CAJ | 학술논문

作物抗性遗传研究可为抗性育种提供理论基础.在溧水中子黄豆×南农493-1杂交组合的244个F_(2∶3)家系中,随机取171个F_(2∶3)家系为材料,用150对SSR分子标记,通过JoinMap30软件构建了包括70对分子标记的25个连锁群;采用平均病级和综合病情指数两种指标,用Win QTL Cartographer V25软件的多区间作图法进行QTL定位.结果表明:大豆对大豆花叶病毒Sa株系的抗扩展平均病级和综合病情指数均有4个QTL,其中在C2-b连锁群的satt422-satt640标记间和D2-a连锁群有共同的QTL,两性状的4个和5个互作QTL可分别解释表型变异的1514%和526%.这些结果为抗性性状的遗传剖析和标记辅助育种提供理论依据.
작물항성유전연구가위항성육충제공이론기출.재률수중자황두×남농493-1잡교조합적244개F_(2∶3)가계중,수궤취171개F_(2∶3)가계위재료,용150대SSR분자표기,통과JoinMap30연건구건료포괄70대분자표기적25개련쇄군;채용평균병급화종합병정지수량충지표,용Win QTL Cartographer V25연건적다구간작도법진행QTL정위.결과표명:대두대대두화협병독Sa주계적항확전평균병급화종합병정지수균유4개QTL,기중재C2-b련쇄군적satt422-satt640표기간화D2-a련쇄군유공동적QTL,량성상적4개화5개호작QTL가분별해석표형변이적1514%화526%.저사결과위항성성상적유전부석화표기보조육충제공이론의거.
The genetic analysis for resistance traits is the foundation for resistance breeding in crop. One hundred and seventy-one F_(2:3) lines were randomly selected from 244 F_(2:3) lines derived from the cross between Lishuizhongzihuangdou and Nannong 493 - 1 and measured for resistance of soybean (Glycine max (L. ) Merr. ) to soybean mosaic virus (SMV) strain Sa, with two indicators including average disease degree and synthetic disease index. Among 150 SSR loci, a linkage map with twenty-five linkage groups was constructed with 70 SSR loci, covering 1 123. 99 cM. Therefore, an attempt was made to detect quantitative trait loci (QTL) for the two traits above by using multiple interval mapping in the software of Win QTL Cartographer V2. 5. The results showed that 4 QTLs were detected for each of the two traits above. Among these QTLs, 2 common QTL were identified, one is between satt422 and satt640 on the linkage group C2-b and the other is on the linkage group D2-a; and a total of 4 and 5 epistatic QTLs with 15. 14% and 52. 6% of the total phenotypic variance explained were detected for the two traits above, respectively. The results presented here will enhance the understanding of the genetic basis of the resistant traits and provide a solid theoretical foundation for soybean marker-assisted selection.