中国急救医学
中國急救醫學
중국급구의학
CHINESE JOURNAL OF CRITICAL CARE MEDICINE
2010年
4期
326-329,彩插1
,共5页
异甘草酸镁%百草枯%肺纤维化%治疗作用
異甘草痠鎂%百草枯%肺纖維化%治療作用
이감초산미%백초고%폐섬유화%치료작용
Magnesium Isoglycyrrhizinate injection%Paraquat%Pulmonary fibrosis%Therapeutic effect
目的 探讨异甘草酸镁(MgIG)对大鼠百草枯(paraquat,PQ)中毒所致肺纤维化的防治作用及可能的机制.方法 健康的SD雄性大鼠30只,随机分成5组:正常对照组、染毒对照组、MgIG低剂量(15 mg/kg)组、MgIG中剂量(30 mg/kg)组和MgIG高剂量(45 mg/kg)组;用20% PQ溶液按15 mg/kg行腹腔注射制作大鼠PQ中毒肺损伤模型,各治疗组于染毒后24 h按相应剂量MgIG行腹腔注射,正常对照组及染毒对照组注射生理盐水(按MgIG中剂量组的相应容量计算),直至死亡或被处死.于染毒后第14天全部处死,取肺组织行HE染色及Masson染色行病理学观察,并测定肺组织内羟脯氨酸(HYP)含量,同时取血清测定细胞间黏附因子-1(ICAM-1)及基质金属蛋白酶-9(MMP-9)含量.结果 所有大鼠均无自然死亡;光镜观察到染毒对照组静脉瘀血、淋巴增生、纤维母细胞增多,肺泡上皮增生明显,间质增厚.与染毒对照组相比,MgIG中剂量组的肺组织瘀血、水肿明显减轻,肺纤维化病理表现明显较轻,且肺内HYP明显下降(P均<0.01);血清中ICAM-1及MMP-9含量也明显降低(P<0.05或P<0.01).结论 MgIG中剂量对百草枯中毒大鼠肺组织纤维化损伤有一定的防治作用.
目的 探討異甘草痠鎂(MgIG)對大鼠百草枯(paraquat,PQ)中毒所緻肺纖維化的防治作用及可能的機製.方法 健康的SD雄性大鼠30隻,隨機分成5組:正常對照組、染毒對照組、MgIG低劑量(15 mg/kg)組、MgIG中劑量(30 mg/kg)組和MgIG高劑量(45 mg/kg)組;用20% PQ溶液按15 mg/kg行腹腔註射製作大鼠PQ中毒肺損傷模型,各治療組于染毒後24 h按相應劑量MgIG行腹腔註射,正常對照組及染毒對照組註射生理鹽水(按MgIG中劑量組的相應容量計算),直至死亡或被處死.于染毒後第14天全部處死,取肺組織行HE染色及Masson染色行病理學觀察,併測定肺組織內羥脯氨痠(HYP)含量,同時取血清測定細胞間黏附因子-1(ICAM-1)及基質金屬蛋白酶-9(MMP-9)含量.結果 所有大鼠均無自然死亡;光鏡觀察到染毒對照組靜脈瘀血、淋巴增生、纖維母細胞增多,肺泡上皮增生明顯,間質增厚.與染毒對照組相比,MgIG中劑量組的肺組織瘀血、水腫明顯減輕,肺纖維化病理錶現明顯較輕,且肺內HYP明顯下降(P均<0.01);血清中ICAM-1及MMP-9含量也明顯降低(P<0.05或P<0.01).結論 MgIG中劑量對百草枯中毒大鼠肺組織纖維化損傷有一定的防治作用.
목적 탐토이감초산미(MgIG)대대서백초고(paraquat,PQ)중독소치폐섬유화적방치작용급가능적궤제.방법 건강적SD웅성대서30지,수궤분성5조:정상대조조、염독대조조、MgIG저제량(15 mg/kg)조、MgIG중제량(30 mg/kg)조화MgIG고제량(45 mg/kg)조;용20% PQ용액안15 mg/kg행복강주사제작대서PQ중독폐손상모형,각치료조우염독후24 h안상응제량MgIG행복강주사,정상대조조급염독대조조주사생리염수(안MgIG중제량조적상응용량계산),직지사망혹피처사.우염독후제14천전부처사,취폐조직행HE염색급Masson염색행병이학관찰,병측정폐조직내간포안산(HYP)함량,동시취혈청측정세포간점부인자-1(ICAM-1)급기질금속단백매-9(MMP-9)함량.결과 소유대서균무자연사망;광경관찰도염독대조조정맥어혈、림파증생、섬유모세포증다,폐포상피증생명현,간질증후.여염독대조조상비,MgIG중제량조적폐조직어혈、수종명현감경,폐섬유화병리표현명현교경,차폐내HYP명현하강(P균<0.01);혈청중ICAM-1급MMP-9함량야명현강저(P<0.05혹P<0.01).결론 MgIG중제량대백초고중독대서폐조직섬유화손상유일정적방치작용.
Objective To explore the therapeutic effect and the possible mechanism of Magnesium Isoglycyrrhizinate (MgIG) on pulmonary fibrosis induced by paraquat in rats. Methods Thirty healthy male SD rats were randomly divided into five groups: normal control group, toxicosis control group, MgIG low-dose group (15 mg/kg), MgIG medium-dose group(30 mg/kg) and MgIG high-dose group(45 mg/kg). 20% paraquat was injected peritoneally at the dose of 15 mg/kg to induce pulmonary injury in rats. MgIG was injected peritoneally in every therapeutic groups after 24 hours of intoxication,and the normal control group received saline until death or being sacrificed. Rats were sacrificed after 14 days of intoxication. HE stain and Masson stain were performed and hydroxyproline(HYP) were measured for lung specimens, and serum levels of intercellular adhesion molecule-1(ICAM-1) and matrix metalloproteinase-9(MMP-9) were measured. Results No animal died during the experiment. Venous congestion or hemorrhage,lymph hyperplasia, fibroblast hyperplasia, alveolar epithelium hyperplasia and stroma thickening in part of the lung were observed in toxicosis control group under light microscope. Compared with toxicosis control group, lung congestion and edema of MgIG medium-dose group were obviously alleviated in all therapeutcal groups. The level of HYP in lung tissues decreased significantly (P<0.01), and the levels of ICAM-1 and MMP-9 in plasma decreased as well (P<0.05 and P<0.01). Conclusion Pulmonary fibrosis induced by paraquat in rats could be alleviated by administration of medium-dose MgIG .
