CAJ | 학술논문

目的研究我国多发性硬化(multiple sclerosis,Ms)患者外周血单个核细胞基因SP3(specificprotein 3)mRNA表达缺失的特点及其与临床表型的关系.方法采集56例MS患者外周血单个核细胞,提取总RNA,设计SP3特异性引物,采用逆转录PCR方法观察SP3基因mRNA表达情况,并与其他疾病组、健康对照组进行比较.结果 56例MS患者中23例SP3为阴性结果,SP3表达缺失率为41.1%;健康对照组35例有5例为阴性结果,缺失率为8.6%;其它疾病对照组27例有4例为阴性结果,缺失率为14.3%.MS组与两对照组之间的差异有统计学意义(P＜0.01).SP3表达缺失组与表达组急性期的改良残废程度量表评分差异无统计学意义,稳定期则差异有统计学意义(P＜0.05).结论通过对MS患者SP3基因mRNA表达的研究,观察到中国人MS存在该基因mRNA表达缺失.SP3与MS临床表现及其免疫学发病机制之间可能存在一定的相关性.
목적 연구아국다발성경화(multiple sclerosis,Ms)환자외주혈단개핵세포기인SP3(specificprotein 3)mRNA표체결실적특점급기여림상표형적관계.방법 채집56례MS환자외주혈단개핵세포,제취총RNA,설계SP3특이성인물,채용역전록PCR방법관찰SP3기인mRNA표체정황,병여기타질병조、건강대조조진행비교.결과 56례MS환자중23례SP3위음성결과,SP3표체결실솔위41.1%;건강대조조35례유5례위음성결과,결실솔위8.6%;기타질병대조조27례유4례위음성결과,결실솔위14.3%.MS조여량대조조지간적차이유통계학의의(P＜0.01).SP3표체결실조여표체조급성기적개량잔폐정도량표평분차이무통계학의의,은정기칙차이유통계학의의(P＜0.05).결론 통과대MS환자SP3기인mRNA표체적연구,관찰도중국인MS존재해기인mRNA표체결실.SP3여MS림상표현급기면역학발병궤제지간가능존재일정적상관성.
Objective To characterize the deficiency of the mRNA expression of specific protein 3(SP3)gene in peripheral blood mononuc|ear cells(PBMCs)from Chinese patients with multiple sclerosis(MS)and study its correlation with the disease phenotypes.Methods Fifty-six patients with definite MS were collected and total RNA was extracted from their PBMCs.Specific primers corresponding to SP3 gene were designed and the mRNA expression of SP3 gene was detected by reverse transcriptase-PCR(RT-PCR)method.The deficiency of SP3 expression was compared among MS patients,irrelavant disease group and normal controls.Results Of the 56 MS cases,23(41.1%)were SP3 -deficient.In contrast,the frequency of SP3-deficiency in normal subjects and irrelavant disease controls Was 8.6% (5/35)and 14.3%(4/27),respectively.The frequency of the SP3-expression deficiency in MS patients Was significantly higher than that in both control groups(P＜0.01).Within the MS cases,the scores of expanded disability status scale(EDSS)in the SP3-expressing subjects were significantly different from that in the SP3-deficient ones in the stable,but not in the active,phase of MS(P＜0.05).Conclusion Author's observation suggested that deficient expression of SP3 gene occurs in Chinese MS patients,and that the SP3 expression may correlate with the clinical manifestations of MS and play roles in its immunological pathogenesis.