CAJ | 학술논문

目的探讨Apelin在胃癌发生、发展中的意义及其与微血管密度(MVD)的相互关系.方法应用免疫组织化学SP法检测20例正常胃黏膜(距肿瘤边缘5 cm)、20例中重度不典型增生和60例胃腺癌组织中Apelin表达情况.用CD34标记免疫组织化学SP法检测MVD.结果 Apelin在正常胃黏膜、中重度不典型增生组织和胃癌组织中阳性表达率分别为5.0%(1/20)、15.0%(3/20)和83.3%(50/60),Apelin随着胃癌的发展阳性率逐渐增高,且差异有统计学意义(P<0.05);Apelin表达与胃癌患者TNM分期及分化程度有关;Apelin表达阴性组与阳性组间MVD值分别为200倍视里下(38.90±10.21)个和(47.01±11.66)个,MVD值在Apelin阳性表达组高于阴性组,差异有统计学意义(P<0.01);MVD表达与肿瘤浆膜浸润(P<0.05)、淋巴结转移有关(P<0.05).结论 Apelin可能通过促进肿瘤血管生成参与胃癌的发生与发展.
목적 탐토Apelin재위암발생、발전중적의의급기여미혈관밀도(MVD)적상호관계.방법 응용면역조직화학SP법검측20례정상위점막(거종류변연5 cm)、20례중중도불전형증생화60례위선암조직중Apelin표체정황.용CD34표기면역조직화학SP법검측MVD.결과 Apelin재정상위점막、중중도불전형증생조직화위암조직중양성표체솔분별위5.0%(1/20)、15.0%(3/20)화83.3%(50/60),Apelin수착위암적발전양성솔축점증고,차차이유통계학의의(P<0.05);Apelin표체여위암환자TNM분기급분화정도유관;Apelin표체음성조여양성조간MVD치분별위200배시리하(38.90±10.21)개화(47.01±11.66)개,MVD치재Apelin양성표체조고우음성조,차이유통계학의의(P<0.01);MVD표체여종류장막침윤(P<0.05)、림파결전이유관(P<0.05).결론 Apelin가능통과촉진종류혈관생성삼여위암적발생여발전.
Objective To explore the roles of Apelin in the development of gastric carcinoma (GC) and interrelation with MVD. Methods Immunohistochemical streptacidin/peroxidase (SP) technique was adopted to examine the expressions of Apelin in 20 cases of normal gastric mucosa, 20 cases of atypical hyperplasia (Dys) and 60 cases of gastric carcinoma, and to examine MVD by CD34 staining. Results With the development of GC, the positive rate of Apelin was increased gradually, namely 5.0 %(1/20) in normal gastric mucosa group, 15.0 %(3/20) in atypical hyperplasia group and 83.3 %(50/60) in gastric carcinoma. Expression of Apelin was correlated with TNM stage and differentiation. MVD in the Apelin positive group (47.01±11.66) was significantly higher than that in the negative group(38.90±10.21); MVD of the Apelin positive group expression was correlated with the histologic stage, serosa infiltration and lymph node metastasis (P < 0.05). Conclusion Apelin may contribute to the development of GC by activating tumour neoangiogenesis.