CAJ | 학술논문

目的研究端粒酶活性和相关基因表达在白血病患者中的变化及其在白血病发病机制中的意义。[ HTH 方法通过半定量端粒重复序列扩增（TRAP）-银染和RT-PCR方法检测了47 例白血病患者骨髓细胞的端粒酶活性和相关基因hEST2、TP1、h TR mRNA表达水平。结果 42例白血病进展阶段标本的端粒酶活性和相关基因表达水平高于急性白血病完全缓解期、慢性粒细胞白血病(慢粒)慢性期、正常和贫血标本（P<0.01）；14例急性白血病完全缓解期和(或)慢粒慢性期标本端粒酶活性和TP1、hTR基因表达水平高于正常和贫血标本（P<0.05）；在部分正常和贫血标本中检测到相对低水平的端粒酶活性和相关基因表达，3例贫血标本经短期常规培养后检测到端粒酶活性和基因表达增高。结论端粒酶活性和相关基因表达异常升高是造血细胞恶性转化的特异性标记之一，检测端粒酶活性和相关基因表达有助于白血病患者的疗效观察。正常骨髓的端粒酶活性与具有增殖分化能力的造血干细胞有关。hE ST2可能是端粒酶的正调控结构基因，hTR基因表达与端粒酶的反馈调控机制有关。
목적연구단립매활성화상관기인표체재백혈병환자중적변화급기재백혈병발병궤제중적의의。[ HTH 방법통과반정량단립중복서렬확증（TRAP）-은염화RT-PCR방법검측료47 례백혈병환자골수세포적단립매활성화상관기인hEST2、TP1、h TR mRNA표체수평。결과 42례백혈병진전계단표본적단립매활성화상관기인표체수평고우급성백혈병완전완해기、만성립세포백혈병(만립)만성기、정상화빈혈표본（P<0.01）；14례급성백혈병완전완해기화(혹)만립만성기표본단립매활성화TP1、hTR기인표체수평고우정상화빈혈표본（P<0.05）；재부분정상화빈혈표본중검측도상대저수평적단립매활성화상관기인표체，3례빈혈표본경단기상규배양후검측도단립매활성화기인표체증고。결론단립매활성화상관기인표체이상승고시조혈세포악성전화적특이성표기지일，검측단립매활성화상관기인표체유조우백혈병환자적료효관찰。정상골수적단립매활성여구유증식분화능력적조혈간세포유관。hE ST2가능시단립매적정조공결구기인，hTR기인표체여단립매적반궤조공궤제유관。
Objective　To investigate the changes of telomerase activity and its related genes expressi on in leukemia cells and their roles in leukemogenesis. Methods　T he telomerase activity in bone marrow cells of 47 leukemia patients were detecte d by a semi-quantitative telomeric repeat amplification protocol (TRAP)-silver staining, and the expression of telomerase ralated genes hEST2, TP1 and hTR by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). [ WTHZ Results　The telomerase activity and expression of telomerase rela ted genes were significantly higher in 42 leukemia patients in progress stage th an that in complete remission/chronic stage and in normal/anemia cases (P[ WTBZ]<0.01).The telomerase activity and expression of TP1,hTR gene in 14 cases of acute leuk emia complete remission (AL-CR)/chronic myelogenous (CML)-chronic stage were h igher than that in normal/anemia cases (P<0.05). Low levels of telom erase activi ty and telomerase related genes expression could be detected in part of normal b one marrow cells. The levels of telomerase activity and telomerase related genes expression in 3 cases of anemia increased after short-term culture. Conc lusions　High level of telomerase activity and its related genes express ion were specific markers of malignant hematopoietic cells and their changes mig ht be useful for observation of treatment effectiveness in leukemia. Low level of telomerase activity was associated with hematopoietic stem cells with the abi lity of proliferation. hEST2 gene might be a up-regulation gene of telomerase; hTR might be related to feedback regulation of telomerase.