CAJ | 학술논문

目的:探讨干扰素α对白血病Raji细胞生长的抑制作用及其作用机制.方法:以不同浓度的干扰素α作用于体外培养的Raji细胞,应用MTT法检测细胞生长抑制率,流式细胞仪检测细胞凋亡率,Hoechst 33258荧光染色法观察细胞凋亡,TRAP-PCR-ELISA法检测细胞凋亡前后的端粒酶活性.结果:5×103 U*ml-1以上的干扰素α可显著降低Raji细胞端粒酶活性,抑制细胞的生长及诱导细胞发生凋亡,并呈现出明显的量-效与时-效关系.药物(10×103 U*ml-1)作用48～60 h在Hoechst染色图片上可见核浓缩及核碎裂等典型的凋亡改变.结论:干扰素α能抑制Raji细胞的生长并诱导细胞发生调亡,降低细胞端粒酶活性可能是其重要作用机制之一;这为干扰素应用于临床治疗淋巴瘤细胞白血病提供了有力的试验依据.
목적:탐토간우소α대백혈병Raji세포생장적억제작용급기작용궤제.방법:이불동농도적간우소α작용우체외배양적Raji세포,응용MTT법검측세포생장억제솔,류식세포의검측세포조망솔,Hoechst 33258형광염색법관찰세포조망,TRAP-PCR-ELISA법검측세포조망전후적단립매활성.결과:5×103 U*ml-1이상적간우소α가현저강저Raji세포단립매활성,억제세포적생장급유도세포발생조망,병정현출명현적량-효여시-효관계.약물(10×103 U*ml-1)작용48～60 h재Hoechst염색도편상가견핵농축급핵쇄렬등전형적조망개변.결론:간우소α능억제Raji세포적생장병유도세포발생조망,강저세포단립매활성가능시기중요작용궤제지일;저위간우소응용우림상치료림파류세포백혈병제공료유력적시험의거.
AIM: To investigate the anti-proliferation effect of interferon-α(IFN-α) on leukemic Raji cells and its mechanism. METHODS: Raji cells were given different concentrations of INF-α. The inhibitory rate of the cells was measured by MTT assay, apoptotic rate was detected by flow cytometry (FCM), morphology of cell apoptosis was observed by Hoechst 33258 fluorescence staining, and the activity of telomerase was detected by PCR-ELISA before and after apoptosis occurred. RESULTS: IFN-α (over 5×103 U*ml-1) could cause apoptosis significantly, decrease the telomerase activity, and inhibit the growth of Raji cells in time-and dose-dependent manner. Marked morphological changes of cell apoptosis including chromosome condensation and nuclear fragmentation were observed very clearly by Hoechst 33258 fluorescence staining especially after the cells treated with IFN-α for 48-60 h. CONCLUSION: IFN-α has apparent anti-proliferation and apoptotic effects on Raji cells in vitro, and one of the most important mechanisms may be decrease of the telomerase activity of Raji cells. These results will provide strong laboratory evidence of IFN-α in the clinical treatment of lymphoma cell leukemia.