CAJ | 학술논문

目的观察细胞s期激酶相关蛋白2(SKP2)、p16蛋白在非小细胞肺癌(NSCLC)组织中的表达,探讨它们之间的关系及其临床意义.方法利用组织芯片和免疫组织化学技术检测SKP2、p16蛋白在89例非小细胞肺癌,10例肺良性病变组织中的表达.结果 NSCLC组织中SKP2、p16蛋白表达阳性率分别为(23.52±13.57)%、(44.72±15.97)%,均与肺良性病变组织差异有统计学意义(2.91±1.27)%、(91.13±6.57)%(P＜0.01).SKP2蛋白在NSCLC组织中的表达水平与细胞分化程度,病理类型,TNM分期密切相关(P＜0.01),而与淋巴结转移差异无统计学意义(P＞0.05).p16蛋白在NSCLC组织中的表达水平与细胞分化程度,病理类型,TNM分期,淋巴结转移密切相关(P＜0.01).NSCLC组织中SKP2、p16蛋白表达呈负相关(r=-0.309,P＜0.01).NSCLC组织中SKP2蛋白表达率与患者生存时间呈负相关(r=-0.241,P＜0.01),p16蛋白表达率与患者生存时间呈负正相关(r=0.144,P＜0.01).结论 SKP2蛋白表达增高与p16蛋白表达降低共同在NSCLC的发生发展中起促进作用,且它们对NSCLC患者的预后也有相反的影响.
목적 관찰세포s기격매상관단백2(SKP2)、p16단백재비소세포폐암(NSCLC)조직중적표체,탐토타문지간적관계급기림상의의.방법 이용조직심편화면역조직화학기술검측SKP2、p16단백재89례비소세포폐암,10례폐량성병변조직중적표체.결과 NSCLC조직중SKP2、p16단백표체양성솔분별위(23.52±13.57)%、(44.72±15.97)%,균여폐량성병변조직차이유통계학의의(2.91±1.27)%、(91.13±6.57)%(P＜0.01).SKP2단백재NSCLC조직중적표체수평여세포분화정도,병리류형,TNM분기밀절상관(P＜0.01),이여림파결전이차이무통계학의의(P＞0.05).p16단백재NSCLC조직중적표체수평여세포분화정도,병리류형,TNM분기,림파결전이밀절상관(P＜0.01).NSCLC조직중SKP2、p16단백표체정부상관(r=-0.309,P＜0.01).NSCLC조직중SKP2단백표체솔여환자생존시간정부상관(r=-0.241,P＜0.01),p16단백표체솔여환자생존시간정부정상관(r=0.144,P＜0.01).결론 SKP2단백표체증고여p16단백표체강저공동재NSCLC적발생발전중기촉진작용,차타문대NSCLC환자적예후야유상반적영향.
Ohiective To research the expressive characteristics of SKP2 and p16 protein in nonsmall cell lung carcinoma(NSCLC)and their affection to NSCLC patients' prognosis.Methods The expression of SKP2 and p16 protein was detected in 89 NSCLC,10 lung benign lesion tissues by Tissue Chip and Immuno-histochemistry technology.Results The positive rate of SKP2 and p16 protein stain were (23.52±13.57)%and(44.72±15.97)% in NSCLC tissues,which were significantly different with lung benign lesion tissues(2.91±1.27)%and(91.13±6.57)%(P＜0.01).The expressive level of SKP2 protein in NSCLC tissues was closely related to cell differentiation,pathological type,TNM stage(P＜0.01),but not to lymph node metastasis(P＞0.05).The expressive level of p16 protein in NSCLC tissues was closely related to cell differentiation,pathological type,TNM stage,lymph node metastasis(P=0.01).The expression of SKP2 protein was negative correlation with p16 protein in NSCLC(r=-0.309,P＜0.01).The expression rate of SKP2 protein in NSCLC was negative correlation with patients' lifetime (r=-0.241,P＜0.01).The expression rate of p16 protein in NSCLC was positive correlation with patients' lifetime(r=0.144,P＜0.01).Conclusion High expression of SKP2 protein and low expression of p16 protein concurrently play a facilitative role in occurrence and development of NSCLC.Moreover,there was a certain opposite role to NSCLC patients' prognosis,Yet.it needs farther research that the interactive mechanism between them.