CAJ | 학술논문

背景:脑震荡是一种轻型颅脑损伤,因其客观指标较少,常为临床诊断和治疗带来难度.在基础研究方面,脑啡肽和多巴胺在其中的表达及其意义尚不清楚.目的:探讨神经元型一氧化氮合酶(neuron nitric oxide synthase,nNOS)在大鼠实验性脑震荡中的表达及意义.设计:随机对照实验研究.地点和对象:实验地点:解放军军事医学科学院放射医学研究所.健康Wistar雄性二级(清洁级)大鼠80只,军事医学科学院实验动物中心清洁级动物房饲养,水料任意,用于复制脑震荡动物模型,依致脑震荡所用砝码质量不同,随机分为对照组,50,100,200 g组.主要观察指标:实验动物于伤后1,3,7,14及30 d活杀取脑组织,经免疫组化和原位杂交等技术研究nNOS在脑震荡中的变化规律.结果:100 g组见典型脑震荡的临床表现,其病理改变为脑血管扩张,脑组织瘀血、水肿,神经元变性、坏死,尼氏体减少甚至消失.nNOS蛋白和mRNA于伤后3 d表达增强,7 d达高峰,14 d后开始减少,30 d仍呈阳性表达.阳性部位见于大脑皮质、海马、丘脑和小脑神经元胞浆内.结论:脑震荡以血液循环障碍和实质细胞变性、坏死为主要病理改变;nNOS基因表达参与脑震荡发生时脑组织损伤的病理过程,可能对神经细胞变性、坏死起重要调节作用.
배경:뇌진탕시일충경형로뇌손상,인기객관지표교소,상위림상진단화치료대래난도.재기출연구방면,뇌배태화다파알재기중적표체급기의의상불청초.목적:탐토신경원형일양화담합매(neuron nitric oxide synthase,nNOS)재대서실험성뇌진탕중적표체급의의.설계:수궤대조실험연구.지점화대상:실험지점:해방군군사의학과학원방사의학연구소.건강Wistar웅성이급(청길급)대서80지,군사의학과학원실험동물중심청길급동물방사양,수료임의,용우복제뇌진탕동물모형,의치뇌진탕소용겁마질량불동,수궤분위대조조,50,100,200 g조.주요관찰지표:실험동물우상후1,3,7,14급30 d활살취뇌조직,경면역조화화원위잡교등기술연구nNOS재뇌진탕중적변화규률.결과:100 g조견전형뇌진탕적림상표현,기병리개변위뇌혈관확장,뇌조직어혈、수종,신경원변성、배사,니씨체감소심지소실.nNOS단백화mRNA우상후3 d표체증강,7 d체고봉,14 d후개시감소,30 d잉정양성표체.양성부위견우대뇌피질、해마、구뇌화소뇌신경원포장내.결론:뇌진탕이혈액순배장애화실질세포변성、배사위주요병리개변;nNOS기인표체삼여뇌진탕발생시뇌조직손상적병리과정,가능대신경세포변성、배사기중요조절작용.
BACKGROUND: Cerebral concussion is a mild brain injury. In basic researches, the expression and significance of enkaphalin and dopamine in cerebral concussion remain poorly understood.OBJECTIVE: To observe the expression of neuronal nitric oxide synthase (nNOS) gene in rat models of cerebral concussion and to explore its significance.DESIGN: A randomized controlled trialled study.SETTING and PARTICIPANTS: This study was conducted in the Institute of Radiation Medicine, Academy of Military Medical Sciences. Rat models of cerebral concussion was established in 80 healthy male Wistar rats of clean grade purchased from the Experimental Animal Center of Academy of Military Medical Sciences with free access to food and water. The rats were randomly divided into 4 groups according to the different levels of cerebral impact for model establishment, namely the control group, 50, 100 and 200 g counterweight groups.MAIN OUTCOME MEASURES: Brain tissue samples were taken 1, 3, 7,14 and 30 days after injury respectively, from each group, to examine the changes in the expression of nNOS gene in the course of cerebral concussion by means of immunohistochemistry and in situ hybridization.RESULTS: Rats in 100 g group exhibited typical manifestations of cerebral concussion as seen in the clinical setting. The pathological changes included cerebral vascular dilatation, congestion, edema of the cerebral tissues, neuronal degeneration, necrosis, and decrease or even disappearance of the Nissl bodies. The protein and mRNA of nNOS were increased 3 days after the injury, peaked on the 7th day, and decreased till the 14th days but still remained positive on the 30th day. The positive expression was detected in the plasma of neurons in the cerebral cortex, hippocampus, thalamus and cerebellum.CONCLUSION: Cerebral concussion is pathologically characterized by blood circulation disorder and neural cell degeneration and necrosis. The expression of nNOS gene participates in brain tissue damage in cerebral concussion, and may play an important role in regulating neural cell degeneration and necrosis.