中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2005年
13期
172-174
,共3页
邓学军%孙圣刚%曹学兵%李红戈%梁直厚
鄧學軍%孫聖剛%曹學兵%李紅戈%樑直厚
산학군%손골강%조학병%리홍과%량직후
丙酸类%神经母细胞瘤%1-甲基-4苯基吡啶离子
丙痠類%神經母細胞瘤%1-甲基-4苯基吡啶離子
병산류%신경모세포류%1-갑기-4분기필정리자
背景:3-硝基丙酸可以抑制氧化磷酸化过程,损害细胞的能量代谢从而引起细胞的损伤.但是小剂量的3-硝基丙酸却可以通过轻度抑制细胞的氧化磷酸化过程,激发细胞内源性保护因子保护神经元,增加神经元对缺血缺氧的耐受性,但它对多巴胺能神经元是否也有类似的作用尚不十分清楚.目的:探讨3-硝基丙酸预处理能否增强多巴胺能神经元对1-甲基-4苯基吡啶离子毒性的耐受.设计:以能分泌多巴胺的神经母细胞瘤SH-SY5Y细胞为研究对象,进行随机对照的探索性研究.单位:一所大学附属医院的神经科.材料:实验于2003-03/11在同济医学院病理生理实验室完成.SH-SY5Y细胞购于北京协和医科大学细胞典藏中心.干预:细胞随机分为6组,将1-甲基-4苯基吡啶离子加入到培养的多巴胺能神经元SH-SY5Y细胞中制作帕金森病的细胞模型,在加入1-甲基-4苯基吡啶离子(0.25 mmol/L)前,分别单次或多次加入3-硝基丙酸(0.2 mmol/L)形成预处理,应用四氮唑盐检测细胞生存率,[3H]DA摄取率测定多巴胺能细胞突触前功能.主要观察指标:主要结局:细胞生存率.次要结局:[3H]DA摄取率.结果:1-甲基-4苯基吡啶离子组细胞生存率(54.3%)较空白对照组明显降低(P＜0.01),3-硝基丙酸预处理后,细胞生存率明显增高,分别为71.8%(单次),85.2%(多次),单次预处理组与多次预处理组相比也有显著性差异(P＜0.05).[3H]DA摄取率结果与细胞生存率结果相似.[3H]DA摄取率分别为65.8%(单次),80.3%(多次),较1-甲基-4苯基吡啶离子组(50.1%)明显提高,且多次预处理较单次预处理效果更好.单加3-硝基丙酸对细胞无影响.结论:3-硝基丙酸预处理可明显增强多巴胺能神经元对1-甲基-4苯基吡啶离子毒性作用的耐受性,对多巴胺能神经元有明显的保护作用,多次预处理的保护作用更加显著.
揹景:3-硝基丙痠可以抑製氧化燐痠化過程,損害細胞的能量代謝從而引起細胞的損傷.但是小劑量的3-硝基丙痠卻可以通過輕度抑製細胞的氧化燐痠化過程,激髮細胞內源性保護因子保護神經元,增加神經元對缺血缺氧的耐受性,但它對多巴胺能神經元是否也有類似的作用尚不十分清楚.目的:探討3-硝基丙痠預處理能否增彊多巴胺能神經元對1-甲基-4苯基吡啶離子毒性的耐受.設計:以能分泌多巴胺的神經母細胞瘤SH-SY5Y細胞為研究對象,進行隨機對照的探索性研究.單位:一所大學附屬醫院的神經科.材料:實驗于2003-03/11在同濟醫學院病理生理實驗室完成.SH-SY5Y細胞購于北京協和醫科大學細胞典藏中心.榦預:細胞隨機分為6組,將1-甲基-4苯基吡啶離子加入到培養的多巴胺能神經元SH-SY5Y細胞中製作帕金森病的細胞模型,在加入1-甲基-4苯基吡啶離子(0.25 mmol/L)前,分彆單次或多次加入3-硝基丙痠(0.2 mmol/L)形成預處理,應用四氮唑鹽檢測細胞生存率,[3H]DA攝取率測定多巴胺能細胞突觸前功能.主要觀察指標:主要結跼:細胞生存率.次要結跼:[3H]DA攝取率.結果:1-甲基-4苯基吡啶離子組細胞生存率(54.3%)較空白對照組明顯降低(P＜0.01),3-硝基丙痠預處理後,細胞生存率明顯增高,分彆為71.8%(單次),85.2%(多次),單次預處理組與多次預處理組相比也有顯著性差異(P＜0.05).[3H]DA攝取率結果與細胞生存率結果相似.[3H]DA攝取率分彆為65.8%(單次),80.3%(多次),較1-甲基-4苯基吡啶離子組(50.1%)明顯提高,且多次預處理較單次預處理效果更好.單加3-硝基丙痠對細胞無影響.結論:3-硝基丙痠預處理可明顯增彊多巴胺能神經元對1-甲基-4苯基吡啶離子毒性作用的耐受性,對多巴胺能神經元有明顯的保護作用,多次預處理的保護作用更加顯著.
배경:3-초기병산가이억제양화린산화과정,손해세포적능량대사종이인기세포적손상.단시소제량적3-초기병산각가이통과경도억제세포적양화린산화과정,격발세포내원성보호인자보호신경원,증가신경원대결혈결양적내수성,단타대다파알능신경원시부야유유사적작용상불십분청초.목적:탐토3-초기병산예처리능부증강다파알능신경원대1-갑기-4분기필정리자독성적내수.설계:이능분비다파알적신경모세포류SH-SY5Y세포위연구대상,진행수궤대조적탐색성연구.단위:일소대학부속의원적신경과.재료:실험우2003-03/11재동제의학원병리생리실험실완성.SH-SY5Y세포구우북경협화의과대학세포전장중심.간예:세포수궤분위6조,장1-갑기-4분기필정리자가입도배양적다파알능신경원SH-SY5Y세포중제작파금삼병적세포모형,재가입1-갑기-4분기필정리자(0.25 mmol/L)전,분별단차혹다차가입3-초기병산(0.2 mmol/L)형성예처리,응용사담서염검측세포생존솔,[3H]DA섭취솔측정다파알능세포돌촉전공능.주요관찰지표:주요결국:세포생존솔.차요결국:[3H]DA섭취솔.결과:1-갑기-4분기필정리자조세포생존솔(54.3%)교공백대조조명현강저(P＜0.01),3-초기병산예처리후,세포생존솔명현증고,분별위71.8%(단차),85.2%(다차),단차예처리조여다차예처리조상비야유현저성차이(P＜0.05).[3H]DA섭취솔결과여세포생존솔결과상사.[3H]DA섭취솔분별위65.8%(단차),80.3%(다차),교1-갑기-4분기필정리자조(50.1%)명현제고,차다차예처리교단차예처리효과경호.단가3-초기병산대세포무영향.결론:3-초기병산예처리가명현증강다파알능신경원대1-갑기-4분기필정리자독성작용적내수성,대다파알능신경원유명현적보호작용,다차예처리적보호작용경가현저.
BACKGROUND: 3-nitropropionic acid(3-NP) can inhibit the process of oxidative phosphorylation and injure the energy metabolism of the cell and thereby induce cell injury. However, small dose of 3-NP can excite intrinsic cellular protective factor to protect neurons and increase the tolerance of neurons to ischemic hypoxia through mild inhibiting the process of oxidative phosphorylation. It is unclear whether it also has the similar effect on dopaminergic neurons.OBJECTIVE: To investigate whether 3-NP preconditioning could enhance the tolerance of dopaminergic neurons to MPP+(1-methyl-4-phenylpyridine)toxicity.DESIGN: A randomized controlled exploring research based on neuroblastoma SH-SYSY cell that could secrete dopamine.SETTING: Department of neurology of a university hospital.MATERIALS: The study was conducted in the Laboratory of Pathophysiology of Tongji Medical College between March 2003 and November 2003. SH-SYSY cell was obtained from the Cell Center of Peking Union Medical University.INTERVENTIONS: Cells were randomly divided into 6 groups. MPP+ was added into the cultured dopaminergic neuron SH-SY5Y cells for the establishment of the cell model for Parkinson disease. Before the admission of MPP+ (0.25 mmol/L), 3-NP(0. 2 mmol/L) was added once or repetitive times to form preconditioning. Microculture tetrozolium(MTT) was used to detect cell survival rate, and[3H] DA uptake rate was used to test the anterior synaptic function of dopaminergic neurons.MAIN OUTCOME MEASURES: Major consequence: Cell survival rate;Minor consequence: [3H] DA uptake rate.RESULTS: Cell survival rate of MPP+ group was 54.3%, which was significantly lower than that of blank control group( P ＜ 0.01) . After 3-NP preconditioning, cell survival rate significantly elevated, which was 71.8%(single) or 85.2% (repetitive) . There was significant difference between single preconditioning and repetitive preconditioning( P ＜ 0.05 ). The results of[3H] DA uptake rate were similar to that of cell survival rate. [3H] DA uptake rate was 65.8% (single) or 80. 3% (repetitive), which was significantly higher than 50. 1% of MPP+ group. And moreover, repetitive preconditioning had more favorable effect than single preconditioning. Simple admission of 3-NP had no impact on cells.CONCLUSION: 3-NP preconditioning can significantly enhance the tolerance of dopaminergic neuron to MPP+ toxicity, which has significant protective effects on dopaminergic neuron. Repetitive preconditioning have more significant protective effects.
