国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2008年
24期
1487-1490
,共4页
王娜%徐琳%张丽%克拉拉·阿巴斯
王娜%徐琳%張麗%剋拉拉·阿巴斯
왕나%서림%장려%극랍랍·아파사
异丙酚%Gq/11蛋白%急性肺损伤%脑%大鼠
異丙酚%Gq/11蛋白%急性肺損傷%腦%大鼠
이병분%Gq/11단백%급성폐손상%뇌%대서
Propofol%Gq/11 protein%Acute lung injury%Brain%Rat
目的 观察异丙酚预处理后Gq/11蛋白在急性肺损伤(acute lung injury,ALI)大鼠脑组织中的动态变化,探讨异丙酚是否通过影响Gq/11蛋白的表达对脑组织起保护作用.方法 用尾静脉注射油酸法复制ALI大鼠模型,将24只健康雄性Wistar大鼠随机分为对照组、ALI组和异丙酚预处理组.检测各组大鼠血气(pH值、PaO2、PaCO2)、平均动脉血压(MABP)以及血浆和脑组织乳酸脱氢酶(LDH)、肌酸激酶(CK)、丙二醛(MDA).Western blot检测各组大鼠脑组织中的Gq/11蛋白含量.结果 异丙酚预处理组Gq/11蛋白含量为(133.89±21.59)%,较对照组增加(33.89±21.59)%(P＜0.01),但低于ALI组[(165.84±30.27)%,P＜0.05].异丙酚预处理组及ALI组PaO2较对照组明显降低(P值均＜0.01),但异丙酚预处理组高于ALI组(P＜0.01).异丙酚预处理组及ALI组MABP均较对照组降低(P值均＜0.01),异丙酚预处理组低于ALI组(P＜0.01).异丙酚预处理组脑组织LDH和CK活性分别为(1.51±0.58)U/mg、(35.83±7.09)U/mg,MDA含量为(4.70±1.59)U/mg,均低于ALI组[分别为(2.38±0.72)U/mg(P＜0.01),(44.31±8.15)U/mg(P＜0.01),(6.78±1.38)U/mg(P＜0.05)].血浆LDH和CK活性、MDA含量呈现与之相似的变化趋势.结论 异丙酚预处理能减轻ALI时脑的损伤程度,其保护作用可能与下调Gq/11蛋白有关.
目的 觀察異丙酚預處理後Gq/11蛋白在急性肺損傷(acute lung injury,ALI)大鼠腦組織中的動態變化,探討異丙酚是否通過影響Gq/11蛋白的錶達對腦組織起保護作用.方法 用尾靜脈註射油痠法複製ALI大鼠模型,將24隻健康雄性Wistar大鼠隨機分為對照組、ALI組和異丙酚預處理組.檢測各組大鼠血氣(pH值、PaO2、PaCO2)、平均動脈血壓(MABP)以及血漿和腦組織乳痠脫氫酶(LDH)、肌痠激酶(CK)、丙二醛(MDA).Western blot檢測各組大鼠腦組織中的Gq/11蛋白含量.結果 異丙酚預處理組Gq/11蛋白含量為(133.89±21.59)%,較對照組增加(33.89±21.59)%(P＜0.01),但低于ALI組[(165.84±30.27)%,P＜0.05].異丙酚預處理組及ALI組PaO2較對照組明顯降低(P值均＜0.01),但異丙酚預處理組高于ALI組(P＜0.01).異丙酚預處理組及ALI組MABP均較對照組降低(P值均＜0.01),異丙酚預處理組低于ALI組(P＜0.01).異丙酚預處理組腦組織LDH和CK活性分彆為(1.51±0.58)U/mg、(35.83±7.09)U/mg,MDA含量為(4.70±1.59)U/mg,均低于ALI組[分彆為(2.38±0.72)U/mg(P＜0.01),(44.31±8.15)U/mg(P＜0.01),(6.78±1.38)U/mg(P＜0.05)].血漿LDH和CK活性、MDA含量呈現與之相似的變化趨勢.結論 異丙酚預處理能減輕ALI時腦的損傷程度,其保護作用可能與下調Gq/11蛋白有關.
목적 관찰이병분예처리후Gq/11단백재급성폐손상(acute lung injury,ALI)대서뇌조직중적동태변화,탐토이병분시부통과영향Gq/11단백적표체대뇌조직기보호작용.방법 용미정맥주사유산법복제ALI대서모형,장24지건강웅성Wistar대서수궤분위대조조、ALI조화이병분예처리조.검측각조대서혈기(pH치、PaO2、PaCO2)、평균동맥혈압(MABP)이급혈장화뇌조직유산탈경매(LDH)、기산격매(CK)、병이철(MDA).Western blot검측각조대서뇌조직중적Gq/11단백함량.결과 이병분예처리조Gq/11단백함량위(133.89±21.59)%,교대조조증가(33.89±21.59)%(P＜0.01),단저우ALI조[(165.84±30.27)%,P＜0.05].이병분예처리조급ALI조PaO2교대조조명현강저(P치균＜0.01),단이병분예처리조고우ALI조(P＜0.01).이병분예처리조급ALI조MABP균교대조조강저(P치균＜0.01),이병분예처리조저우ALI조(P＜0.01).이병분예처리조뇌조직LDH화CK활성분별위(1.51±0.58)U/mg、(35.83±7.09)U/mg,MDA함량위(4.70±1.59)U/mg,균저우ALI조[분별위(2.38±0.72)U/mg(P＜0.01),(44.31±8.15)U/mg(P＜0.01),(6.78±1.38)U/mg(P＜0.05)].혈장LDH화CK활성、MDA함량정현여지상사적변화추세.결론 이병분예처리능감경ALI시뇌적손상정도,기보호작용가능여하조Gq/11단백유관.
Objective To observe the alteration of Gq/11 protein expression in the brain of rats with acute lung injury(ALI)after propofol pre-treatment and to explore whether it plays a protective role in brain injury during ALI.Methods ALI rat model was reproduced by intravenous injection of oleic acid.Twentyfour male Wister rats were randomly divided into three groups:control group,ALI group and propofol group.Blood gas indexes(pH,PaO2,PaCO2),mean arterial blood pressure(MABP)and MDA,LDH,CK of plasma and brain were measured.Gq/11 protein concentration was examined by Western blot.Results Gq/11protein concentration in the brain of propofol group[(133.89±21.59)%]was increased compared with control group(P＜0.01),and it was decreased compared with ALI group[(165.84±30.27)%,P＜0.053.PaO2 of propofol groUp and ALI group were lower than that of control group(all P＜0.01),and PaO2 of propofol group was higher than that of ALI group(P＜0.01).MABP of propofol group and ALI group obviously reduced(all P＜0.01),and MABP of propofol group was lower than that of ALI group(P＜0.01).LDH activity[(1.51±0.58)U/mg],CK activity[(35.83±7.09)U/mg]and MDA content[(4.70±1.59)U/mg]in homogenato of propofol group were lower than those in ALI group[(2.38±0.72)U/mg(P＜0.01),(44.31±8.15)U/mg(P＜0.01),(6.78±1.38)U/mg(P＜0.05)].The levels of LDH,CK and MDA in plasma were same as in brain.Conclusions Pre-treatment with propofol can relieve the harm,which has some relation with reducing Gq/11 protein concentration.
