CAJ | 학술논문

目的研究放射性视网膜病变(Radiation Retinopathy)患者房水中血管内皮生长因子(Vascular Endothelial Growth Factor.VEGF)的浓度,明确其对发病的意义.方法选择2007年3月至2009年3月就诊的放射性视网膜病变患者4例8只眼,增殖性糖尿病性视网膜病变(Proliferative Diabetic Retinopathv,PDR)9名患者10只眼,在行玻璃体腔注入Bevcizumab(1.5mg)治疗前分别抽取100μl前房水;年龄相关性白内障患者10例10只眼,在超声乳化白内障吸除术前,分别抽取lOOμl前房水,所有房水样本均用酶联免疫吸附试验(Enzyme-Linked Immunosorbent Assay,ELISA)检测VEGF浓度.用Kruskal-Wallis H检验对三组患者术前房水中VEGF浓度进行分析,用Mann-Whitney U分别比较三组之间的差异,以P<0.05为差异有统计学意义.结果放射性视网膜病变与PDR患者房水中VEGF浓度无显著性差异,二者均显著高于白内障患者房水中VEGF水平.结论放射性视网膜病变患者房水中VEGF浓度显著升高,VEGF可能是引起放射性视网膜病变的重要因素.
목적 연구방사성시망막병변(Radiation Retinopathy)환자방수중혈관내피생장인자(Vascular Endothelial Growth Factor.VEGF)적농도,명학기대발병적의의.방법 선택2007년3월지2009년3월취진적방사성시망막병변환자4례8지안,증식성당뇨병성시망막병변(Proliferative Diabetic Retinopathv,PDR)9명환자10지안,재행파리체강주입Bevcizumab(1.5mg)치료전분별추취100μl전방수;년령상관성백내장환자10례10지안,재초성유화백내장흡제술전,분별추취lOOμl전방수,소유방수양본균용매련면역흡부시험(Enzyme-Linked Immunosorbent Assay,ELISA)검측VEGF농도.용Kruskal-Wallis H검험대삼조환자술전방수중VEGF농도진행분석,용Mann-Whitney U분별비교삼조지간적차이,이P<0.05위차이유통계학의의.결과 방사성시망막병변여PDR환자방수중VEGF농도무현저성차이,이자균현저고우백내장환자방수중VEGF수평.결론 방사성시망막병변환자방수중VEGF농도현저승고,VEGF가능시인기방사성시망막병변적중요인소.
Objective To investigate VEGF concentration in aqueous humor and its significance to radiation retinopathy. Methods Patients with radiation retinopathy totally 8 eyes of 4 patients and proliferative diabetic retinopathy (PDR) totally 10 eyes of 9 patients from March 2007 to March 2009 came to our hospital were included. Aqueous humor from all these patients was collected before intravitreally injection of Bevacizumab (1.5mg). Ten eyes of 10 age-related cataracts were as controls. Aqueous humor was collected before phacomulcification in cataract patients. VEGF concentrations were detected by enzyme-linked immunosorbent assay (ELISA). VEGF concentrations were compared with Kruskal-Wallis H test among three groups. Mann-Whitney U test was used to compare the differences between two of them. For all statistical tests, P<0.05 was considered significant. Results There was no significant difference between radiation retinopathy and PDR in VEGF concentration. Both of them were much higher than those in cataract patients. Conclusions VEGF concentration elevated significantly in radiation retinopathy. VEGF may play important role in radiation retinopathy.