CAJ | 학술논문

以2个马铃薯品种夏坡蒂和费乌瑞它茎段为外植体,研究了不同植物激素对马铃薯愈伤组织诱导及其不定芽分化的影响.结果表明,夏坡蒂和费乌瑞它的茎段最佳愈伤诱导组织培养基分别为MS+6-BA2.0mg/L+NAA0.5mg/L+GA_30.5mg/L和MS+ZT 2.0mg/L+NAA 0.1mg/L+GA_30.5mg/L,愈伤率均可达到100%;夏坡蒂和费乌瑞它的不定芽最佳诱导培养基分别为MS+ZT 2.0 mg/L+NAA0.1mg/L+GA_3 5mg/L和MS+6-BA 2.0mg/L+ZT 2.0mg/L+GA_3 5mg/L,诱导率分别为52.38%和53.6%.结果为进一步转基因工作中高效植株的再生奠定了一定的基础.
이2개마령서품충하파체화비오서타경단위외식체,연구료불동식물격소대마령서유상조직유도급기불정아분화적영향.결과표명,하파체화비오서타적경단최가유상유도조직배양기분별위MS+6-BA2.0mg/L+NAA0.5mg/L+GA_30.5mg/L화MS+ZT 2.0mg/L+NAA 0.1mg/L+GA_30.5mg/L,유상솔균가체도100%;하파체화비오서타적불정아최가유도배양기분별위MS+ZT 2.0 mg/L+NAA0.1mg/L+GA_3 5mg/L화MS+6-BA 2.0mg/L+ZT 2.0mg/L+GA_3 5mg/L,유도솔분별위52.38%화53.6%.결과위진일보전기인공작중고효식주적재생전정료일정적기출.
The stem explants of two potato varieties (Shepody and Favorite) were cultured on MS medium with different plant hormones to study the effects on callus induction and differentiation of adventitious buds. The results showed that the optimum medium for 100% callus induction rate of potato stem explants of Shepody and Favorite was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L +GA_3 0.5 mg/L and MS+ZT 2.0 mg/L+NAA 0.1 mg/L+GA_30.5 mg/L, the optimum medium for differentiation of adventitious buds of Shepody and Favorite was MS+ZT 2.0 mg/L+NAA 0.1 mg/L+GA_3 5 mg/L and MS+6-BA 2.0 mg/L+ ZT 2.0 mg/L+GA_3 5 mg/L and their differentiation rates of adventitious buds reached 52.38% (Shepody) and 53.6% (Favorite) respectively. The results lay a certain foundation for further efficient transgenic plant regeneration.