CAJ | 학술논문

目的观察青蒿琥酯治疗大鼠斯氏狸殖吸虫病后,大鼠肝脏组织病理改变及功能变化.方法将36只清洁级Wistar大鼠随机分成6组.其中5组每只大鼠腹腔注射斯氏狸殖吸虫囊蚴25个,于感染后30 d开始用药治疗,3组为青蒿琥酯治疗组,分别给予青蒿琥酯50 mg/(kg·d)、100 mg/(kg·d)、150 mg/(kg·d),连续给药7 d;吡喹酮组给予150 mg/kg吡喹酮治疗,隔日给药1次,共3次;对照组不做治疗.另设正常对照组.观察大鼠肝脏组织病理改变,摘眼球取血,测定血清中的谷草转氨酶、谷丙转氨酶、白蛋白、球蛋白的含量.结果青蒿琥酯50 mg/(kg·d)、100 mg/(kg·d)与对照组比较,肝脏组织炎症改变较轻,未出现纤维化,青蒿琥酯50 mg/(kg·d)、100 mg/(kg·d)组白蛋白显著升高、球蛋白显著降低、谷丙转氨酶显著降低、白蛋白/球蛋白升高最为明显,与对照组相比差异有统计学意义(P＜0.05).结论一定剂量的青蒿琥酯可减轻斯氏狸殖吸虫病大鼠肝脏的损伤,具有保护肝细胞、降酶作用.
목적 관찰청호호지치료대서사씨리식흡충병후,대서간장조직병리개변급공능변화.방법 장36지청길급Wistar대서수궤분성6조.기중5조매지대서복강주사사씨리식흡충낭유25개,우감염후30 d개시용약치료,3조위청호호지치료조,분별급여청호호지50 mg/(kg·d)、100 mg/(kg·d)、150 mg/(kg·d),련속급약7 d;필규동조급여150 mg/kg필규동치료,격일급약1차,공3차;대조조불주치료.령설정상대조조.관찰대서간장조직병리개변,적안구취혈,측정혈청중적곡초전안매、곡병전안매、백단백、구단백적함량.결과 청호호지50 mg/(kg·d)、100 mg/(kg·d)여대조조비교,간장조직염증개변교경,미출현섬유화,청호호지50 mg/(kg·d)、100 mg/(kg·d)조백단백현저승고、구단백현저강저、곡병전안매현저강저、백단백/구단백승고최위명현,여대조조상비차이유통계학의의(P＜0.05).결론 일정제량적청호호지가감경사씨리식흡충병대서간장적손상,구유보호간세포、강매작용.
Objective To observe the effects of artesunate on hepatic histopathologic change and function of rats infected with Pagumogonimus skrjabini. Methods Thirty-six Wistar rats were randomly divided into 6 groups. Each rat of 5 groups was intra-abdominally injected with 25 metacercaria of P. Skrjabini, 6 rats of one group were remained as normal control. Thirty days after the rats were infected with the metacercaria of P.skrjabini, the intragastric administration was performed in the experimental groups according to following schedule: artesunate 50 mg/(kg·d), 100 mg/(kg·d) and 150 mg/(kg·d), for consecutive 7 d, praziquantel 150 mg/kg q. O. D, for 3 times. Model control group was not treated. The rats were sacrificed for pathological examination with microscope after 30 d. Alanine aminotransferase (ALT)、 aspartate aminotransferase (AST)、albumin (ALB) 、globulin(GLO) were detected in serum by automatic biochemical analyzer. Results Artesunate 50 mg/(kg · d) and 100 mg/(kg·d) treatment could reduce the liver inflammation of the rats infected with the metacercaria of P. Skrjabini. There were significant differences between these two experimental groups and the control group(P ＜ 0.05), on the levels of ALB 、GLO 、A/G、AST in serum. Conclusion Appropriate dose of artesunate may improve the hepatic function of rats infected with P. Skrjabini.