CAJ | 학술논문

目的:探讨胆碱与叶酸不足对人成淋巴细胞株细胞凋亡(apoptosis,APO)和坏死(necrosis, NEC)的影响. 方法:采用含0～21.5μmol/L氯化胆碱与30～240 nmol/L叶酸的24种浓度组合的修饰RPMI-1640培养基,培养人成淋巴细胞株9 d后,利用胞质阻断微核细胞分析(cytokinesis-block micronucleus cytome assay,CBMN Cyt)评价上述处理与细胞凋亡及细胞坏死的关系. 结果:APO及NEC频率随叶酸和氯化胆碱浓度升高逐渐降低,当叶酸在120 nmol/L及以上浓度、氯化胆碱在6 μmol/L及以上浓度时,APO及NEC频率均显著降低并维持在基本恒定水平,与对照组比较,差异均具有统计学意义(P<0.01或P<0.05);氯化胆碱和叶酸浓度对APO及NEC频率变化的贡献率均极显著(P<0.01),但未发现二者对细胞死亡的交互作用. 结论:胆碱与叶酸不足可诱导人成淋巴细胞株细胞死亡,6 μmol/L氯化胆碱及120 nmol/L叶酸是维持体外培养的人成淋巴细胞株APO及NEC频率在最低水平的最适组合浓度.
목적:탐토담감여협산불족대인성림파세포주세포조망(apoptosis,APO)화배사(necrosis, NEC)적영향. 방법:채용함0～21.5μmol/L록화담감여30～240 nmol/L협산적24충농도조합적수식RPMI-1640배양기,배양인성림파세포주9 d후,이용포질조단미핵세포분석(cytokinesis-block micronucleus cytome assay,CBMN Cyt)평개상술처리여세포조망급세포배사적관계. 결과:APO급NEC빈솔수협산화록화담감농도승고축점강저,당협산재120 nmol/L급이상농도、록화담감재6 μmol/L급이상농도시,APO급NEC빈솔균현저강저병유지재기본항정수평,여대조조비교,차이균구유통계학의의(P<0.01혹P<0.05);록화담감화협산농도대APO급NEC빈솔변화적공헌솔균겁현저(P<0.01),단미발현이자대세포사망적교호작용. 결론:담감여협산불족가유도인성림파세포주세포사망,6 μmol/L록화담감급120 nmol/L협산시유지체외배양적인성림파세포주APO급NEC빈솔재최저수평적최괄조합농도.
OBJECTIVE: To explore the effects of choline chloride (CC) and folic acid (FA) deficiency on apoptosis(APO) and necrosis (NEC) in human lymphoblast cell line. METHODS: Lymphoblasts were cultured in the special RPMI-1640 medium which was modified with 24 different combination of CC(0 ～ 21.5 μmol/L) and FA (30 ～ 240 nmol/L) . Cytokinesis-block micronucleus cytome assay (CBMN Cyt) was used to evaluate frequencies of APO and NEC after 9 d of culture. RESULTS: The frequencies of APO and NEC were gradually reduced as the concentrations of FA and CC increased. These 2 biomarkers were significantly decreased at and over 120 nmol/L FA and 6 μmol/L CC(P<0.01 or 0.05). Each CC and FA concentration had significant impact on variation of APO and NEC(P<0.01) but no interaction was found. CONCLUSION: Choline and folic acid deficiencies could induce cell death in human lymphoblast cell line, and 6 μmol/L CC and 120 nmol/L FA was the optimal combination for keeping the lowest level of APO and NEC in human lymphoblast cell line.