CAJ | 학술논문

采用活性追踪分离的方法,从盾叶薯蓣内生真菌菌株Dzf13中分离到两个具抗细菌活性的化合物1和2,经理化和波谱分析鉴定为麦角甾-5,7,22-三烯-3β-醇(1)和5α,8α-过氧麦角甾-6,22-二烯-3β-醇(2).结果表明,化合物1和2对根癌土壤杆菌、黄瓜角斑病菌和番茄疮痂病菌等三种植物病原细菌的生长均表现出一定的抑制活性,尤其对黄瓜角斑病菌的抑制活性较强.结果还表明5α,8α-过氧麦角甾-6,22-二烯-3β-醇(2)的抗细菌活性要比麦角甾-5,7,22-三烯-3β-醇(1)强.
채용활성추종분리적방법,종순협서여내생진균균주Dzf13중분리도량개구항세균활성적화합물1화2,경이화화파보분석감정위맥각치-5,7,22-삼희-3β-순(1)화5α,8α-과양맥각치-6,22-이희-3β-순(2).결과표명,화합물1화2대근암토양간균、황과각반병균화번가창가병균등삼충식물병원세균적생장균표현출일정적억제활성,우기대황과각반병균적억제활성교강.결과환표명5α,8α-과양맥각치-6,22-이희-3β-순(2)적항세균활성요비맥각치-5,7,22-삼희-3β-순(1)강.
Two antibacterial compounds,ergosta-5,7,22-trien-3β-ol and 5α,8α-epidioxyergosta-6,22-dien-3β-ol,were isolated and identified by bioassay-guided fractionation from the extract of the endophytic fungal isolate Dzf13 from the rhizomes of Dioscorea zingiberensis,a dioscoreaceous medicinal plant.Both two compounds were screened and have inhibitory activity on plant pathogenic bacteria such as Agrobacterium tumefaciens,Pseudomonas lachrymans and Xanthomonas vesicatoria by using the microplate-MTT colorimetric assay.Of three bacterial pathogens.P.lacrymans waS most effectively inhibited.In addition,5α,8α-epidloxyergosta-6,22-dien-3β-ol was found to be more bioactive than ergosta-5,7,22-trien-3β-ol.