化学通报（印刷版）
化學通報（印刷版）
화학통보（인쇄판）
CHEMISTRY
2009年
11期
1008-1012
,共5页
脂肽%地衣素%分离%结构%地衣芽胞杆菌
脂肽%地衣素%分離%結構%地衣芽胞桿菌
지태%지의소%분리%결구%지의아포간균
Lipopeptide%Lichenysin%Separation%Stractrural characterization%Bacillus licheniformis
经过酸沉淀分离、有机溶剂萃取和二步柱分离,从地衣芽胞杆菌(Bacillus licheniformis)HSN 221菌株的培养液中分离纯化得到了一种地衣素类似物.经ESI-MS测定,该物质的分子量为1062Da;经对酸解后甲醇酯化的脂肪酸的GC/MS分析可知,该物质含有3-羟基正十六烷酸残基;Q-TOF MS/MS的结果显示该物质具有GlnLeuLeuVMAspMeLeuIle的肽链,因此,该物质为甲酯化的n-C16-地衣素.
經過痠沉澱分離、有機溶劑萃取和二步柱分離,從地衣芽胞桿菌(Bacillus licheniformis)HSN 221菌株的培養液中分離純化得到瞭一種地衣素類似物.經ESI-MS測定,該物質的分子量為1062Da;經對痠解後甲醇酯化的脂肪痠的GC/MS分析可知,該物質含有3-羥基正十六烷痠殘基;Q-TOF MS/MS的結果顯示該物質具有GlnLeuLeuVMAspMeLeuIle的肽鏈,因此,該物質為甲酯化的n-C16-地衣素.
경과산침정분리、유궤용제췌취화이보주분리,종지의아포간균(Bacillus licheniformis)HSN 221균주적배양액중분리순화득도료일충지의소유사물.경ESI-MS측정,해물질적분자량위1062Da;경대산해후갑순지화적지방산적GC/MS분석가지,해물질함유3-간기정십륙완산잔기;Q-TOF MS/MS적결과현시해물질구유GlnLeuLeuVMAspMeLeuIle적태련,인차,해물질위갑지화적n-C16-지의소.
The lipopepfide produced by Bacillus licheniformis HSN 221 was separated from the cell-free broth by the methods of acid precipitating, solvent extracting and purified by two-step column separation. Its molecular structure was determined by ESI-MS, GC/MS and Q-TOF MS/MS. The results showed that the lipopeptide with MW of 1062Da contains 3-hydroxylhexadecoic acid and peptide chain of GlnLeuLeuValAspMeLeuIle, which indicates that it is an esterified n-C16-lichenysin with methanol.
