中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
11期
1651-1653
,共3页
齐云秋%刘宏建%潘芦羚%程敬亮
齊雲鞦%劉宏建%潘蘆羚%程敬亮
제운추%류굉건%반호령%정경량
熊果酸%骨肉瘤%骨形态发生蛋白2%骨形态发生蛋白6
熊果痠%骨肉瘤%骨形態髮生蛋白2%骨形態髮生蛋白6
웅과산%골육류%골형태발생단백2%골형태발생단백6
Ursolic acid%Osteosareoma%BMP-2%BMP-6
目的 探讨熊果酸(UA)对人骨肉瘤MG63细胞的影响作用及其机制.方法 体外培养骨肉瘤MG63细胞,应用浓度为10、20、50、100 μmol/L的熊果酸干预骨肉瘤MG63细胞,噻唑蓝(MTT)比色法检测细胞的增殖,逆转录-聚合酶链反应(RT-PCR)技术检测细胞中骨形态发生蛋白(BMP)-2和BMP-6的mRNA的表达,并应用蛋白印记技术检测相应蛋白的表达水平.结果 熊果酸干预组的肿瘤细胞增殖被明显抑制,72 h时100 μmol/L组的增殖活性为(0.25±0.03),而10μmol/L组的增殖活性为(0.86±0.10),差异有统计学意义(P＜0.05),各浓度组在24、48、72 h时的增殖活性差异均有统计学意义(P＜0.05);BMP-2和BMP-6 mRNA和蛋白的表达水平在实验组和对照组间差异均有统计学意义(P＜0.05),而两对照组之间差异无统计学意义(P＞0.05).结论 熊果酸能够抑制骨肉瘤MG63细胞的增殖,并能够促进细胞中BMP-2和BMP-6的表达.
目的 探討熊果痠(UA)對人骨肉瘤MG63細胞的影響作用及其機製.方法 體外培養骨肉瘤MG63細胞,應用濃度為10、20、50、100 μmol/L的熊果痠榦預骨肉瘤MG63細胞,噻唑藍(MTT)比色法檢測細胞的增殖,逆轉錄-聚閤酶鏈反應(RT-PCR)技術檢測細胞中骨形態髮生蛋白(BMP)-2和BMP-6的mRNA的錶達,併應用蛋白印記技術檢測相應蛋白的錶達水平.結果 熊果痠榦預組的腫瘤細胞增殖被明顯抑製,72 h時100 μmol/L組的增殖活性為(0.25±0.03),而10μmol/L組的增殖活性為(0.86±0.10),差異有統計學意義(P＜0.05),各濃度組在24、48、72 h時的增殖活性差異均有統計學意義(P＜0.05);BMP-2和BMP-6 mRNA和蛋白的錶達水平在實驗組和對照組間差異均有統計學意義(P＜0.05),而兩對照組之間差異無統計學意義(P＞0.05).結論 熊果痠能夠抑製骨肉瘤MG63細胞的增殖,併能夠促進細胞中BMP-2和BMP-6的錶達.
목적 탐토웅과산(UA)대인골육류MG63세포적영향작용급기궤제.방법 체외배양골육류MG63세포,응용농도위10、20、50、100 μmol/L적웅과산간예골육류MG63세포,새서람(MTT)비색법검측세포적증식,역전록-취합매련반응(RT-PCR)기술검측세포중골형태발생단백(BMP)-2화BMP-6적mRNA적표체,병응용단백인기기술검측상응단백적표체수평.결과 웅과산간예조적종류세포증식피명현억제,72 h시100 μmol/L조적증식활성위(0.25±0.03),이10μmol/L조적증식활성위(0.86±0.10),차이유통계학의의(P＜0.05),각농도조재24、48、72 h시적증식활성차이균유통계학의의(P＜0.05);BMP-2화BMP-6 mRNA화단백적표체수평재실험조화대조조간차이균유통계학의의(P＜0.05),이량대조조지간차이무통계학의의(P＞0.05).결론 웅과산능구억제골육류MG63세포적증식,병능구촉진세포중BMP-2화BMP-6적표체.
Objective To study the effects of Ursolic Acid (UA) on the Human MC63 osteosareoma cell. Methods Human MC63 osteosareoma cells were cultured in vitro,and the concentrations 10,20,50,100 μmol/L of ursolic acid were added into culture fluid. Cell proliferation were measured by methyl thiazol tetrazolium (MTT), the expression of mRNA levels of bone morphogenetic protein (BMP)-2 and BMP-6 were detected by reverse transcription-polymerase chain reaction (RT-PCR). And the expression of proteins were detected by Western boltting. Results Compared with the blank group, the proliferation in experiment group was suppressed with time and concentration dependent. The proliferation was 0. 25 ±0. 03in 100 μmol/L group while it was 0. 86 ± 0. 10 in 10 μmol/L group after 72 h ( P ＜ 0. 05 ). The proliferation was statistical significance after 24,48,72 h in different concentrations. The expressions of BMP-2 and BMP-6 were higher than that in blank group, and there was statistical significance ( P ＜ 0. 05 ). But there was no statistical significance between two experiment groups (P ＞ 0. 05 ). Conclusion Ursolic acid can suppressed the proliferation of MG63 osteosareoma cells with time and concentration dependent. promoted,which has relation with promoting the expressions of BMP-2 and BMP-6.
