CAJ | 학술논문

目的观察散发性乳腺癌组织中BRCA1、黄体酮受体A(progesterone receptor A,PRA)和黄体酮受体B(progesterone receptor B,PRB)的表达,并分析散发性乳腺癌BRCA1对PRA和PRB表达的影响.方法选择南方医科大学附属南方医院病理科乳腺腺病和散发性浸润性乳腺导管癌病例蜡块包埋组织各68例,应用免疫组织化学法检测散发性浸润性乳腺导管癌组织BRCA1、PRA和PRB蛋白及乳腺腺病组织BRCA1蛋白的表达,用Wilcoxon两样本检验比较浸润性导管癌和乳腺腺病BRCA1表达的高低,用x2检验分析浸润性导管癌BRCA1对PRA和PRB表达的影响.结果 (1)浸润性导管癌组织BRCA1蛋白阳性表达率为60.3%(41/68),低于乳腺腺病BRCA1蛋白阳性表达率85.3%(58/68),两者之间差异有统计学意义(P<0.01);(2)浸润性导管癌组织中,BRCA1表达阴性标本PRA的阳性率为81.5%(22/27),高于BRCA1表达阳性标本PRA的阳性率53.7%(22/47),差异有统计学意义(P<0.05),提示BRCA1可影响PRA的表达.浸润性导管癌组织中PRB的表达率在BRCA1表达阳性标本与表达阴性标本之间差异无统计学意义(P>0.05),提示BRCA1不影响PRB的表达.结论在散发性乳腺癌组织中,BRCA1表达阴性选择性地与PRA的高表达相关联,但与PRB表达率不相关联,提示BRCA1在散发性乳腺癌组织中选择性地调节PRA的表达.
목적 관찰산발성유선암조직중BRCA1、황체동수체A(progesterone receptor A,PRA)화황체동수체B(progesterone receptor B,PRB)적표체,병분석산발성유선암BRCA1대PRA화PRB표체적영향.방법 선택남방의과대학부속남방의원병이과유선선병화산발성침윤성유선도관암병례사괴포매조직각68례,응용면역조직화학법검측산발성침윤성유선도관암조직BRCA1、PRA화PRB단백급유선선병조직BRCA1단백적표체,용Wilcoxon량양본검험비교침윤성도관암화유선선병BRCA1표체적고저,용x2검험분석침윤성도관암BRCA1대PRA화PRB표체적영향.결과 (1)침윤성도관암조직BRCA1단백양성표체솔위60.3%(41/68),저우유선선병BRCA1단백양성표체솔85.3%(58/68),량자지간차이유통계학의의(P<0.01);(2)침윤성도관암조직중,BRCA1표체음성표본PRA적양성솔위81.5%(22/27),고우BRCA1표체양성표본PRA적양성솔53.7%(22/47),차이유통계학의의(P<0.05),제시BRCA1가영향PRA적표체.침윤성도관암조직중PRB적표체솔재BRCA1표체양성표본여표체음성표본지간차이무통계학의의(P>0.05),제시BRCA1불영향PRB적표체.결론 재산발성유선암조직중,BRCA1표체음성선택성지여PRA적고표체상관련,단여PRB표체솔불상관련,제시BRCA1재산발성유선암조직중선택성지조절PRA적표체.
Objective To investigate the expression level of BRCA1, progesterone receptor A (PRA) and B (PRB) in tissue of sporadic invasive ductal breast carcinoma and further statistically analyze the BRCA1 effects on the expression rates of PRA and PRB. Methods Sixty-eight cases of adenosis of breast and sporadic invasive ductal breast carcinoma were selected The corresponding paraffin-embodied tissues were collected from the archive of Department of Pathology, Nanfang Hospital. The expressions level of BRCA1, PRA and PRB were detected by immunohistochemistry. Wilcoxon two-sample test was used to analyze the differential expression of BRCA1 between adenosis and sporadic invasive ductal breast carcinoma. Chi-square test was used to analyze the effects of BRCA1 on the PRA or PRB expression rate. P value<0.05 was considered statistically significant Results (1) In invasive sporadic ductal breast carcinoma, the positive rate of BRCA1 expression of 60. 29% (41/68) was lower than the positive rate of BRCA1 expression at 85.30% (58/68) in adenosis of breast And the difference of BRCA1 expression between two groups was statistically significant (P< 0.01); (2) In invasive sporadic ductal breast carcinoma, the positive rate of PRA expression for negative BRCA1 expression samples was 81.48% (22/27) and it was higher than the positive rate of PRA expression at 53. 66% (22/47) for positive BRCA1 expression samples. And the difference of PRA expression rates between two groups was statistically significant (P<0. 05).It indicated that the expression of BRCA1 affected the expression rate of PRA; Ininvasive sporadic ductal breast carcinoma.the PRB expression rates between positive and negative BRCA1 expression samples were not statistically significant(P>0.05).It indicated that BRCA1 had no effect upon the expression rate of PRB.Conclusion In sporadic breast carcinoma.a negative expression of BRCA1 is selectively associated with a higher expression rate of PRA rather than PRB.Thus BRCA1 selectively regulates the expression of PRA in sporadic breast carcinoma.