CAJ | 학술논문

目的探讨二氢青蒿素诱导类风湿关节炎滑膜细胞凋亡的信号机制.方法无菌条件取类风湿关节炎患者膝关节滑膜组织,组织块贴壁法培养关节滑膜细胞.流式细胞仪检测细胞凋亡.Westernblot半定量分析Akt活化.电泳迁移率变动(EM-SA)检测NF-κB活性.结果在2.5～10μmol/L质量浓度范围二氢青蒿素呈剂量依赖性诱导体外培养的关节炎滑膜细胞凋亡.5μmoL/L和10μmoL/L的二氢青蒿素与关节滑膜细胞共培养24 h显著抑制Akt激酶473位丝氨酸磷酸化及NF-κB的活化.结论二氢青蒿素通过Akt信号途径诱导类风湿关节炎滑膜细胞凋亡.
목적 탐토이경청호소유도류풍습관절염활막세포조망적신호궤제.방법 무균조건취류풍습관절염환자슬관절활막조직,조직괴첩벽법배양관절활막세포.류식세포의검측세포조망.Westernblot반정량분석Akt활화.전영천이솔변동(EM-SA)검측NF-κB활성.결과 재2.5～10μmol/L질량농도범위이경청호소정제량의뢰성유도체외배양적관절염활막세포조망.5μmoL/L화10μmoL/L적이경청호소여관절활막세포공배양24 h현저억제Akt격매473위사안산린산화급NF-κB적활화.결론 이경청호소통과Akt신호도경유도류풍습관절염활막세포조망.
Objective To explore the mechanism of apeptosis in rheumatoid arthritis synoviocyte induced by dihydroartemisinin. Methods Synovial tissues were cut from rheumatoid arthritis patients when who was under knee prosthesis. Apoptosis was detected with flow cytometry. Western blot was performed to assess ser473-phosphorylated Akt. EMSA (electrophoretic mobility shift assay) was used to ana-lyze NF-κB activation. Results Dihydroartemisin can induce apoptosis in rheumatoid arthritis synoviocyte in a dose-dependent manor from 2.5μmol/L to 10μmol/L. Rheumatoid Arthritis synoviocyte cultured with dihydroartemisinin in 5μmol/L or 10μmoL/L can significantly in-hibit serine 473 phosphorylation in Akt and activation of NF-κB. Conclusion Dihydroartemisinin can induce apoptosis in rheumatoid arthri-tis synoviocyte through Akt signal pathway.