ± 2.35% (95% CI:42.94-47.86) using microMRI scanning, 43.39% ± 2.33% (95% CI:40.94-45.84)using TTC staining, and 30.10% ±2.22% (95% CI:28.05-32.15) using CV staining.The behavioral test scores were lower in the ischemic group than in the sham control group.This dMCAO model was successfully performed in all rats, and the mortality rate was 0.Conclusions Our results suggest that permanent dMCAO plus bilateral CCA occlusion for 30 min can produce a stable and reliable model suitable for research on cerebral ischemia.
