中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
8期
1601-1604
,共4页
缪苏宇%凌立君%崇梅红%王水
繆囌宇%凌立君%崇梅紅%王水
무소우%릉립군%숭매홍%왕수
乳腺癌%转移%单核甘酸多态性%Sipa1
乳腺癌%轉移%單覈甘痠多態性%Sipa1
유선암%전이%단핵감산다태성%Sipa1
Breast cancer%Metastasis%Single nucleotide polymorphism%Sipa1
目的 探讨Sipal基因rs931127和rs746429多态性与乳腺癌外周血循环肿瘤细胞的关系.方法 采用基因测序的方法,对124例原发性乳腺癌患者以及120例健康人进行Sipal基因rs931127和rs746429多态性分析.实时荧光定量聚合酶链反应(RT-qPCR)检测其中92例患者外周血循环肿瘤细胞,比较Sipal基因rs931127和rs746429多态性与外周血循环肿瘤细胞之间的关系.采用Fisher精确检验及非条件Logisitic同归法计算各组P值及相对危险度比(OR)及95％可信区间(95％ CI).结果 Sipal基因rs931127和rs746429多态性与乳腺癌患者外周血循环肿瘤细胞无明显相关(P＞0.05).Sipal基因rs931127和rs746429多态性与乳腺癌患者的年龄、肿块大小、雌激素受体(ER)、孕激素受体(PR)、C-erb-B2蛋白的表达以及乳腺癌分子分型均无明显相关(P＞0.05).但Sipal rs931127野生型GG淋巴转移率22.6％,低于杂合型GA 48.3％和突变型AA71.0％(P＜0.01),Sipal rs746429野生型CC的淋巴结转移率为31.4％,也低于杂合型CT 64.2％及突变型TT44.4％(P＜0.01).结论 Sipal基因rs931127多态性和rs746429多态性与乳腺癌患者外周血循环肿瘤细胞无明显相关,但与淋巴结转移有关.提示rs931127 GA或AA以及rs746429 CT或TT基因型可能与乳腺癌淋巴转移有关.
目的 探討Sipal基因rs931127和rs746429多態性與乳腺癌外週血循環腫瘤細胞的關繫.方法 採用基因測序的方法,對124例原髮性乳腺癌患者以及120例健康人進行Sipal基因rs931127和rs746429多態性分析.實時熒光定量聚閤酶鏈反應(RT-qPCR)檢測其中92例患者外週血循環腫瘤細胞,比較Sipal基因rs931127和rs746429多態性與外週血循環腫瘤細胞之間的關繫.採用Fisher精確檢驗及非條件Logisitic同歸法計算各組P值及相對危險度比(OR)及95％可信區間(95％ CI).結果 Sipal基因rs931127和rs746429多態性與乳腺癌患者外週血循環腫瘤細胞無明顯相關(P＞0.05).Sipal基因rs931127和rs746429多態性與乳腺癌患者的年齡、腫塊大小、雌激素受體(ER)、孕激素受體(PR)、C-erb-B2蛋白的錶達以及乳腺癌分子分型均無明顯相關(P＞0.05).但Sipal rs931127野生型GG淋巴轉移率22.6％,低于雜閤型GA 48.3％和突變型AA71.0％(P＜0.01),Sipal rs746429野生型CC的淋巴結轉移率為31.4％,也低于雜閤型CT 64.2％及突變型TT44.4％(P＜0.01).結論 Sipal基因rs931127多態性和rs746429多態性與乳腺癌患者外週血循環腫瘤細胞無明顯相關,但與淋巴結轉移有關.提示rs931127 GA或AA以及rs746429 CT或TT基因型可能與乳腺癌淋巴轉移有關.
목적 탐토Sipal기인rs931127화rs746429다태성여유선암외주혈순배종류세포적관계.방법 채용기인측서적방법,대124례원발성유선암환자이급120례건강인진행Sipal기인rs931127화rs746429다태성분석.실시형광정량취합매련반응(RT-qPCR)검측기중92례환자외주혈순배종류세포,비교Sipal기인rs931127화rs746429다태성여외주혈순배종류세포지간적관계.채용Fisher정학검험급비조건Logisitic동귀법계산각조P치급상대위험도비(OR)급95％가신구간(95％ CI).결과 Sipal기인rs931127화rs746429다태성여유선암환자외주혈순배종류세포무명현상관(P＞0.05).Sipal기인rs931127화rs746429다태성여유선암환자적년령、종괴대소、자격소수체(ER)、잉격소수체(PR)、C-erb-B2단백적표체이급유선암분자분형균무명현상관(P＞0.05).단Sipal rs931127야생형GG림파전이솔22.6％,저우잡합형GA 48.3％화돌변형AA71.0％(P＜0.01),Sipal rs746429야생형CC적림파결전이솔위31.4％,야저우잡합형CT 64.2％급돌변형TT44.4％(P＜0.01).결론 Sipal기인rs931127다태성화rs746429다태성여유선암환자외주혈순배종류세포무명현상관,단여림파결전이유관.제시rs931127 GA혹AA이급rs746429 CT혹TT기인형가능여유선암림파전이유관.
Objective To investigate the correlation between the polymorphisms (rs931127 and rs746429) in signal-induced proliferation-associated 1 ( Sipal ) gene and circulating tumor cells in patients with breast cancer.Methods The polymorphisms of Sipal were detected by using polymerase chain reaction (PCR)-DNA sequencing in 124 primary breast cancer patients and 120 healthy controls.The circulating tumor cells were detected by real-time quantitative PCR.Results The polymorphisms were not associated with the circulating tumor cells of the breast cancer patients ( P ＞ 0.05 ).rs931127 and rs746429 polymorphisms of Sipal were not significantly associated with age,estrogen receptor status,progesterone receptor status,and C-erbB2 overexpression of patients with breast cancer (P ＞ 0.05). However,patients with the rs931127 homozygous for the wild-type GG genotype were less likely to have positive lymph node involvement (22.6％) than those with heterozygous GA (48.3％) and mutant AA genotype (71.0％)(P ＜ 0.01 ).Patients with the rs746429 homozygous for the wild-type CC genotype were less likely to have positive lymph node involvement (31.4％) than patients with heterozygous CT (64.2％ ) and mutant TT genotype too (44.4％ ) ( P ＜ 0.01 ).Conclusion The variants rs746429 and the rs931127 allele were not associated with the circulating tumor cells of the breast cancer patients,but associated with lymph node involvement.rs931127 A allele and rs746429 T allele in patients with breast cancer may be associated with lymphatic metastasis.
