CAJ | 학술논문

目的:探讨白藜芦醇对兔动脉粥样硬化斑块内细胞凋亡及Fas、FasL、Caspase-3基因转录的影响.方法:成熟健康雄性新西兰白兔70只,适应性喂养10 d后随机分为5组:A组继续喂普通饲料;B组喂高脂饲料;C、D、E组喂高脂饲料的同时分别给予白藜芦醇4 mg·kg~(-1)·d~(-1)、8 mg·kg~(-1)·d~(-1)、16 mg·kg~(-1)·d~(-1)进行干预.末端脱氧核苷酸转移酶介导的脱氧三磷酸尿苷缺口末端标记法(TUNEL)检测细胞凋亡;逆转录聚合酶链式反应法(RT-PCR)检测Fas、FasL、Caspase-3mRNA转录水平.结果:高脂喂养12周后,成功建立动脉粥样硬化模型;病理对照组斑块内有大量的凋亡细胞;与病理对照组比较,正常对照组血管内膜偶见少量的凋亡细胞(P<0.01),低至高剂量白藜芦醇干预组细胞凋亡依次降低(P<0.05),其Fas mRNA、FasL mRNA转录水平也依次降低,白藜芦醇高剂量组Fas mRNA转录水平与正常对照组比较差异无统计学意义(P>0.05).结论:白藜芦醇可抑制动脉粥样斑块内细胞凋亡,从而稳定斑块、抑制动脉粥样病变进展,这一作用可能通过抑制Fas/FasL凋亡途径,降低Caspase-3基因转录来实现,并具有一定的量效关系.
목적:탐토백려호순대토동맥죽양경화반괴내세포조망급Fas、FasL、Caspase-3기인전록적영향.방법:성숙건강웅성신서란백토70지,괄응성위양10 d후수궤분위5조:A조계속위보통사료;B조위고지사료;C、D、E조위고지사료적동시분별급여백려호순4 mg·kg~(-1)·d~(-1)、8 mg·kg~(-1)·d~(-1)、16 mg·kg~(-1)·d~(-1)진행간예.말단탈양핵감산전이매개도적탈양삼린산뇨감결구말단표기법(TUNEL)검측세포조망;역전록취합매련식반응법(RT-PCR)검측Fas、FasL、Caspase-3mRNA전록수평.결과:고지위양12주후,성공건립동맥죽양경화모형;병리대조조반괴내유대량적조망세포;여병리대조조비교,정상대조조혈관내막우견소량적조망세포(P<0.01),저지고제량백려호순간예조세포조망의차강저(P<0.05),기Fas mRNA、FasL mRNA전록수평야의차강저,백려호순고제량조Fas mRNA전록수평여정상대조조비교차이무통계학의의(P>0.05).결론:백려호순가억제동맥죽양반괴내세포조망,종이은정반괴、억제동맥죽양병변진전,저일작용가능통과억제Fas/FasL조망도경,강저Caspase-3기인전록래실현,병구유일정적량효관계.
Objective:To investigate the effect of resveratrol on FasmRNA, FasLmRNA and Caspase-3mRNA transcription in rabbits with atherosclerosis.Method:Seventy male New Zealand white rabbits were randomly divided into 5 groups:A normal control group,B pathological control group, C pathological control group treated with low dosage resveratrol(4mg·kg~(-1)·d~(-1)),D pathological group teated with mid dosage resveratrol(8 mg·kg~(-1)·d~(-1))and E pathological group treated with high dosage resveratrol(16mg·kg~(-1)·d~(-1)).At the end of the 12th week, apoptotic cells were detected by terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling. The mRNA expression of Fas, FasL and caspase-3 was examined by RT-PCR. The analysis of ANOVA by SSPS software package was used to analyses the data.Result:The atherosclerosis model with rabbits had been successfully established with high fat diet. Apoptotic index in group B was significantly increased when compared to group A (P<0.01). Resveratrol inhibited apoptosis of cells in atherosclerotic plaque. There were large quantity of FasmRNA and FasLmRNA in AS plaques in group B, but small quantity of them in normal control group(P<0.01). The expression of FasmRNA and FasLmRNA in group D and group E was significantly decreased than group B (dose dependent) (P<0.01). Compared with group A, the expression of FasmRNA did not increase significantly (P>0.05).Conclusion:Resveratrol can inhibit apoptosis of cells in atherosclerotic plaque of atherosclerosis rabbits, have plaque stabilization effects and inhibit the progress of atherosclerosis. It could inhibit the Fas/FasL apoptotic pathway, and depress the expression of caspase-3 mRNA. This effect has dose-effect relationship.