中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2011年
1期
24-28
,共5页
孙晓阳%丁涟沭%金孝东%卜向飞%蒋健%李正明%卞爱苗%王晓东%刘岱%刘骥%惠晓波%王彦平%朱波
孫曉暘%丁漣沭%金孝東%蔔嚮飛%蔣健%李正明%卞愛苗%王曉東%劉岱%劉驥%惠曉波%王彥平%硃波
손효양%정련술%금효동%복향비%장건%리정명%변애묘%왕효동%류대%류기%혜효파%왕언평%주파
神经胶质瘤%病理学%信号传导%预后
神經膠質瘤%病理學%信號傳導%預後
신경효질류%병이학%신호전도%예후
Glioma%Clinical pathology%Signal transduction pathway%Prognosis
目的 探讨磷脂酰肌醇3-激酶(PI3K),磷酸化蛋白激酶B(p-AKT)和磷酸化哺乳动物雷帕霉素靶蛋白(p-mTOR)在人脑胶质瘤组织中的表达及其与人脑胶质瘤恶性进展和预后的相关性.方法 选取南京医科大学附属淮安第一医院神经外科自2004年9月至2008年9月间手术切除并经病理证实的人脑胶质瘤标本88例,另取非肿瘤组织中的正常脑组织标本20例作为对照.采用免疫组织化学染色检测脑胶质瘤组织和正常脑组织中PI3K、p-AKT及p-mTOR的表达,并统计分析其与患者的临床病理学特征及预后的关系.结果 PI3K、p-AKT、p-mTOR在脑胶质瘤组织中的阳性表达率均显著高于正常脑组织,差异有统计学意义(PI3K:x2=14.028,P=0.009;p-AKT:x2=15.132,P=0.008和mTOR:x2=15.293,P=0.008);不同病理分级、治疗前KPS评分以及临床分期脑胶质瘤组织中PI3K、p-AKT和p-mTOR阳性表达率的差异均有统计学意义(P＜0.05);PI3K、p-AKT、p-mTOR阳性表达组患者的5年总体生存率均显著低于其阴性表达组(PI3K:x2=8.381,P=0.026;p-AKT:x2=12.923,P=0.011;mTOR:x2=13.252,P=0.013).结论 PI3K/Akt/mTOR信号传导通路在脑胶质瘤组织中被过度激活,与肿瘤的恶性程度密切相关,可以作为判断脑胶质瘤患者预后的生物学指标.
目的 探討燐脂酰肌醇3-激酶(PI3K),燐痠化蛋白激酶B(p-AKT)和燐痠化哺乳動物雷帕黴素靶蛋白(p-mTOR)在人腦膠質瘤組織中的錶達及其與人腦膠質瘤噁性進展和預後的相關性.方法 選取南京醫科大學附屬淮安第一醫院神經外科自2004年9月至2008年9月間手術切除併經病理證實的人腦膠質瘤標本88例,另取非腫瘤組織中的正常腦組織標本20例作為對照.採用免疫組織化學染色檢測腦膠質瘤組織和正常腦組織中PI3K、p-AKT及p-mTOR的錶達,併統計分析其與患者的臨床病理學特徵及預後的關繫.結果 PI3K、p-AKT、p-mTOR在腦膠質瘤組織中的暘性錶達率均顯著高于正常腦組織,差異有統計學意義(PI3K:x2=14.028,P=0.009;p-AKT:x2=15.132,P=0.008和mTOR:x2=15.293,P=0.008);不同病理分級、治療前KPS評分以及臨床分期腦膠質瘤組織中PI3K、p-AKT和p-mTOR暘性錶達率的差異均有統計學意義(P＜0.05);PI3K、p-AKT、p-mTOR暘性錶達組患者的5年總體生存率均顯著低于其陰性錶達組(PI3K:x2=8.381,P=0.026;p-AKT:x2=12.923,P=0.011;mTOR:x2=13.252,P=0.013).結論 PI3K/Akt/mTOR信號傳導通路在腦膠質瘤組織中被過度激活,與腫瘤的噁性程度密切相關,可以作為判斷腦膠質瘤患者預後的生物學指標.
목적 탐토린지선기순3-격매(PI3K),린산화단백격매B(p-AKT)화린산화포유동물뢰파매소파단백(p-mTOR)재인뇌효질류조직중적표체급기여인뇌효질류악성진전화예후적상관성.방법 선취남경의과대학부속회안제일의원신경외과자2004년9월지2008년9월간수술절제병경병리증실적인뇌효질류표본88례,령취비종류조직중적정상뇌조직표본20례작위대조.채용면역조직화학염색검측뇌효질류조직화정상뇌조직중PI3K、p-AKT급p-mTOR적표체,병통계분석기여환자적림상병이학특정급예후적관계.결과 PI3K、p-AKT、p-mTOR재뇌효질류조직중적양성표체솔균현저고우정상뇌조직,차이유통계학의의(PI3K:x2=14.028,P=0.009;p-AKT:x2=15.132,P=0.008화mTOR:x2=15.293,P=0.008);불동병리분급、치료전KPS평분이급림상분기뇌효질류조직중PI3K、p-AKT화p-mTOR양성표체솔적차이균유통계학의의(P＜0.05);PI3K、p-AKT、p-mTOR양성표체조환자적5년총체생존솔균현저저우기음성표체조(PI3K:x2=8.381,P=0.026;p-AKT:x2=12.923,P=0.011;mTOR:x2=13.252,P=0.013).결론 PI3K/Akt/mTOR신호전도통로재뇌효질류조직중피과도격활,여종류적악성정도밀절상관,가이작위판단뇌효질류환자예후적생물학지표.
Objective To investigate the protein expression of phosphatidylinositol 3-kinase (PI3K), phosphorylated Akt B (p-Akt) and p-mTOR in human gliomas, and evaluate their clinical significance in clinicopathological status and prognosis of these patients with gliomas. Methods Eighty-eight patients, admitted to our hospital from September 2004 to September 2008, were chosen in our study; these patients were performed surgical resection and the samples were pathologically confirmed as gliomas. Another 20 samples, cut from the normal brain tissue were adopted as controls.Immunohistochemistry was employed to examine the protein expression of PI3K, p-AKT and p-mTOR.Then, the correlation of their expression with the clinicopathological features of the gliomas and prognosis of the patients was further analyzed. Results The positive expression rates of PI3K in gliomas and normal brain tissues were 68.18% (60/88) and 18.18% (16/88), respectively; those of p-AKT were 73.86% (65/88) and 17.05% (15/88), respectively;, those of p-mTOR were 75.00% (66/88) and 18.18% (16/88), respectively; the expression levels of these 3 proteins were all significantly higher than those in normal brain tissues (PI3K: x2=14.028, P=0.009; p-AKT: x2=15.132, P=0.008 and mTOR:x2=15.293, P=0.008). The positive expression rates of PI3K, p-AKT and p-mTOR were significantly different in the gliomas with pathological grades, different scores of Karnofsky performance status and different clinical stages (P＜0.05). In addition, the 5-year overall survival rate in PI3K-positive group,p-AKT-positive group and p-mTOR-positive group was significantly lower than in those negative groups (PI3K: x2=8.381, P=0.026; p-AKT: x2=12.923, P=0.011; mTOR: x2=13.252, P=0.013). Conclusion PI3K/Akt/mTOR signal transduction pathway is over-activated in gliornas, which is closely correlated to the grade-malignancy; and the positive expression of PI3K, p-AKT and p-mTOR may predict the poor prognosis of the patients with gliomas.
