中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2012年
8期
687-689
,共3页
孙国柱%杨连琦%陈盼虎%赵宗茂%韩仰军
孫國柱%楊連琦%陳盼虎%趙宗茂%韓仰軍
손국주%양련기%진반호%조종무%한앙군
脑损伤%液压冲击%神经功能评分%组织学
腦損傷%液壓遲擊%神經功能評分%組織學
뇌손상%액압충격%신경공능평분%조직학
Brain injury%Fluid percussion%Nervous function score%Histology
目的 观察不同冲击压力液压冲击脑损伤大鼠神经功能评分差异性,探讨与病理学变化的关系及其临床意义.方法 采用0.1,0.2,0.3 NPa三种不同打击压力,制作不同冲击压力大鼠液压冲击脑损伤模型,观察生命体征变化、比较死亡率,观察造模后1h、6h、12h、24h、3d、7d时神经功能评分和脑水含量,比较其组织学演变趋势.结果 应用0.1MPa冲击,动物出现一过性呼吸减弱,死亡率2.08％,神经功能评分最低降至(7.17±0.75)分,但恢复较快,脑含水量最高达(81.12±0.03)％,脑损伤仅累及冲击伤处脑皮层的浅层;应用0.2MPa冲击,动物呼吸暂停时间(10.88±2.69)s,死亡率4.17％,神经功能评分最低降至(4.83±0.75)分,脑水含量最高达(82.74±1.11)％,脑损伤深达海马结构;应用0.3MPa冲击,大鼠呼吸暂停时间(20.60±3.02)s,死亡率16.67％,神经功能评分最低降至(2.67±0.52)分,而且恢复较慢,脑水含量最高达(83.89±0.04)％,脑损伤深达海马下结构、脑干.结论 轻、中、重三种不同冲击压力液压脑损伤大鼠神经功能评分依次降低,与其病理学的严重程度呈正相关.
目的 觀察不同遲擊壓力液壓遲擊腦損傷大鼠神經功能評分差異性,探討與病理學變化的關繫及其臨床意義.方法 採用0.1,0.2,0.3 NPa三種不同打擊壓力,製作不同遲擊壓力大鼠液壓遲擊腦損傷模型,觀察生命體徵變化、比較死亡率,觀察造模後1h、6h、12h、24h、3d、7d時神經功能評分和腦水含量,比較其組織學縯變趨勢.結果 應用0.1MPa遲擊,動物齣現一過性呼吸減弱,死亡率2.08％,神經功能評分最低降至(7.17±0.75)分,但恢複較快,腦含水量最高達(81.12±0.03)％,腦損傷僅纍及遲擊傷處腦皮層的淺層;應用0.2MPa遲擊,動物呼吸暫停時間(10.88±2.69)s,死亡率4.17％,神經功能評分最低降至(4.83±0.75)分,腦水含量最高達(82.74±1.11)％,腦損傷深達海馬結構;應用0.3MPa遲擊,大鼠呼吸暫停時間(20.60±3.02)s,死亡率16.67％,神經功能評分最低降至(2.67±0.52)分,而且恢複較慢,腦水含量最高達(83.89±0.04)％,腦損傷深達海馬下結構、腦榦.結論 輕、中、重三種不同遲擊壓力液壓腦損傷大鼠神經功能評分依次降低,與其病理學的嚴重程度呈正相關.
목적 관찰불동충격압력액압충격뇌손상대서신경공능평분차이성,탐토여병이학변화적관계급기림상의의.방법 채용0.1,0.2,0.3 NPa삼충불동타격압력,제작불동충격압력대서액압충격뇌손상모형,관찰생명체정변화、비교사망솔,관찰조모후1h、6h、12h、24h、3d、7d시신경공능평분화뇌수함량,비교기조직학연변추세.결과 응용0.1MPa충격,동물출현일과성호흡감약,사망솔2.08％,신경공능평분최저강지(7.17±0.75)분,단회복교쾌,뇌함수량최고체(81.12±0.03)％,뇌손상부루급충격상처뇌피층적천층;응용0.2MPa충격,동물호흡잠정시간(10.88±2.69)s,사망솔4.17％,신경공능평분최저강지(4.83±0.75)분,뇌수함량최고체(82.74±1.11)％,뇌손상심체해마결구;응용0.3MPa충격,대서호흡잠정시간(20.60±3.02)s,사망솔16.67％,신경공능평분최저강지(2.67±0.52)분,이차회복교만,뇌수함량최고체(83.89±0.04)％,뇌손상심체해마하결구、뇌간.결론 경、중、중삼충불동충격압력액압뇌손상대서신경공능평분의차강저,여기병이학적엄중정도정정상관.
Objective To observe the relationship between neuronal function score and pathological changes of fluid percussion brain injury in rats and to explore their clinical significances.Methods The fluid percussion models of brain injury in rats were established by using the improved device with three kinds impact pressure such as 0.1 MPa,0.2 MPa,0.3 MPa,and vital signs and mortality rate were observed.Behavior changes,brain water content,histological changes were observed by Shapira and Wahld method,dry-wet measure,light microscopy at 1 h,6 h,12 h,24 h,3 d and 7 d after operation respectively.Results The animals accepted impact pressure of 0.1 MPa showed temporary hypopnea with mortality rate of 2.08％,those of 0.2 MPa suffered apnoea of ( 10.88 ±2.69 ) s with mortality rate of 4.17％ and those of 0.3 MPa suffered apnoea of ( 20.60 ± 3.02 ) s with mortality rate of 16.67％.As the impact pressure increased,nervous function score minimumly decreased to (7.17 ±0.75) of 0.1 MPa group,(4.83 ± 0.75 ) of 0.2 MPa group and (2.67 ± 0.52) of 0.3 MPa group respectively,and recovered more slowly.Brain water content maximumly reached to (81.12 ± 0.03 )％,(82.74 ± 1.11 )％ and (83.89± 0.04) ％ at time point of 24 h respectively.The brain injury was involved in the outer layer of cerebral cortex,hippocampal formation and brain stem respectively and histological observation verified above findings.Conclusion Light,moderate and heavy fluid percussion brain injury in rats have more and more low nervous function scores,which have positive relationship with more and more serious pathological changes.
