CAJ | 학술논문

背景:啤酒对血清酶活性的直接影响尚未见报道.目的:观察体内外实验中受试者饮用啤酒后各种血清酶活性的变化情况.设计:观察对比实验.单位:泰山医学院基础医学研究所.对象:实验于2005-03/04在泰山医学院基础医学研究所完成.选择泰山医学院学生17名,年龄19～35岁,包括本科生和研究生,实验前均签署同意书.方法:①体内实验:受试者统一正常饮食后3 h采静脉血3 mL,作为对照.然后立即口服啤酒4 mL/kg,分别于15,30,45,60,90,120,180 min后各采血3 mL,测定血液中丙氨酸氨基转移酶、天冬氨酸氨基转移酶、γ-谷氨酰转肽酶、碱性磷酸酶、肌酸激酶、乳酸脱氢酶、淀粉酶、脂肪酶活性的变化.②体外实验:选取17份新鲜受试者血清,分别加入两个试管中,每管0.5 mL.对照管加入20 μL生理盐水;测定管中加入20 μL啤酒溶液,观察啤酒对以上各种酶活性的直接影响.主要观察指标:体内外实验中血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶、γ-谷氨酰转肽酶、碱性磷酸酶、肌酸激酶、乳酸脱氢酶、淀粉酶及脂肪酶的活性.结果:纳入17名学生,全部进入结果分析,无脱落.①体内实验:啤酒可显著降低血清天冬氨酸氨基转移酶活性(418.08±58.68,383.41±63.01)nkat/L,显著升高血清碱性磷酸酶活性(3 678.57 ±436.25,3 962.96±400.91)nkat/L(x2=19.00～20.00,P＜0.01),其余酶活性均有不同程度的升高.②体外实验:啤酒在体外对各种酶活性均有一定程度的抑制作用.结论:啤酒在体内外对酶活性均有一定影响,从而影响机体代谢,过量饮用会影响健康.在常规血清酶学检测中,应避免患者饮用啤酒所造成的干扰,以确保实验结果的准确可靠.
배경:비주대혈청매활성적직접영향상미견보도.목적:관찰체내외실험중수시자음용비주후각충혈청매활성적변화정황.설계:관찰대비실험.단위:태산의학원기출의학연구소.대상:실험우2005-03/04재태산의학원기출의학연구소완성.선택태산의학원학생17명,년령19～35세,포괄본과생화연구생,실험전균첨서동의서.방법:①체내실험:수시자통일정상음식후3 h채정맥혈3 mL,작위대조.연후립즉구복비주4 mL/kg,분별우15,30,45,60,90,120,180 min후각채혈3 mL,측정혈액중병안산안기전이매、천동안산안기전이매、γ-곡안선전태매、감성린산매、기산격매、유산탈경매、정분매、지방매활성적변화.②체외실험:선취17빈신선수시자혈청,분별가입량개시관중,매관0.5 mL.대조관가입20 μL생리염수;측정관중가입20 μL비주용액,관찰비주대이상각충매활성적직접영향.주요관찰지표:체내외실험중혈청병안산안기전이매、천동안산안기전이매、γ-곡안선전태매、감성린산매、기산격매、유산탈경매、정분매급지방매적활성.결과:납입17명학생,전부진입결과분석,무탈락.①체내실험:비주가현저강저혈청천동안산안기전이매활성(418.08±58.68,383.41±63.01)nkat/L,현저승고혈청감성린산매활성(3 678.57 ±436.25,3 962.96±400.91)nkat/L(x2=19.00～20.00,P＜0.01),기여매활성균유불동정도적승고.②체외실험:비주재체외대각충매활성균유일정정도적억제작용.결론:비주재체내외대매활성균유일정영향,종이영향궤체대사,과량음용회영향건강.재상규혈청매학검측중,응피면환자음용비주소조성적간우,이학보실험결과적준학가고.
BACKGROUND: There have been no reports available about the direct effect of beer on serum enzyme activity.OBJECTIVE: To observe the changes of the activity of various serum enzymes of the subjects in the in vivo and in vitro experiments after drinking bear.DESIGN: An observational controlled experiment.SETTING: The Institute of Basic Medicine of Taishan Medical College.PARTICIPANTS: The experiment was performed at the Institute of Basic Medicine of Taishan Medical College between March 2005 and April 2005.We selected 17 college students, aged 19 to 35 years, from Taishan Medical College, including undergraduate students and graduate students. In formed consents were obtained from the subjects before the experiment was conducted.METHODS: ① In vivo experiment: 3 mL of venous blood was collected from the subjects 3 hours after the ordinary diet as the control. Then, the subjects drank beer at an amount of 4 mL/kg according to their body mass immediately. 3 mL of blood was collected respectively 15, 30, 45, 60, 90,120, 80 minutes later to measure the changes of the activity of alanine aminotransferase, aspartate aminotransferase, γ-glutamyl transpeptidase, al kaline phosphatase, creatine kinase , lactate dehydrogenase, diastase and lipase. ② In vitro experiment: 17 fresh serum samples were added into two test tubes separately with 0.5 mL of serum in each tube. 20 μL of normal saline was added to the tube of the control and 20 μL of beer was added into the test tube. The direct effect of beer on the activity of various enzymes was observed.MAIN OUTCOME MEASURES: The changes of the activity of alanine aminotransferase, aspartate aminotransferase, γ-glutamyl transpeptidase, al kaline phosphatase, creatine kinase , lactate dehydrogenase, diastase and lipase of the serum on the in vivo and in vitro experiment .RESULTS: Totally 17 students were involved and all the students entered the stage of result analysis with no loss in the midway. ① In vivo experiment: Beer significantly decreased the activity of serum aspartate aminotransferase (418.08 ±58.68,383.41 ±63.01)nkat/L, significantly in creased the activity of serum alkaline phosphatase, (367 8.57±436.25,396 2.96±400.91)nkat/L (x2=19.00-20.00,P ＜ 0.01). The activity of other enzymes was all increased at different degrees. ② In vitro experiment: Beer inhibited the activity of various enzymes in vitro to a certain degree.CONCLUSION: Beer has some effect on enzyme activity in vivo and in vitro, thus affecting the body metabolism. Over drinking beer can affect health. In the routine detection of serum enzymes, attention should be given to avoid the interference caused by over drinking beer to make sure the experimental results are precise and reliable.