CAJ | 학술논문

目的探讨大鼠坐骨神经慢性卡压损伤后其卡压神经段内结缔组织生长因子(CTGF)表达变化及其意义. 方法 2010年7月到2010年9月,将50只成年雄性SD大鼠随机分成:A组(假手术组),仅分离暴露坐骨神经；B组(卡压组),采用Mackinnon建立的坐骨神经卡压模型方法对大鼠右侧后腿坐骨神经行硅胶管卡压术.于卡压术后2、4、6、8、10周时间点,随机取A、B组大鼠各5只,取其卡压段坐骨神经行组织形态学、免疫组织化学观察及RT-PCR、Western blot定量测定CTGF及Ⅰ、Ⅲ型胶原蛋白(COL-Ⅰ、Ⅲ)含量. 结果坐骨神经慢性卡压损伤后,有髓神经纤维发生瓦勒变性,胶原纤维增生,CTGF表达上调,并伴有COL-I、Ⅲ表达升高,与假手术组比较差异有统计学意义(P＜0.05).结论慢性卡压损伤可致周围神经发生纤维化病变,CTGF参与了这一病理生理改变过程,提示CTGF在周围神经纤维化过程中具有一定作用.
목적 탐토대서좌골신경만성잡압손상후기잡압신경단내결체조직생장인자(CTGF)표체변화급기의의. 방법 2010년7월도2010년9월,장50지성년웅성SD대서수궤분성:A조(가수술조),부분리폭로좌골신경；B조(잡압조),채용Mackinnon건립적좌골신경잡압모형방법대대서우측후퇴좌골신경행규효관잡압술.우잡압술후2、4、6、8、10주시간점,수궤취A、B조대서각5지,취기잡압단좌골신경행조직형태학、면역조직화학관찰급RT-PCR、Western blot정량측정CTGF급Ⅰ、Ⅲ형효원단백(COL-Ⅰ、Ⅲ)함량. 결과 좌골신경만성잡압손상후,유수신경섬유발생와륵변성,효원섬유증생,CTGF표체상조,병반유COL-I、Ⅲ표체승고,여가수술조비교차이유통계학의의(P＜0.05).결론 만성잡압손상가치주위신경발생섬유화병변,CTGF삼여료저일병리생리개변과정,제시CTGF재주위신경섬유화과정중구유일정작용.
Objective To investigate the effects of connective tissue growth factor (CTGF) on the chronic peripheral nerve compression injury and explore the function of CTGF in peripheral nerve compression injury and repair. Methods From July 2010 to September 2010, fifty aduh male SD rats were randomly divided into group A and B: group A (sham-operated group): only exposed the sciatic nerve; group B (compression group): undergone sciatic nerve entrapment operation on the right hind leg according to the method which Mackinnon adopted when he established the model of chronic sciatic nerve compression.Electron microscopy,immunohistochemistry,RT-PCR and Western-blot were performed to observe the morphological changes of the compressed nerve tissue and to determine the level of CTGF,collagen- Ⅰ,Ⅲ (COL- Ⅰ,Ⅲ),2,4,6,8,10 weeks after the surgery,respectively. Results After sciatic nerve compression,the collagen in nerve increased ; The expression of CTGF and COL- Ⅰ, Ⅲ in sciatic nerve of compressed group increased, which was statistically different compared with the sham-operation group (P ＜ 0.05); In the meanwhile,the contents of CTGF and COL- Ⅰ,Ⅲ were positively correlated in a certain period. Conclusion Peripheral nerve fibrosis can be caused by chronic nerve compression.The expression of COL- Ⅰ,Ⅲ in sciatic nerve increased and CTGF get involved in the pathophysiological process, which suggests that CTGF plays an important role in the process of neural injury and fibrosis.