CAJ | 학술논문

目的建立快速、有效的反相高效液相(RP-HPLC)检测方法,测定不同品种和产地红景天中红景天苷、红景天芬、酪醇、红景天素、红景天任的含量,以评价红景天的质量.方法方法Ⅰ:甲醇-水(0.5mmol/L SDS的1%醋酸水溶液)检测红景天苷;方法Ⅱ:乙腈-水检测红景天芬;方法Ⅲ:乙腈-磷酸水溶液梯度洗脱同时检测5个有效成分.结果红景天苷的含量为0.021%～1.420%;红景天芬除在蔷薇红景天和高山红景天中含量较高外,在其他品种中均非常低或低于检测限;5个有效成分的含量因品种或产地的不同而有很大的变化.结论建立的3种RP-HPLC方法可同时测定红景天苷、红景天芬等5个有效成分.综合考虑不同品种中5个有效成分的含量,以产自新疆的蔷薇红景天和吉林的高山红景天为国内较好的品种.
목적건립쾌속、유효적반상고효액상(RP-HPLC)검측방법,측정불동품충화산지홍경천중홍경천감、홍경천분、락순、홍경천소、홍경천임적함량,이평개홍경천적질량.방법방법Ⅰ:갑순-수(0.5mmol/L SDS적1%작산수용액)검측홍경천감;방법Ⅱ:을정-수검측홍경천분;방법Ⅲ:을정-린산수용액제도세탈동시검측5개유효성분.결과홍경천감적함량위0.021%～1.420%;홍경천분제재장미홍경천화고산홍경천중함량교고외,재기타품충중균비상저혹저우검측한;5개유효성분적함량인품충혹산지적불동이유흔대적변화.결론건립적3충RP-HPLC방법가동시측정홍경천감、홍경천분등5개유효성분.종합고필불동품충중5개유효성분적함량,이산자신강적장미홍경천화길림적고산홍경천위국내교호적품충.
Object To develop the reliable RP-HPLC methods for the determination of salidroside,tyrosol,rosavin,rosin,and rosarin in the plants of Rhodiola L.and to evaluate their species from different habitats.Methods Method Ⅰ :methanol-water(0.5 mmol/L SDS in 1% acetic acid aqueous solution)system for the analysis of salidroside;method ⅡⅡ:acetonitrile-water system for rosavin;method Ⅲ:aqueous acetonitrile-phosphoric gradient system for salidroside,tyrosol,rosavin,rosin,and rosarin.Results The contents of salidroside in different species range from 0.021% to 1.420%,and those of rosavin in all species are very limited or undetected except in Rhodiola rosea L.and R.sachalinensis.The contents of the five marker ingredients are significantly species- and habitat-dependent.Conclusion Three RP-HPLC methods are established for quantitative analysis of the above five marker ingredients in the meantime,respectively.Evaluation of the quality of varied species of Rhodiola L.shows that R.rosea growing in Xinjiang Uygur Autonomous Region and R.sachalinensis growing in Jilin province are the two better species contained with abundant above-mentioned ingredients in China.